{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T19:48:53Z","timestamp":1792007333839,"version":"4.4.0"},"reference-count":59,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T00:00:00Z","timestamp":1698192000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"publisher","award":["310030_179169\/1"],"award-info":[{"award-number":["310030_179169\/1"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Satellite glial cells (SGCs), enveloping primary sensory neurons\u2019 somas in the dorsal root ganglion (DRG), contribute to neuropathic pain upon nerve injury. Glial fibrillary acidic protein (GFAP) serves as an SGC activation marker, though its DRG satellite cell specificity is debated. We employed the hGFAP-CFP transgenic mouse line, designed for astrocyte studies, to explore its expression within the peripheral nervous system (PNS) after spared nerve injury (SNI). We used diverse immunostaining techniques, Western blot analysis, and electrophysiology to evaluate GFAP+ cell changes. Post-SNI, GFAP+ cell numbers increased without proliferation, and were found near injured ATF3+ neurons. GFAP+ FABP7+ SGCs increased, yet 75.5% of DRG GFAP+ cells lacked FABP7 expression. This suggests a significant subset of GFAP+ cells are non-myelinating Schwann cells (nmSC), indicated by their presence in the dorsal root but not in the ventral root which lacks unmyelinated fibres. Additionally, patch clamp recordings from GFAP+ FABP7\u2212cells lacked SGC-specific Kir4.1 currents, instead displaying outward Kv currents expressing Kv1.1 and Kv1.6 channels specific to nmSCs. In conclusion, this study demonstrates increased GFAP expression in two DRG glial cell subpopulations post-SNI: GFAP+ FABP7+ SGCs and GFAP+ FABP7\u2212 nmSCs, shedding light on GFAP\u2019s specificity as an SGC marker after SNI.<\/jats:p>","DOI":"10.3390\/ijms242115559","type":"journal-article","created":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T04:14:47Z","timestamp":1698207287000},"page":"15559","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Characterisation of GFAP-Expressing Glial Cells in the Dorsal Root Ganglion after Spared Nerve Injury"],"prefix":"10.3390","volume":"24","author":[{"given":"Elena A.","family":"Konnova","sequence":"first","affiliation":[{"name":"Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV), 1005 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandru-Florian","family":"Deftu","sequence":"additional","affiliation":[{"name":"Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV), 1005 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0029-0947","authenticated-orcid":false,"given":"Paul","family":"Chu Sin Chung","sequence":"additional","affiliation":[{"name":"Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV), 1005 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Pertin","sequence":"additional","affiliation":[{"name":"Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV), 1005 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7764-2453","authenticated-orcid":false,"given":"Guyl\u00e8ne","family":"Kirschmann","sequence":"additional","affiliation":[{"name":"Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV), 1005 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4820-5289","authenticated-orcid":false,"given":"Isabelle","family":"Decosterd","sequence":"additional","affiliation":[{"name":"Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV), 1005 Lausanne, Switzerland"},{"name":"Department of Fundamental Neurosciences, Faculty of Biology and Medicine, University of Lausanne, 1005 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4653-0512","authenticated-orcid":false,"given":"Marc R.","family":"Suter","sequence":"additional","affiliation":[{"name":"Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV), 1005 Lausanne, Switzerland"},{"name":"Department of Fundamental Neurosciences, Faculty of Biology and Medicine, University of Lausanne, 1005 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1017\/S1740925X08000100","article-title":"Do glial cells control pain?","volume":"3","author":"Suter","year":"2007","journal-title":"Neuron Glia Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S10","DOI":"10.1016\/j.pain.2013.06.022","article-title":"Glia and pain: Is chronic pain a gliopathy?","volume":"154","author":"Ji","year":"2013","journal-title":"Pain"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1126\/science.aaf8924","article-title":"Pain regulation by non-neuronal cells and inflammation","volume":"354","author":"Ji","year":"2016","journal-title":"Science"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2047","DOI":"10.1002\/cne.24667","article-title":"Morphological characteristics of p75 neurotrophin receptor-positive cells define a new type of glial cell in the rat dorsal root ganglia","volume":"527","author":"Koike","year":"2019","journal-title":"J. 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