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Pain is one of the pathophysiologically incompletely understood symptoms in FD patients. Previous data suggest an involvement of hypoxia and mitochondriopathy in FD pain development at dorsal root ganglion (DRG) level. Using immunofluorescence and quantitative real-time polymerase chain reaction (qRT PCR), we investigated patient-derived endothelial cells (EC) and DRG tissue of the GLA knockout (KO) mouse model of FD. We address the question of whether hypoxia and mitochondriopathy contribute to FD pain pathophysiology. In EC of FD patients (P1 with pain and, P2 without pain), we found dysregulated protein expression of hypoxia-inducible factors (HIF) 1a and HIF2 compared to the control EC (p &lt; 0.01). The protein expression of the HIF downstream target vascular endothelial growth factor A (VEGFA, p &lt; 0.01) was reduced and tube formation was hampered in the P1 EC compared to the healthy EC (p &lt; 0.05). Tube formation ability was rescued by applying transforming growth factor beta (TGF\u03b2) inhibitor SB-431542. Additionally, we found dysregulated mitochondrial fusion\/fission characteristics in the P1 and P2 EC (p &lt; 0.01) and depolarized mitochondrial membrane potential in P2 compared to control EC (p &lt; 0.05). Complementary to human data, we found upregulated hypoxia-associated genes in the DRG of old GLA KO mice compared to WT DRG (p &lt; 0.01). At protein level, nuclear HIF1a was higher in the DRG neurons of old GLA KO mice compared to WT mice (p &lt; 0.01). Further, the HIF1a downstream target CA9 was upregulated in the DRG of old GLA KO mice compared to WT DRG (p &lt; 0.01). Similar to human EC, we found a reduction in the vascular characteristics in GLA KO DRG compared to WT (p &lt; 0.05). We demonstrate increased hypoxia, impaired vascular properties, and mitochondrial dysfunction in human FD EC and complementarily at the GLA KO mouse DRG level. Our data support the hypothesis that hypoxia and mitochondriopathy in FD EC and GLA KO DRG may contribute to FD pain development.<\/jats:p>","DOI":"10.3390\/ijms242015422","type":"journal-article","created":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T12:59:01Z","timestamp":1697893141000},"page":"15422","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Endothelial Cell Dysfunction and Hypoxia as Potential Mediators of Pain in Fabry Disease: A Human-Murine Translational Approach"],"prefix":"10.3390","volume":"24","author":[{"given":"Katharina","family":"Klug","sequence":"first","affiliation":[{"name":"Department of Neurology, University Hospital of W\u00fcrzburg, Josef-Schneider-Str. 11, 97080 W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlene","family":"Spitzel","sequence":"additional","affiliation":[{"name":"Department of Neurology, University Hospital of W\u00fcrzburg, Josef-Schneider-Str. 11, 97080 W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Hans","sequence":"additional","affiliation":[{"name":"Department of Neurology, University Hospital of W\u00fcrzburg, Josef-Schneider-Str. 11, 97080 W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9486-4163","authenticated-orcid":false,"given":"Alexandra","family":"Klein","sequence":"additional","affiliation":[{"name":"Department of Neurology, University Hospital of W\u00fcrzburg, Josef-Schneider-Str. 11, 97080 W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole Michelle","family":"Schottmann","sequence":"additional","affiliation":[{"name":"Department of Neurology, University Hospital of W\u00fcrzburg, Josef-Schneider-Str. 11, 97080 W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Erbacher","sequence":"additional","affiliation":[{"name":"Department of Neurology, University Hospital of W\u00fcrzburg, Josef-Schneider-Str. 11, 97080 W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6973-6428","authenticated-orcid":false,"given":"Nurcan","family":"\u00dc\u00e7eyler","sequence":"additional","affiliation":[{"name":"Department of Neurology, University Hospital of W\u00fcrzburg, Josef-Schneider-Str. 11, 97080 W\u00fcrzburg, Germany"},{"name":"W\u00fcrzburg Fabry Center for Interdisciplinary Therapy (FAZIT), University Hospital of W\u00fcrzburg, 97080 W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1111\/cge.14102","article-title":"Challenging the traditional approach for interpreting genetic variants: Lessons from Fabry disease","volume":"101","author":"Germain","year":"2022","journal-title":"Clin. 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