{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T13:49:12Z","timestamp":1767016152884,"version":"build-2065373602"},"reference-count":62,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T00:00:00Z","timestamp":1698278400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100017638","name":"Ministry of Health of the Russian Federation","doi-asserted-by":"publisher","award":["\u2116 121040600435-0"],"award-info":[{"award-number":["\u2116 121040600435-0"]}],"id":[{"id":"10.13039\/501100017638","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Antihypertensive therapy is an essential part of management of patients with preeclampsia (PE). Methyldopa (Dopegyt\u00ae) and nifedipine (Cordaflex\u00ae) are basic medications of therapy since they stabilize blood pressure without affecting the fetus. Their effect on the endothelium of placental vessels has not yet been studied. In this study, we analyzed the effect of antihypertensive therapy on the expression of fucosylated glycans in fetal capillaries of placental terminal villi in patients with early-onset PE (EOPE) and late-onset PE (LOPE), and determined correlation between their expression and mother\u2019s hemodynamic parameters, fetoplacental system, factors reflecting inflammatory response, and destructive processes in the endothelial glycocalyx (eGC). A total of 76 women were enrolled in the study: the comparison group consisted of 15 women with healthy pregnancy, and the main group comprised 61 women with early-onset and late-onset PE, who received one-component or two-component antihypertensive therapy. Hemodynamic status was assessed by daily blood pressure monitoring, dopplerometry of maternal placental and fetoplacental blood flows, and the levels of IL-18, IL-6, TNF\u03b1, galectin-3, endocan-1, syndecan-1, and hyaluronan in the blood of the mother. Expression of fucosylated glycans was assessed by staining placental sections with AAL, UEA-I, LTL lectins, and anti-LeY MAbs. It was found that (i) expression patterns of fucosylated glycans in eGC capillaries of placental terminal villi in EOPE and LOPE are characterized by predominant expression of structures with a type 2 core and have a similar pattern of quantitative changes, which seems to be due to the impact of one-component and two-component antihypertensive therapy on their expression; (ii) correlation patterns indicate interrelated changes in the molecular composition of eGC fucoglycans and indicators reflecting changes in maternal hemodynamics, fetoplacental hemodynamics, and humoral factors associated with eGC damage. The presented study is the first to demonstrate the features of placental eGC in women with PE treated with antihypertensive therapy. This study also considers placental fucoglycans as a functional part of the eGC, which affects hemodynamics in the mother\u2013placenta\u2013fetus system.<\/jats:p>","DOI":"10.3390\/ijms242115611","type":"journal-article","created":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T07:22:15Z","timestamp":1698304935000},"page":"15611","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Features and Comparative Characteristics of Fucosylated Glycans Expression in Endothelial Glycocalyx of Placental Terminal Villi in Patients with Preeclampsia Treated with Different Antihypertensive Regimens"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1578-8403","authenticated-orcid":false,"given":"Marina M.","family":"Ziganshina","sequence":"first","affiliation":[{"name":"Laboratory of Clinical Immunology, National Medical Research Center for Obstetrics, Gynecology, and Perinatology Named after Academician V.I. Kulakov of the Ministry of Health of the Russian Federation, Oparina Str. 4, 117997 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Galina V.","family":"Kulikova","sequence":"additional","affiliation":[{"name":"Department of Perinatal Pathology, National Medical Research Center for Obstetrics, Gynecology, and Perinatology Named after Academician V.I. Kulakov of the Ministry of Health of the Russian Federation, Oparina Str. 4, 117997 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamilla T.","family":"Muminova","sequence":"additional","affiliation":[{"name":"High Risk Pregnancy Department, National Medical Research Center for Obstetrics, Gynecology, and Perinatology Named after Academician V.I. Kulakov of the Ministry of Health of the Russian Federation, Oparina Str. 4, 117997 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2111-1530","authenticated-orcid":false,"given":"Alexander I.","family":"Shchegolev","sequence":"additional","affiliation":[{"name":"Department of Perinatal Pathology, National Medical Research Center for Obstetrics, Gynecology, and Perinatology Named after Academician V.I. Kulakov of the Ministry of Health of the Russian Federation, Oparina Str. 4, 117997 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ekaterina L.","family":"Yarotskaya","sequence":"additional","affiliation":[{"name":"Department of International Cooperation, National Medical Research Center for Obstetrics, Gynecology, and Perinatology Named after Academician V.I. Kulakov of the Ministry of Health of the Russian Federation, Oparina Str. 4, 117997 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8159-3714","authenticated-orcid":false,"given":"Zulfiya S.","family":"Khodzhaeva","sequence":"additional","affiliation":[{"name":"High Risk Pregnancy Department, National Medical Research Center for Obstetrics, Gynecology, and Perinatology Named after Academician V.I. Kulakov of the Ministry of Health of the Russian Federation, Oparina Str. 4, 117997 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gennady T.","family":"Sukhikh","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Immunology, National Medical Research Center for Obstetrics, Gynecology, and Perinatology Named after Academician V.I. Kulakov of the Ministry of Health of the Russian Federation, Oparina Str. 4, 117997 Moscow, Russia"},{"name":"Department of Obstetrics, Gynecology, Perinatology and Reproductology, Faculty for Postgraduate and Advanced Training of Physicians, I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), 119991 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1055\/s-0041-1729953","article-title":"Preeclampsia: Universal Screening or Universal Prevention for Low and Middle-Income Settings?","volume":"43","author":"Rolink","year":"2021","journal-title":"Rev. Bras. Ginecol. 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