{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T04:14:06Z","timestamp":1781151246380,"version":"3.54.1"},"reference-count":39,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T00:00:00Z","timestamp":1698105600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["U1812403"],"award-info":[{"award-number":["U1812403"]}]},{"name":"National Natural Science Foundation of China","award":["81760573"],"award-info":[{"award-number":["81760573"]}]},{"name":"National Natural Science Foundation of China","award":["QKHZYD (2022)4015"],"award-info":[{"award-number":["QKHZYD (2022)4015"]}]},{"name":"National Natural Science Foundation of China","award":["QKHJC-ZK (2023)YB316"],"award-info":[{"award-number":["QKHJC-ZK (2023)YB316"]}]},{"name":"National Natural Science Foundation of China","award":["KY (2020)249"],"award-info":[{"award-number":["KY (2020)249"]}]},{"name":"Department of Science and Technology of Guizhou Province","award":["U1812403"],"award-info":[{"award-number":["U1812403"]}]},{"name":"Department of Science and Technology of Guizhou Province","award":["81760573"],"award-info":[{"award-number":["81760573"]}]},{"name":"Department of Science and Technology of Guizhou Province","award":["QKHZYD (2022)4015"],"award-info":[{"award-number":["QKHZYD (2022)4015"]}]},{"name":"Department of Science and Technology of Guizhou Province","award":["QKHJC-ZK (2023)YB316"],"award-info":[{"award-number":["QKHJC-ZK (2023)YB316"]}]},{"name":"Department of Science and Technology of Guizhou Province","award":["KY (2020)249"],"award-info":[{"award-number":["KY (2020)249"]}]},{"name":"Joint Open Fund Program for Key Laboratories of the Ministry of Education of China","award":["U1812403"],"award-info":[{"award-number":["U1812403"]}]},{"name":"Joint Open Fund Program for Key Laboratories of the Ministry of Education of China","award":["81760573"],"award-info":[{"award-number":["81760573"]}]},{"name":"Joint Open Fund Program for Key Laboratories of the Ministry of Education of China","award":["QKHZYD (2022)4015"],"award-info":[{"award-number":["QKHZYD (2022)4015"]}]},{"name":"Joint Open Fund Program for Key Laboratories of the Ministry of Education of China","award":["QKHJC-ZK (2023)YB316"],"award-info":[{"award-number":["QKHJC-ZK (2023)YB316"]}]},{"name":"Joint Open Fund Program for Key Laboratories of the Ministry of Education of China","award":["KY (2020)249"],"award-info":[{"award-number":["KY (2020)249"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Arsenic is a carcinogenic metalloid toxicant widely found in the natural environment. Acute or prolonged exposure to arsenic causes a series of damages to the organs, mainly the liver, such as hepatomegaly, liver fibrosis, cirrhosis, and even hepatocellular carcinoma. Therefore, it is imperative to seek drugs to prevent arsenic-induced liver injury. Quinazolines are a class of nitrogen heterocyclic compounds with biological and pharmacological effects in vivo and in vitro. This study was designed to investigate the ameliorating effects of quinazoline derivatives on arsenic-induced liver injury and its molecular mechanism. We investigated the mechanism of the quinazoline derivative KZL-047 in preventing and ameliorating arsenic-induced liver injury in vitro by cell cycle and apoptosis. We performed real-time fluorescence quantitative polymerase chain reaction (qPCR) and Western blotting combined with molecular docking. In vivo, the experiments were performed to investigate the mechanism of KZL-047 in preventing and ameliorating arsenic-induced liver injury using arsenic-infected mice. Physiological and biochemical indices of liver function in mouse serum were measured, histopathological changes in liver tissue were observed, and immunohistochemical staining was used to detect changes in the expression of RecQ-family helicases in mouse liver tissue. The results of in vitro experiments showed that sodium arsenite (SA) inhibited the proliferation of L-02 cells, induced apoptosis, blocked the cell cycle at the G1 phase, and decreased the expression of RecQ family helicase; after KZL-047 treatment in arsenic-induced L-02 cells, the expression of RecQ family helicase was upregulated, and the apoptosis rate was slowed, leading to the restoration of the cell viability level. KZL-047 inhibited arsenic-induced oxidative stress, alleviated oxidative damage and lipid peroxidation in vivo, and ameliorated arsenic toxicity-induced liver injury. KZL-047 restored the expression of RecQ family helicase proteins, which is consistent with the results of in vitro studies. In summary, KZL-047 can be considered a potential candidate for the treatment of arsenic-induced liver injury.<\/jats:p>","DOI":"10.3390\/ijms242115521","type":"journal-article","created":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T06:28:25Z","timestamp":1698128905000},"page":"15521","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A Novel Quinazoline Derivative Prevents and Treats Arsenic-Induced Liver Injury by Regulating the Expression of RecQ Family Helicase"],"prefix":"10.3390","volume":"24","author":[{"given":"Heping","family":"Yang","sequence":"first","affiliation":[{"name":"The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, China"},{"name":"State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Mo","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, China"},{"name":"State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Langlang","family":"Yang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Yu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China"},{"name":"Natural Products Research Center of Guizhou Province, Guizhou Medical University, Guiyang 550014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China"},{"name":"Natural Products Research Center of Guizhou Province, Guizhou Medical University, Guiyang 550014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sha","family":"Cheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China"},{"name":"Natural Products Research Center of Guizhou Province, Guizhou Medical University, Guiyang 550014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baofei","family":"Sun","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, China"},{"name":"State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bixue","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China"},{"name":"Natural Products Research Center of Guizhou Province, Guizhou Medical University, Guiyang 550014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aihua","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng","family":"Luo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China"},{"name":"Natural Products Research Center of Guizhou Province, Guizhou Medical University, Guiyang 550014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2073","DOI":"10.1002\/tox.23324","article-title":"Imbalanced inflammatory response in subchronic arsenic-induced liver injury and the protective effects of Ginkgo biloba extract in rats: Potential role of cytokines mediated cell\u2013cell interactions","volume":"36","author":"Dong","year":"2021","journal-title":"Environ. 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