{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:07:16Z","timestamp":1791389236802,"version":"4.3.3"},"reference-count":117,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T00:00:00Z","timestamp":1698019200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Deanship of Scientific Research, Vice Presidency for Graduate Studies and Scientific Research, King Faisal University, Saudi Arabia","award":["GRANT4583"],"award-info":[{"award-number":["GRANT4583"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Diabetes mellitus is a metabolic disorder associated with various complications encompassing male reproductive dysfunction. The present study aimed to investigate the therapeutic potential of biologically active Lepidium sativum seed oil (LSO) against the testicular dysfunction associated with streptozotocin (STZ)-induced diabetes. Male adults (n = 24) were divided into four groups: control, LSO-administered, diabetic (D), and LSO-treated diabetic (D+LSO) groups. LSO was extracted from L. sativum seeds, and its chemical composition was determined using GC-MS. Serum testosterone levels, testicular enzymatic antioxidants (catalase (CAT) and superoxide dismutase (SOD)), an oxidative stress (OS) biomarker, malondialdehyde (MDA), pro-inflammatory markers (NF-kB, IL-1, IL-6, and TNF-\u03b1), and the expression level of NF-kB were assessed. In addition, histopathological changes were evaluated in testicular tissues. The results obtained showed that the chemical composition of LSO indicated its enrichment mainly with \u03b3-tocopherol (62.1%), followed by 2-methylhexacosane (8.12%), butylated hydroxytoluene (8.04%), 10-Methylnonadecane (4.81%), and \u03b4-tocopherol (3.91%). Moreover, LSO administration in the D+LSO mice significantly increased testosterone levels and ameliorated the observed testicular oxidative damage, inflammatory response, and reduced NF-kB expression compared to the diabetic mice. Biochemical and molecular analyses confirmed the histological results. In conclusion, LSO may prevent the progression of diabetes-induced impairment in the testes through inhibition of the OS- and NF-kB-mediated inflammatory response.<\/jats:p>","DOI":"10.3390\/ijms242015478","type":"journal-article","created":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T10:32:24Z","timestamp":1698057144000},"page":"15478","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Garden Cress Seed Oil Abrogates Testicular Oxidative Injury and NF-kB-Mediated Inflammation in Diabetic Mice"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1300-3150","authenticated-orcid":false,"given":"Rasha","family":"Abu-Khudir","sequence":"first","affiliation":[{"name":"Department of Chemistry, College of Science, King Faisal University, P.O. Box 380, Al-Ahsa 31982, Saudi Arabia"},{"name":"Department of Chemistry, Biochemistry Division, Faculty of Science, Tanta University, Tanta 31527, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gehan M.","family":"Badr","sequence":"additional","affiliation":[{"name":"Department of Zoology, Faculty of Science, Ain Shams University, Cairo 11566, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5665-7592","authenticated-orcid":false,"given":"Heba Ibrahim","family":"Abd El-Moaty","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, College of Science, King Faisal University, P.O. Box 380, Al-Ahsa 31982, Saudi Arabia"},{"name":"Medicinal and Aromatic Plants Department, Desert Research Center El-Mataria, Cairo 11753, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3465-4470","authenticated-orcid":false,"given":"Rabab S.","family":"Hamad","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, College of Science, King Faisal University, P.O. Box 380, Al-Ahsa 31982, Saudi Arabia"},{"name":"Central Laboratory, Theodor Bilharz Research Institute, Giza 12411, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Najla K.","family":"Al Abdulsalam","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, College of Science, King Faisal University, P.O. Box 380, Al-Ahsa 31982, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aml Sayed Ali","family":"Abdelrahem","sequence":"additional","affiliation":[{"name":"Department of Nursing, College of Applied Medical Science, King Faisal University, P.O. Box 380, Al-Ahsa 31982, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5422-6074","authenticated-orcid":false,"given":"Saleha","family":"Alqarni","sequence":"additional","affiliation":[{"name":"Department of Clinical Nutrition, College of Applied Medical Science King Faisal University, P.O. Box 380, Al-Ahsa 31982, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8048-7378","authenticated-orcid":false,"given":"Mayyadah Abdullah","family":"Alkuwayti","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, College of Science, King Faisal University, P.O. Box 380, Al-Ahsa 31982, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherine Abdel","family":"Salam","sequence":"additional","affiliation":[{"name":"Department of Zoology, Faculty of Science, Alexandria University, Alexandria 21511, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3901-3696","authenticated-orcid":false,"given":"Hanaa F.","family":"Abd El-Kareem","sequence":"additional","affiliation":[{"name":"Department of Zoology, Faculty of Science, Ain Shams University, Cairo 11566, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Henninger, J., Hammarstedt, A., Rawshani, A., and Eliasson, B. (2015). Metabolic Predictors of Impaired Glucose Tolerance and Type 2 Diabetes in a Predisposed Population\u2014A Prospective Cohort Study. BMC Endocr. 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