{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:33:26Z","timestamp":1760150006995,"version":"build-2065373602"},"reference-count":30,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T00:00:00Z","timestamp":1697068800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["03IPT614A","421152132","EXC 2008\u2014390540038","956314 [ALLODD]"],"award-info":[{"award-number":["03IPT614A","421152132","EXC 2008\u2014390540038","956314 [ALLODD]"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Deutsche Forschungsgemeinschaft (DFG) (German Research Foundation)","award":["03IPT614A","421152132","EXC 2008\u2014390540038","956314 [ALLODD]"],"award-info":[{"award-number":["03IPT614A","421152132","EXC 2008\u2014390540038","956314 [ALLODD]"]}]},{"name":"Germany\u2019s Excellence Strategy","award":["03IPT614A","421152132","EXC 2008\u2014390540038","956314 [ALLODD]"],"award-info":[{"award-number":["03IPT614A","421152132","EXC 2008\u2014390540038","956314 [ALLODD]"]}]},{"name":"European Union\u2019s Horizon 2020 research and innovation programme","award":["03IPT614A","421152132","EXC 2008\u2014390540038","956314 [ALLODD]"],"award-info":[{"award-number":["03IPT614A","421152132","EXC 2008\u2014390540038","956314 [ALLODD]"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>G protein-coupled receptor 83 (GPR83) is a class A G protein-coupled receptor with predominant expression in the cerebellum and proposed function in the regulation of food intake and in anxiety-like behavior. The neuropeptide PEN has been suggested as a specific GPR83 ligand. However, conflicting reports exist about whether PEN is indeed able to bind and activate GPR83. This study was initiated to evaluate PEN as a potential ligand of GPR83. Employing several second messenger and other GPCR activation assays as well as a radioligand binding assay, and using multiple GPR83 plasmids and PEN peptides from different sources, no experimental evidence was found to support a role of PEN as a GPR83 ligand.<\/jats:p>","DOI":"10.3390\/ijms242015117","type":"journal-article","created":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T07:28:44Z","timestamp":1697095724000},"page":"15117","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Is the Neuropeptide PEN a Ligand of GPR83?"],"prefix":"10.3390","volume":"24","author":[{"given":"Yvonne","family":"Giesecke","sequence":"first","affiliation":[{"name":"Tumor Targeting Group, Department of Hepatology and Gastroenterology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, 13353 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vahid","family":"Asimi","sequence":"additional","affiliation":[{"name":"Tumor Targeting Group, Department of Hepatology and Gastroenterology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, 13353 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"Stulberg","sequence":"additional","affiliation":[{"name":"Institute of Chemistry and Biochemistry, Freie Universit\u00e4t Berlin, 14195 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gunnar","family":"Kleinau","sequence":"additional","affiliation":[{"name":"Group Structural Biology of Cellular Signaling, Institute of Medical Physics and Biophysics, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, 10117 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5028-2075","authenticated-orcid":false,"given":"Patrick","family":"Scheerer","sequence":"additional","affiliation":[{"name":"Group Structural Biology of Cellular Signaling, Institute of Medical Physics and Biophysics, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, 10117 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beate","family":"Koksch","sequence":"additional","affiliation":[{"name":"Institute of Chemistry and Biochemistry, Freie Universit\u00e4t Berlin, 14195 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9872-3087","authenticated-orcid":false,"given":"Carsten","family":"Gr\u00f6tzinger","sequence":"additional","affiliation":[{"name":"Tumor Targeting Group, Department of Hepatology and Gastroenterology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, 13353 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1486","DOI":"10.1016\/j.metabol.2012.03.015","article-title":"Downregulation of GPR83 in the hypothalamic preoptic area reduces core body temperature and elevates circulating levels of adiponectin","volume":"61","author":"Dubins","year":"2012","journal-title":"Metabolism"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1968","DOI":"10.1038\/ncomms2968","article-title":"The orphan receptor Gpr83 regulates systemic energy metabolism via ghrelin-dependent and ghrelin-independent mechanisms","volume":"4","author":"Muller","year":"2013","journal-title":"Nat. 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