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host immune evasion. Streptococcus sanguinis, a pioneer colonizer of teeth commonly involved in cardiovascular infections, expresses about thirty-three proteins anchored to the cell wall by sortase A. Here, we characterized the production of amyloid in S. sanguinis strains differing in biofilm and immune evasion phenotypes and investigated the role of sortase A in amyloidogenesis. Amyloid was identified in biofilms formed by nine strains, using Congo red (CR) staining and cross-polarized light microscopy. Additionally, EGCG, an amyloid inhibitor, impaired biofilm maturation in a strain-specific fashion. The amounts of amyloid-like components quantified in culture fluids of nine strains using thioflavin T and fluorimetry negatively correlated with bacterial binding to complement-activating proteins (SAP, C1q), C3b deposition and rates of opsonophagocytosis in PMNs, implying amyloid production in immune evasion. The deletion of the sortase A gene (srtA) in strain SK36 compromised amyloid production and sucrose-independent biofilm maturation. The srtA mutant further showed increased susceptibility to C3b deposition and altered interactions with PMNs as well as reduced persistence in human blood. These findings highlight the contribution of amyloids to biofilm formation and host immune evasion in S. sanguinis strains, further indicating the participation of sortase A substrates in amyloidogenesis.<\/jats:p>","DOI":"10.3390\/ijms242115686","type":"journal-article","created":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T09:27:28Z","timestamp":1698658048000},"page":"15686","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Amyloid Fibrils Produced by Streptococcus sanguinis Contribute to Biofilm Formation and Immune Evasion"],"prefix":"10.3390","volume":"24","author":[{"given":"Eduardo M.","family":"Franco","sequence":"first","affiliation":[{"name":"Department of Oral Diagnosis, Piracicaba Dental School, State University of Campinas, Piracicaba 13414-903, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2312-5685","authenticated-orcid":false,"given":"L\u00edvia A.","family":"Alves","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis, Piracicaba Dental School, State University of Campinas, Piracicaba 13414-903, SP, Brazil"},{"name":"School of Dentistry, Cruzeiro do Sul University (UNICSUL), S\u00e3o Paulo 01506-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2688-8488","authenticated-orcid":false,"given":"Hassan","family":"Naveed","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis, Piracicaba Dental School, State University of Campinas, Piracicaba 13414-903, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7942-6367","authenticated-orcid":false,"given":"Victor A. 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Oral Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"915","DOI":"10.2217\/fmb-2018-0043","article-title":"Streptococcus sanguinis Biofilm Formation & Interaction with Oral Pathogens","volume":"13","author":"Zhu","year":"2018","journal-title":"Future Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"505","DOI":"10.7326\/0003-4819-94-4-505","article-title":"Infective Endocarditis: An Analysis Based on Strict Case Definitions","volume":"94","author":"Levy","year":"1981","journal-title":"Ann. Intern. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1099\/00222615-39-3-179","article-title":"Identity of viridans streptococci isolated from cases of infective endocarditis","volume":"39","author":"Douglas","year":"1993","journal-title":"J. Med. Microbiol."},{"key":"ref_5","first-page":"e24189","article-title":"Streptococcus sanguinis Endocarditis of Bicuspid Aortic Valve Presenting as Septic Arthritis of Lumbar Facet Joint","volume":"14","author":"Kovuri","year":"2022","journal-title":"Cureus"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3313","DOI":"10.1128\/JCM.00377-06","article-title":"Detection of Cariogenic Streptococcus mutans in Extirpated Heart Valve and Atheromatous Plaque Specimens","volume":"44","author":"Nakano","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3166","DOI":"10.1128\/JB.01808-06","article-title":"Genome of the Opportunistic Pathogen Streptococcus sanguinis","volume":"189","author":"Xu","year":"2007","journal-title":"J. Bacteriol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4966","DOI":"10.1128\/IAI.00760-09","article-title":"Comprehensive Evaluation of Streptococcus sanguinis Cell Wall-Anchored Proteins in Early Infective Endocarditis","volume":"77","author":"Turner","year":"2009","journal-title":"Infect. Immun."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1099\/jmm.0.000955","article-title":"Oral streptococci show diversity in resistance to complement immunity","volume":"68","author":"Alves","year":"2019","journal-title":"J. Med. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1111\/j.1399-302X.2006.00272.x","article-title":"Human T-cell responses to oral streptococci in human PBMC-NOD\/SCID mice","volume":"21","author":"Salam","year":"2006","journal-title":"Oral Microbiol. Immunol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Lee, Y.H., Zimmerman, J.N., Custodio, W., Xiao, Y., Basiri, T., Hatibovic-Kofman, S., and Siqueira, W.L. (2013). Proteomic Evaluation of Acquired Enamel Pellicle during In Vivo Formation. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0067919"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e01262-20","DOI":"10.1128\/AEM.01262-20","article-title":"Competition and Caries on Enamel of a Dual-Species Biofilm Model with Streptococcus mutans and Streptococcus sanguinis","volume":"86","author":"Lozano","year":"2020","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"7193","DOI":"10.1128\/JB.187.21.7193-7203.2005","article-title":"Competition and Coexistence between Streptococcus mutans and Streptococcus sanguinis in the Dental Biofilm","volume":"187","author":"Kreth","year":"2005","journal-title":"J. Bacteriol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"6281","DOI":"10.1128\/JB.00906-09","article-title":"Characterization of Hydrogen Peroxide-Induced DNA Release by Streptococcus sanguinis and Streptococcus gordonii","volume":"191","author":"Kreth","year":"2009","journal-title":"J. Bacteriol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4941","DOI":"10.1128\/IAI.01850-14","article-title":"Two-Component System VicRK Regulates Functions Associated with Establishment of Streptococcus sanguinis in Biofilms","volume":"82","author":"Moraes","year":"2014","journal-title":"Infect. Immun."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1111\/apm.12238","article-title":"The influence of a glucosyltransferase, encoded by gtfP, on biofilm formation by Streptococcus sanguinis in a dual-species model","volume":"122","author":"Yoshida","year":"2014","journal-title":"APMIS"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Alves, L.A., Salvatierra, G.C., Freitas, V.A., H\u00f6fling, J.F., Bastos, D.C., Araujo, T.L.S., and Mattos-Graner, R.O. (2022). Diversity in Phenotypes Associated With Host Persistence and Systemic Virulence in Streptococcus sanguinis Strains. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.875581"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1016\/j.cell.2012.02.022","article-title":"The Amyloid State of Proteins in Human Diseases","volume":"148","author":"Eisenberg","year":"2012","journal-title":"Cell"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.tim.2020.09.005","article-title":"Two Decades of Studying Functional Amyloids in Microorganisms","volume":"29","author":"Levkovich","year":"2021","journal-title":"Trends Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e00062-20","DOI":"10.1128\/MMBR.00062-20","article-title":"Emerging Roles of Functional Bacterial Amyloids in Gene Regulation, Toxicity, and Immunomodulation","volume":"85","author":"Salinas","year":"2021","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_21","unstructured":"Mikhail Belousov Prokaryotic Amyloids in Interspecies Interactions (2023, October 04). Encyclopedia. Available online: https:\/\/encyclopedia.pub\/entry\/2620."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e00028-18","DOI":"10.1128\/MMBR.00028-18","article-title":"Curli-Containing Enteric Biofilms Inside and Out: Matrix Composition, Immune Recognition, and Disease Implications","volume":"82","author":"Tursi","year":"2018","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2903","DOI":"10.1099\/mic.0.060855-0","article-title":"Functional amyloid formation by Streptococcus mutans","volume":"158","author":"Oli","year":"2012","journal-title":"Microbiology"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/s13568-019-0753-1","article-title":"Characteristics and influencing factors of amyloid fibers in S. mutans biofilm","volume":"9","author":"Chen","year":"2019","journal-title":"AMB Express"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"di Cologna, N.M., Samaddar, S., Valle, C.A., Vargas, J., Aviles-Reyes, A., Morales, J., Ganguly, T., Pileggi, R., Brady, L.J., and Lemos, J.A. (2021). Amyloid Aggregation of Streptococcus mutans Cnm Influences Its Collagen-Binding Activity. Appl. Environ. Microbiol., 87.","DOI":"10.1128\/AEM.01149-21"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3657","DOI":"10.1016\/j.jmb.2018.07.010","article-title":"The Role of Functional Amyloids in Bacterial Virulence","volume":"430","author":"Reiter","year":"2018","journal-title":"J. Mol. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"5138","DOI":"10.1038\/s41598-020-62115-7","article-title":"Enhanced purification coupled with biophysical analyses shows cross-\u03b2 structure as a core building block for Streptococcus mutans functional amyloids","volume":"10","author":"Ocampo","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1099\/mic.0.000443","article-title":"Functional amyloids in Streptococcus mutans, their use as targets of biofilm inhibition and initial characterization of SMU_63c","volume":"163","author":"Besingi","year":"2017","journal-title":"Microbiology"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Biesecker, S.G., Nicastro, L.K., Wilson, R.P., and T\u00fckel, \u00c7. (2018). The Functional Amyloid Curli Protects Escherichia coli against Complement-Mediated Bactericidal Activity. Biomolecules, 8.","DOI":"10.3390\/biom8010005"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1714","DOI":"10.1101\/gad.264303","article-title":"A Novel Class of Secreted Hydrophobic Proteins Is Involved in Aerial Hyphae Formation in Streptomyces coelicolor by Forming Amyloid-like Fibrils","volume":"17","author":"Claessen","year":"2003","journal-title":"Genes Dev."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.jsb.2013.08.013","article-title":"Chaplins of Streptomyces coelicolor self-assemble into two distinct functional amyloids","volume":"184","author":"Bokhove","year":"2013","journal-title":"J. Struct. Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e00703-18","DOI":"10.1128\/IAI.00703-18","article-title":"Genomic, Phenotypic, and Virulence Analysis of Streptococcus sanguinis Oral and Infective-Endocarditis Isolates","volume":"87","author":"Baker","year":"2019","journal-title":"Infect. Immun."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1111\/j.1365-2958.2005.05020.x","article-title":"A major protein component of the Bacillus subtilis biofilm matrix","volume":"59","author":"Branda","year":"2006","journal-title":"Mol. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"5540","DOI":"10.1128\/JB.00946-13","article-title":"Cellulose as an Architectural Element in Spatially Structured Escherichia coli Biofilms","volume":"195","author":"Serra","year":"2013","journal-title":"J. Bacteriol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"12538","DOI":"10.1074\/jbc.R115.707547","article-title":"What\u2019s on the Outside Matters: The Role of the Extracellular Polymeric Substance of Gram-Negative Biofilms in Evading Host Immunity and as a Target for Therapeutic Intervention","volume":"291","author":"Gunn","year":"2016","journal-title":"J. Biol. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/s00294-015-0527-5","article-title":"Emerging interactions between matrix components during biofilm development","volume":"62","author":"Payne","year":"2016","journal-title":"Curr. Genet."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2551","DOI":"10.1128\/IAI.00338-08","article-title":"Identification of Streptococcus sanguinis Genes Required for Biofilm Formation and Examination of Their Role in Endocarditis Virulence","volume":"76","author":"Ge","year":"2008","journal-title":"Infect. Immun."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/microbiolspec.GPP3-0051-2018","article-title":"The Biology of Streptococcus mutans","volume":"7","author":"Lemos","year":"2019","journal-title":"Microbiol Spectr."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"878869","DOI":"10.3389\/fnins.2022.878869","article-title":"General Principles Underpinning Amyloid Structure","volume":"16","author":"Taylor","year":"2022","journal-title":"Front. Neurosci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1016\/j.bbamcr.2013.09.010","article-title":"Curli biogenesis: Order out of disorder","volume":"1843","author":"Evans","year":"2014","journal-title":"Biochim. Biophys. Acta BBA Mol. Cell Res."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Yarmola, E., Yarmola, E., Ishkov, I.P., Ishkov, I.P., di Cologna, N.M., di Cologna, N.M., Menashe, M., Menashe, M., Whitener, R.L., and Whitener, R.L. (2022). Amyloid Aggregates Are Localized to the Nonadherent Detached Fraction of Aging Streptococcus mutans Biofilms. Microbiol. Spectr., 10.","DOI":"10.1128\/spectrum.01661-22"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"3773","DOI":"10.1128\/IAI.73.6.3773-3777.2005","article-title":"Involvement of Sortase Anchoring of Cell Wall Proteins in Biofilm Formation by Streptococcus mutans","volume":"73","author":"Voronejskaia","year":"2005","journal-title":"Infect. Immun."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"4414","DOI":"10.1021\/acsbiomaterials.0c00188","article-title":"Green Tea Polyphenol Microparticles Based on the Oxidative Coupling of EGCG Inhibit Amyloid Aggregation\/Cytotoxicity and Serve as a Platform for Drug Delivery","volume":"6","author":"Fernandes","year":"2020","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"26540","DOI":"10.1074\/jbc.M116.739953","article-title":"Epigallocatechin Gallate Remodels Overexpressed Functional Amyloids in Pseudomonas aeruginosa and Increases Biofilm Susceptibility to Antibiotic Treatment","volume":"291","author":"Stenvang","year":"2016","journal-title":"J. Biol. Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"9370","DOI":"10.1039\/D0FO02054K","article-title":"Antibacterial green tea catechins from a molecular perspective: Mechanisms of action and structure\u2013activity relationships","volume":"11","author":"Renzetti","year":"2020","journal-title":"Food Funct."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Matilla-Cuenca, L., Taglialegna, A., Gil, C., Toledo-Arana, A., Lasa, I., and Valle, J. (2022). Bacterial biofilm functionalization through Bap amyloid engineering. NPJ Biofilms Microbiomes, 8.","DOI":"10.1038\/s41522-022-00324-w"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"718188","DOI":"10.3389\/fnins.2021.718188","article-title":"Green Tea Polyphenol Epigallocatechin-Gallate in Amyloid Aggregation and Neurodegenerative Diseases","volume":"15","author":"Fernandes","year":"2021","journal-title":"Front. Neurosci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1084\/jem.154.4.1134","article-title":"Fibronectin and C4-binding protein are selectively bound by aggregated amyloid P component","volume":"154","author":"DeBeer","year":"1981","journal-title":"J. Exp. Med."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1038\/338652a0","article-title":"Fibronectin binding mediated by a novel class of surface organelles on Escherichia coll","volume":"338","author":"Jonsson","year":"1989","journal-title":"Nature"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"10150","DOI":"10.1073\/pnas.90.21.10150","article-title":"\u03b2-Amyloid precursor protein binds to the neurite-promoting IKVAV site of laminin","volume":"90","author":"Kibbey","year":"1993","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1111\/j.1365-2958.1994.tb02179.x","article-title":"Plasminogen, absorbed by Escherichia coli expressing curli or by Salmonella enteritidis expressing thin aggregative fimbriae, can be activated by simultaneously captured tissue-type plasminogen activator (t-PA)","volume":"14","author":"Pohl","year":"1994","journal-title":"Mol. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/s13024-021-00465-0","article-title":"Extracellular protein components of amyloid plaques and their roles in Alzheimer\u2019s disease pathology","volume":"16","author":"Rahman","year":"2021","journal-title":"Mol. Neurodegener."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"4299","DOI":"10.1073\/pnas.92.10.4299","article-title":"Serum amyloid P component prevents proteolysis of the amyloid fibrils of Alzheimer disease and systemic amyloidosis","volume":"92","author":"Lovat","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Klotz, S.A., and Lipke, P.N. (2022). The Paradoxical Effects of Serum Amyloid-P Component on Disseminated Candidiasis. Pathogens, 11.","DOI":"10.3390\/pathogens11111304"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.ijcard.2015.03.179","article-title":"SAP: Structure, Function, and Its Roles in Immune-Related Diseases","volume":"187","author":"Xi","year":"2015","journal-title":"Int. J. Cardiol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Yuste, J., Botto, M., Bottoms, S.E., and Brown, J.S. (2007). Serum Amyloid P Aids Complement-Mediated Immunity to Streptococcus pneumoniae. PLoS Pathog., 3.","DOI":"10.1371\/journal.ppat.0030120"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"3739","DOI":"10.1038\/s41467-021-24021-y","article-title":"Serum Amyloid P Component Is an Essential Element of Resistance against Aspergillus fumigatus","volume":"12","author":"Doni","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2239519","DOI":"10.1080\/21505594.2023.2239519","article-title":"PepO and CppA modulate Streptococcus sanguinis susceptibility to complement immunity and virulence","volume":"14","author":"Alves","year":"2023","journal-title":"Virulence"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1080\/21505594.2020.1767377","article-title":"PepO is a target of the two-component systems VicRK and CovR required for systemic virulence of Streptococcus mutans","volume":"11","author":"Alves","year":"2020","journal-title":"Virulence"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"4244","DOI":"10.1074\/jbc.M116.749275","article-title":"Streptococcus pyogenes Endopeptidase O Contributes to Evasion from Complement-Mediated Bacteriolysis via Binding to Human Complement Factor C1q","volume":"292","author":"Sumitomo","year":"2017","journal-title":"J. Biol. Chem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.molimm.2013.05.018","article-title":"Streptococcus pneumoniae: Endopeptidase O (PepO): A Novel C1q Binding Complement Inhibitor","volume":"56","author":"Agarwal","year":"2013","journal-title":"Mol. Immunol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1038\/aps.2017.28","article-title":"Amyloid beta: Structure, Biology and Structure-Based Therapeutic Development","volume":"38","author":"Chen","year":"2017","journal-title":"Acta Pharmacol. Sin."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2791","DOI":"10.1016\/j.micinf.2006.08.010","article-title":"Role of Streptococcus sanguinis Sortase A in Bacterial Colonization","volume":"8","author":"Yamaguchi","year":"2006","journal-title":"Microbes Infect."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1038\/s41423-018-0024-0","article-title":"NETosis, Complement, and Coagulation: A Triangular Relationship","volume":"16","author":"Boelens","year":"2019","journal-title":"Cell. Mol. Immunol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"e2122716119","DOI":"10.1073\/pnas.2122716119","article-title":"Neutrophils Activated by Membrane Attack Complexes Increase the Permeability of Melanoma Blood Vessels","volume":"119","author":"Liu","year":"2022","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"781273","DOI":"10.3389\/fimmu.2022.781273","article-title":"Reduced Neutrophil Extracellular Trap Formation During Ischemia Reperfusion Injury in C3 KO Mice: C3 Requirement for NETs Release","volume":"13","author":"Wu","year":"2022","journal-title":"Front. Immunol."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Raz, A., Tanasescu, A.-M., Zhao, A.M., Serrano, A., Alston, T., Sol, A., Bachrach, G., and Fischetti, V.A. (2015). Streptococcus pyogenes Sortase Mutants Are Highly Susceptible to Killing by Host Factors Due to Aberrant Envelope Physiology. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0140784"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1099\/00207713-39-4-471","article-title":"Taxonomic Study of Viridans Streptococci: Description of Streptococcus gordonii Sp. Nov. and Emended Descriptions of Streptococcus sanguis (White and Niven 1946), Streptococcus oralis (Bridge and Sneath 1982), and Streptococcus mitis (Andrewes and Horder 1906)","volume":"39","author":"Kilian","year":"1989","journal-title":"Int. J. Syst. Bacteriol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"2277","DOI":"10.1128\/IAI.00767-10","article-title":"The Collagen-Binding Protein Cnm Is Required for Streptococcus mutans Adherence to and Intracellular Invasion of Human Coronary Artery Endothelial Cells","volume":"79","author":"Abranches","year":"2011","journal-title":"Infect. Immun."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"6521","DOI":"10.1128\/JB.00825-07","article-title":"Regulation of gbpC Expression in Streptococcus mutans","volume":"189","author":"Biswas","year":"2007","journal-title":"J. Bacteriol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"e00942-17","DOI":"10.1128\/IAI.00942-17","article-title":"Novel Two-Component System of Streptococcus sanguinis Affecting Functions Associated with Viability in Saliva and Biofilm Formation","volume":"86","author":"Camargo","year":"2018","journal-title":"Infect. Immun."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/0378-1119(82)90025-7","article-title":"A Cloning Vector Able to Replicate in Escherichia coli and Streptococcus sanguis","volume":"19","author":"Macrina","year":"1982","journal-title":"Gene"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1128\/aem.57.4.1194-1201.1991","article-title":"Improved Electroporation and Cloning Vector System for Gram-Positive Bacteria","volume":"57","author":"Dunny","year":"1991","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Harth-Chu, E.N., Alves, L.A., Theobaldo, J.D., Salom\u00e3o, M.F., H\u00f6fling, J.F., King, W.F., Smith, D.J., and Mattos-Graner, R.O. (2019). PcsB Expression Diversity Influences on Streptococcus mitis Phenotypes Associated with Host Persistence and Virulence. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.02567"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1111\/j.1601-0825.2011.01896.x","article-title":"Influence of VicRK and CovR on the Interactions of Streptococcus mutans with Phagocytes","volume":"18","author":"Negrini","year":"2012","journal-title":"Oral Dis."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/21\/15686\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:13:14Z","timestamp":1760130794000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/21\/15686"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,28]]},"references-count":75,"journal-issue":{"issue":"21","published-online":{"date-parts":[[2023,11]]}},"alternative-id":["ijms242115686"],"URL":"https:\/\/doi.org\/10.3390\/ijms242115686","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,28]]}}}