{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T16:22:32Z","timestamp":1785860552759,"version":"3.56.0"},"reference-count":40,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T00:00:00Z","timestamp":1697068800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003554","name":"Danish Biotechnology Center for Cellular Communication and the Lundbeck Foundation","doi-asserted-by":"publisher","award":["R347-2020-1920"],"award-info":[{"award-number":["R347-2020-1920"]}],"id":[{"id":"10.13039\/501100003554","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Pituitary adenylate cyclase-activating polypeptide (PACAP) is a naturally occurring neuropeptide found in both the central and peripheral nervous systems of vertebrates. Recent studies have revealed the presence of PACAP and its corresponding receptors, namely, the pituitary adenylate cyclase-activating polypeptide type I receptor (PAC1R), vasoactive intestinal peptide receptor 1 (VIPR1), and vasoactive intestinal peptide receptor 2 (VIPR2), in various structures implicated in migraine pathophysiology, including sensory trigeminal neurons. Human studies have demonstrated that when infused, PACAP can cause dilation of cranial vessels and result in delayed migraine-like attacks. In light of this, we present a novel ELISA assay that has been validated for quantifying PACAP in tissue extracts and human plasma. Using two well characterized antibodies specifically targeting PACAP, we successfully developed a sandwich ELISA assay, capable of detecting and accurately quantifying PACAP without any cross-reactivity to closely related peptides. The quantification range was between 5.2 pmol\/L and 400 pmol\/L. The recovery in plasma ranged from 98.2% to 100%. The increasing evidence pointing to the crucial role of PACAP in migraine pathophysiology necessitates the availability of tools capable of detecting changes in the circulatory levels of PACAP and its potential application as a reliable biomarker.<\/jats:p>","DOI":"10.3390\/ijms242015102","type":"journal-article","created":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T03:07:47Z","timestamp":1697080067000},"page":"15102","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Development of a New Enzyme-Linked Immunosorbent Assay (ELISA) for Measuring the Content of PACAP in Mammalian Tissue and Plasma"],"prefix":"10.3390","volume":"24","author":[{"given":"Elisabeth Anne Adanma","family":"Obara","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry, Faculty of Health Sciences, Bispebjerg and Frederiksberg Hospital, University of Copenhagen, Nielsine Nielsens Vej 4, 2400 Copenhagen, NV, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgitte","family":"Georg","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Faculty of Health Sciences, Bispebjerg and Frederiksberg Hospital, University of Copenhagen, Nielsine Nielsens Vej 4, 2400 Copenhagen, NV, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9416-0125","authenticated-orcid":false,"given":"Jens","family":"Hannibal","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Faculty of Health Sciences, Bispebjerg and Frederiksberg Hospital, University of Copenhagen, Nielsine Nielsens Vej 4, 2400 Copenhagen, NV, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"301","DOI":"10.2170\/jjphysiol.48.301","article-title":"Perspectives on pituitary adenylate cyclase activating polypeptide (PACAP) in the neuroendocrine, endocrine, and nervous systems","volume":"48","author":"Arimura","year":"1998","journal-title":"Jpn. J. Physiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/0006-291X(89)91757-9","article-title":"Isolation of a novel 38 residue-hypothalamic polypeptide which stimulates adenylate cyclase in pituitary cells","volume":"164","author":"Miyata","year":"1989","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1016\/0196-9781(95)02018-R","article-title":"Characterization of murine PACAP mRNA","volume":"16","author":"Okazaki","year":"1995","journal-title":"Peptides"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1016\/0006-291X(90)92140-U","article-title":"Isolation of a neuropeptide corresponding to the N-terminal 27 residues of the pituitary adenylate cyclase activating polypeptide with 38 residues (PACAP38)","volume":"170","author":"Miyata","year":"1990","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0167-0115(94)00099-J","article-title":"Gene expression of pituitary adenylate cyclase activating polypeptide (PACAP) in the rat hypothalamus","volume":"55","author":"Hannibal","year":"1995","journal-title":"Regul. Pept."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1006\/bbrc.1993.2423","article-title":"Molecular Cloning and Functional Expression of a cDNA Encoding a Human Pituitary Adenylate Cyclase Activating Polypeptide Receptor","volume":"196","author":"Ogi","year":"1993","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1016\/0896-6273(92)90101-I","article-title":"Functional expression and tissue distribution of a novel receptor for vasoactive intestinal polypeptide","volume":"8","author":"Ishihara","year":"1992","journal-title":"Neuron"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2679","DOI":"10.1073\/pnas.91.7.2679","article-title":"Cloning and functional characterization of a third pituitary adenylate cyclase-activating polypeptide receptor subtype expressed in insulin-secreting cells","volume":"91","author":"Inagaki","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1038\/365170a0","article-title":"Differential signal transduction by five splice variants of the PACAP receptor","volume":"365","author":"Spengler","year":"1993","journal-title":"Nature"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/s10194-018-0855-1","article-title":"Discovery of PACAP and its receptors in the brain","volume":"19","author":"Hirabayashi","year":"2018","journal-title":"J. Headache Pain"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1111\/j.1749-6632.1996.tb17501.x","article-title":"Pituitary adenylate cyclase-activating peptide in sensory and autonomic ganglia: Localization and regulation","volume":"805","author":"Sundler","year":"1996","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.brainres.2014.11.031","article-title":"Localization of CGRP, CGRP receptor, PACAP and glutamate in trigeminal ganglion. Relation to the blood-brain barrier","volume":"1600","author":"Eftekhari","year":"2015","journal-title":"Brain Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.neuroscience.2018.10.004","article-title":"Expression of Pituitary Adenylate Cyclase-activating Peptide, Calcitonin Gene-related Peptide and Headache Targets in the Trigeminal Ganglia of Rats and Humans","volume":"393","author":"Frederiksen","year":"2018","journal-title":"Neuroscience"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2105","DOI":"10.1016\/j.peptides.2004.08.002","article-title":"Peptidergic and non-peptidergic innervation and vasomotor responses of human lenticulostriate and posterior cerebral arteries","volume":"25","author":"Gulbenkian","year":"2004","journal-title":"Peptides"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/0006-8993(95)00869-R","article-title":"Concentrations of pituitary adenylate cyclase activating polypeptide (PACAP) in human brain nuclei","volume":"699","author":"Palkovits","year":"1995","journal-title":"Brain Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/0006-8993(93)90241-E","article-title":"Regional distribution of pituitary adenylate cyclase activating polypeptide (PACAP) in the rat central nervous system as determined by sandwich-enzyme immunoassay","volume":"602","author":"Masuo","year":"1993","journal-title":"Brain Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2787","DOI":"10.1210\/endo-129-5-2787","article-title":"Tissue Distribution of PACAP as Determined by RIA: Highly Abundant in the Rat Brain and Testes","volume":"129","author":"Arimura","year":"1991","journal-title":"Endocrinology"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1002\/cne.10418","article-title":"Pituitary adenylate cyclase-activating peptide in the rat central nervous system: An immunohistochemical and in situ hybridization study","volume":"453","author":"Hannibal","year":"2002","journal-title":"J. Comp. Neurol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1124\/pr.109.001370","article-title":"Pituitary adenylate cyclase-activating polypeptide and its receptors: 20 years after the discovery","volume":"61","author":"Vaudry","year":"2009","journal-title":"Pharmacol Rev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1007\/BF00318155","article-title":"Pituitary adenylate cyclase-activating peptide (PACAP), a new vasoactive intestinal peptide (VIP)-like peptide in the respiratory tract","volume":"265","author":"Uddman","year":"1991","journal-title":"Cell Tissue Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"145","DOI":"10.3389\/fendo.2012.00145","article-title":"Distribution and protective function of pituitary adenylate cyclase-activating polypeptide in the retina","volume":"3","author":"Nakamachi","year":"2012","journal-title":"Front. Endocrinol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1753","DOI":"10.1016\/j.peptides.2007.05.015","article-title":"Role of PACAP and VIP in astroglial functions","volume":"28","author":"Gandolfo","year":"2007","journal-title":"Peptides"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"973","DOI":"10.2174\/138161211795589346","article-title":"Role of PACAP in neural stem\/progenitor cell and astrocyte--from neural development to neural repair","volume":"17","author":"Nakamachi","year":"2011","journal-title":"Curr. Pharm. Des."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Hannibal, J., Georg, B., and Fahrenkrug, J. (2016). Altered Circadian Food Anticipatory Activity Rhythms in PACAP Receptor 1 (PAC1) Deficient Mice. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0146981"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.nbd.2008.06.014","article-title":"PACAP prevents toxicity induced by cisplatin in rat and primate neurons but not in proliferating ovary cells: Involvement of the mitochondrial apoptotic pathway","volume":"32","author":"Aubert","year":"2008","journal-title":"Neurobiol. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1111\/j.1749-6632.2003.tb03145.x","article-title":"PACAP in Immunity and Inflammation","volume":"992","author":"Delgado","year":"2003","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/0006-8993(96)00139-4","article-title":"Pituitary adenylate cyclase activating polypeptide-immunoreactivity in human spinal cord and dorsal root ganglia","volume":"721","author":"Dun","year":"1996","journal-title":"Brain Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"991403","DOI":"10.3389\/fnana.2022.991403","article-title":"Localization of the neuropeptides pituitary adenylate cyclase-activating polypeptide, vasoactive intestinal peptide, and their receptors in the basal brain blood vessels and trigeminal ganglion of the mouse CNS; an immunohistochemical study","volume":"16","author":"Lund","year":"2022","journal-title":"Front. Neuroanat."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1093\/brain\/awt369","article-title":"Investigation of the pathophysiological mechanisms of migraine attacks induced by pituitary adenylate cyclase-activating polypeptide-38","volume":"137","author":"Amin","year":"2014","journal-title":"Brain"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1177\/0333102411431333","article-title":"Headache and prolonged dilatation of the middle meningeal artery by PACAP38 in healthy volunteers","volume":"32","author":"Amin","year":"2012","journal-title":"Cephalalgia"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1093\/brain\/awn307","article-title":"PACAP38 induces migraine-like attacks in patients with migraine without aura","volume":"132","author":"Schytz","year":"2009","journal-title":"Brain"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1177\/0333102413483931","article-title":"Alterations in PACAP-38-like immunoreactivity in the plasma during ictal and interictal periods of migraine patients","volume":"33","author":"Tuka","year":"2013","journal-title":"Cephalalgia"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1448","DOI":"10.1111\/head.12949","article-title":"No Change in Interictal PACAP Levels in Peripheral Blood in Women with Chronic Migraine","volume":"56","author":"Riesco","year":"2016","journal-title":"Headache J. Head Face Pain"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"689939","DOI":"10.3389\/fnagi.2021.689939","article-title":"Correlation of Decreased Serum Pituitary Adenylate Cyclase-Activating Polypeptide and Vasoactive Intestinal Peptide Levels with Non-motor Symptoms in Patients with Parkinson\u2019s Disease","volume":"13","author":"Hu","year":"2021","journal-title":"Front. Aging Neurosci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"770980","DOI":"10.3389\/fneur.2021.770980","article-title":"Evaluation of Serum Levels of Transient Receptor Potential Cation Channel Subfamily V Member 1, Vasoactive Intestinal Polypeptide, and Pituitary Adenylate Cyclase-Activating Polypeptide in Chronic and Episodic Migraine: The Possible Role in Migraine Transformation","volume":"12","author":"Togha","year":"2021","journal-title":"Front. Neurol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"22418","DOI":"10.1074\/jbc.M212355200","article-title":"The role of dipeptidyl peptidase IV in the cleavage of glucagon family peptides: In vivo metabolism of pituitary adenylate cyclase activating polypeptide-(1-38)","volume":"278","author":"Zhu","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.regpep.2006.12.010","article-title":"The effect of intravenous PACAP38 on cerebral hemodynamics in healthy volunteers","volume":"140","author":"Birk","year":"2007","journal-title":"Regul. Pept."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0167-0115(99)00089-0","article-title":"Dipeptidyl-peptidase IV (CD26)\u2014Role in the inactivation of regulatory peptides","volume":"85","author":"Mentlein","year":"1999","journal-title":"Regul. Pept."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1042\/bj3410271","article-title":"Identification of pituitary adenylate cyclase-activating polypeptide1-38-binding factor in human plasma, as ceruloplasmin","volume":"341","author":"Tams","year":"1999","journal-title":"Biochem. J."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Hannibal, J., Georg, B., and Fahrenkrug, J. (2017). PAC1- and VPAC2 receptors in light regulated behavior and physiology: Studies in single and double mutant mice. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0188166"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/20\/15102\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:05:18Z","timestamp":1760130318000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/20\/15102"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,12]]},"references-count":40,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2023,10]]}},"alternative-id":["ijms242015102"],"URL":"https:\/\/doi.org\/10.3390\/ijms242015102","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,12]]}}}