{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T14:17:32Z","timestamp":1765808252655,"version":"build-2065373602"},"reference-count":39,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T00:00:00Z","timestamp":1698451200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Instituto de Salud Carlos III","award":["PI18\/01582","PI21\/01282","PID2019-106099RB-C42","PROMETEO\/2020\/069","OGMIOS\/INNEST\/2021\/55","TV3-736\/C\/2020"],"award-info":[{"award-number":["PI18\/01582","PI21\/01282","PID2019-106099RB-C42","PROMETEO\/2020\/069","OGMIOS\/INNEST\/2021\/55","TV3-736\/C\/2020"]}]},{"DOI":"10.13039\/501100004837","name":"Ministry of Economy and Competitiveness of the Spanish Government","doi-asserted-by":"publisher","award":["PI18\/01582","PI21\/01282","PID2019-106099RB-C42","PROMETEO\/2020\/069","OGMIOS\/INNEST\/2021\/55","TV3-736\/C\/2020"],"award-info":[{"award-number":["PI18\/01582","PI21\/01282","PID2019-106099RB-C42","PROMETEO\/2020\/069","OGMIOS\/INNEST\/2021\/55","TV3-736\/C\/2020"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003359","name":"Comunitat Valenciana (Spain)","doi-asserted-by":"publisher","award":["PI18\/01582","PI21\/01282","PID2019-106099RB-C42","PROMETEO\/2020\/069","OGMIOS\/INNEST\/2021\/55","TV3-736\/C\/2020"],"award-info":[{"award-number":["PI18\/01582","PI21\/01282","PID2019-106099RB-C42","PROMETEO\/2020\/069","OGMIOS\/INNEST\/2021\/55","TV3-736\/C\/2020"]}],"id":[{"id":"10.13039\/501100003359","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Agencia Valenciana de Innovaci\u00f3n","award":["PI18\/01582","PI21\/01282","PID2019-106099RB-C42","PROMETEO\/2020\/069","OGMIOS\/INNEST\/2021\/55","TV3-736\/C\/2020"],"award-info":[{"award-number":["PI18\/01582","PI21\/01282","PID2019-106099RB-C42","PROMETEO\/2020\/069","OGMIOS\/INNEST\/2021\/55","TV3-736\/C\/2020"]}]},{"name":"La Marat\u00f3","award":["PI18\/01582","PI21\/01282","PID2019-106099RB-C42","PROMETEO\/2020\/069","OGMIOS\/INNEST\/2021\/55","TV3-736\/C\/2020"],"award-info":[{"award-number":["PI18\/01582","PI21\/01282","PID2019-106099RB-C42","PROMETEO\/2020\/069","OGMIOS\/INNEST\/2021\/55","TV3-736\/C\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Advances in regenerative medicine have enabled the search for new solutions to current health problems in so far unexplored fields. Thus, we focused on cadaveric subcutaneous fat as a promising source of adipose-derived stem cells (ADSCs) that have potential to differentiate into different cell lines. With this aim, we isolated and characterized ADSCs from cadaveric samples with a postmortem interval ranging from 30 to 55 h and evaluated their ability to differentiate into chondrocytes or osteocytes. A commercial ADSC line was used as reference. Morphological and protein expression analyses were used to confirm the final stage of differentiation. Eight out of fourteen samples from patients were suitable to complete the whole protocol. Cadaveric ADSCs exhibited features of stem cells based upon several markers: CD29 (84.49 \u00b1 14.07%), CD105 (94.38 \u00b1 2.09%), and CD44 (99.77 \u00b1 0.32%). The multiparametric assessment of differentiation confirmed the generation of stable lines of chondrocytes and osteocytes. In conclusion, we provide evidence supporting the feasibility of obtaining viable postmortem human subcutaneous fat ADSCs with potential application in tissue engineering and research fields.<\/jats:p>","DOI":"10.3390\/ijms242115696","type":"journal-article","created":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T09:27:28Z","timestamp":1698658048000},"page":"15696","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Cadaveric Adipose-Derived Stem Cells for Regenerative Medicine and Research"],"prefix":"10.3390","volume":"24","author":[{"given":"Lara","family":"Mili\u00e1n","sequence":"first","affiliation":[{"name":"Department of Pathology, Faculty of Medicine and Dentistry, Universitat de Val\u00e8ncia, 46010 Valencia, Spain"},{"name":"INCLIVA Biomedical Research Institute, 46010 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pilar","family":"Molina","sequence":"additional","affiliation":[{"name":"Department of Pathology, Instituto de Medicina Legal y Ciencias Forenses, 46010 Valencia, Spain"},{"name":"CAFAMUSME Research Group, Instituto de Investigaci\u00f3n Sanitaria La Fe, 46026 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Oliver-Ferr\u00e1ndiz","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Medicine and Dentistry, Universitat de Val\u00e8ncia, 46010 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Fern\u00e1ndez-Sellers","sequence":"additional","affiliation":[{"name":"Department of Pathology, Instituto de Medicina Legal y Ciencias Forenses, 46010 Valencia, Spain"},{"name":"CAFAMUSME Research Group, Instituto de Investigaci\u00f3n Sanitaria La Fe, 46026 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Monz\u00f3","sequence":"additional","affiliation":[{"name":"Department of Pathology, Instituto de Medicina Legal y Ciencias Forenses, 46010 Valencia, Spain"},{"name":"CAFAMUSME Research Group, Instituto de Investigaci\u00f3n Sanitaria La Fe, 46026 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"S\u00e1nchez-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"CAFAMUSME Research Group, Instituto de Investigaci\u00f3n Sanitaria La Fe, 46026 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aitana","family":"Braza-Boils","sequence":"additional","affiliation":[{"name":"CAFAMUSME Research Group, Instituto de Investigaci\u00f3n Sanitaria La Fe, 46026 Valencia, Spain"},{"name":"CIBERCV, Center for Biomedical Network Research on Cardiovascular Diseases, 28015 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1506-7983","authenticated-orcid":false,"given":"Manuel","family":"Mata","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Medicine and Dentistry, Universitat de Val\u00e8ncia, 46010 Valencia, Spain"},{"name":"INCLIVA Biomedical Research Institute, 46010 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3608-4210","authenticated-orcid":false,"given":"Esther","family":"Zorio","sequence":"additional","affiliation":[{"name":"CAFAMUSME Research Group, Instituto de Investigaci\u00f3n Sanitaria La Fe, 46026 Valencia, Spain"},{"name":"CIBERCV, Center for Biomedical Network Research on Cardiovascular Diseases, 28015 Madrid, Spain"},{"name":"Inherited Cardiac Diseases Unit, Cardiology Department, Hospital Universitario y Polit\u00e9cnico La Fe, 46026 Valencia, Spain"},{"name":"Department of Medicine, Faculty of Medicine and Dentistry, Universitat de Val\u00e8ncia, 46010 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1186\/s13287-019-1165-5","article-title":"Stem Cells: Past, Present, and Future","volume":"10","author":"Zakrzewski","year":"2019","journal-title":"Stem Cell Res. 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Cell Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.stem.2008.07.003","article-title":"A Perivascular Origin for Mesenchymal Stem Cells in Multiple Human Organs","volume":"3","author":"Crisan","year":"2008","journal-title":"Cell Stem Cell"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1089\/107632701300062859","article-title":"Multilineage Cells from Human Adipose Tissue: Implications for Cell-Based Therapies","volume":"7","author":"Zuk","year":"2001","journal-title":"Tissue Eng."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.ymeth.2008.03.006","article-title":"Adipose-Derived Stem Cells: Isolation, Expansion and Differentiation","volume":"45","author":"Bunnell","year":"2008","journal-title":"Methods"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3206807","DOI":"10.1155\/2016\/3206807","article-title":"Advances in Adipose-Derived Stem Cells Isolation, Characterization, and Application in Regenerative Tissue Engineering","volume":"2016","author":"Wankhade","year":"2016","journal-title":"Stem Cells Int."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1568","DOI":"10.1100\/tsw.2011.146","article-title":"Current Status of Human Adipose-Derived Stem Cells: Differentiation into Hepatocyte-like Cells","volume":"11","author":"Vanhaecke","year":"2011","journal-title":"ScientificWorldJournal"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1002\/wsbm.141","article-title":"Human Cardiomyogenesis and the Need for Systems Biology Analysis","volume":"3","author":"Young","year":"2011","journal-title":"Wiley Interdiscip. Rev. Syst. Biol. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"153","DOI":"10.2174\/157488810791268645","article-title":"Neural Differentiation and Therapeutic Potential of Adipose Tissue Derived Stem Cells","volume":"5","author":"Erba","year":"2010","journal-title":"Curr. Stem Cell Res. Ther."},{"key":"ref_12","first-page":"113","article-title":"Trans-Differentiation of the Adipose Tissue-Derived Stem Cells into Neuron-like Cells Expressing Neurotrophins by Selegiline","volume":"15","author":"Abdanipour","year":"2011","journal-title":"Iran. Biomed. J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.bbrc.2010.09.012","article-title":"Myocardial Regeneration Potential of Adipose Tissue-Derived Stem Cells","volume":"401","author":"Bai","year":"2010","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1825","DOI":"10.1016\/j.jvs.2015.09.054","article-title":"Coadministration of Adipose-Derived Stem Cells and Control-Released Basic Fibroblast Growth Factor Facilitates Angiogenesis in a Murine Ischemic Hind Limb Model","volume":"64","author":"Tobita","year":"2016","journal-title":"J. Vasc. Surg."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/B978-0-12-410499-0.00001-0","article-title":"Tissue Engineering and Regenerative Medicine: Past, Present, and Future","volume":"108","author":"Salgado","year":"2013","journal-title":"Int. Rev. Neurobiol."},{"key":"ref_16","first-page":"31","article-title":"Adipose-Derived Stem Cells in Orthopaedic Pathologies","volume":"124","author":"Usuelli","year":"2017","journal-title":"Br. Med. Bull."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"dmm039347","DOI":"10.1242\/dmm.039347","article-title":"Disease Modelling in Human Organoids","volume":"12","author":"Lancaster","year":"2019","journal-title":"Dis. Model. Mech."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"eaam8645","DOI":"10.1126\/scitranslmed.aam8645","article-title":"Engineering Precision Biomaterials for Personalized Medicine","volume":"10","author":"Aguado","year":"2018","journal-title":"Sci. Transl. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"798161","DOI":"10.3389\/fgene.2021.798161","article-title":"Cadaveric Stem Cells: Their Research Potential and Limitations","volume":"12","author":"Tomsia","year":"2021","journal-title":"Front. Genet."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1016\/j.transproceed.2013.11.110","article-title":"Cardiac Mesenchymal Progenitors from Postmortem Cardiac Tissues Retained Cellular Characterization","volume":"46","author":"Kami","year":"2014","journal-title":"Transplant. Proc."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1186\/s13287-015-0250-7","article-title":"Tissues from Equine Cadaver Ligaments up to 72 Hours of Post-Mortem: A Promising Reservoir of Stem Cells","volume":"6","author":"Gabriel","year":"2015","journal-title":"Stem Cell Res. Ther."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1038\/ncomms1890","article-title":"Skeletal Muscle Stem Cells Adopt a Dormant Cell State Post Mortem and Retain Regenerative Capacity","volume":"3","author":"Latil","year":"2012","journal-title":"Nat. Commun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1007\/s00414-005-0037-y","article-title":"The Human Brain and Its Neural Stem Cells Postmortem: From Dead Brains to Live Therapy","volume":"120","author":"Feldmann","year":"2006","journal-title":"Int. J. Leg. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1002\/jnr.10854","article-title":"Isolation and Characterization of Neural Progenitor Cells from Post-Mortem Human Cortex","volume":"74","author":"Schwartz","year":"2003","journal-title":"J. Neurosci. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"5067","DOI":"10.1182\/blood-2010-08-304287","article-title":"CD146 Expression on Primary Nonhematopoietic Bone Marrow Stem Cells Is Correlated with in Situ Localization","volume":"117","author":"Tormin","year":"2011","journal-title":"Blood"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1111\/jcmm.12168","article-title":"CD146 Expression on Mesenchymal Stem Cells Is Associated with Their Vascular Smooth Muscle Commitment","volume":"18","author":"Espagnolle","year":"2014","journal-title":"J. Cell Mol. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"5105","DOI":"10.7150\/thno.33904","article-title":"Enrichment of CD146 (+) Adipose-Derived Stem Cells in Combination with Articular Cartilage Extracellular Matrix Scaffold Promotes Cartilage Regeneration","volume":"9","author":"Li","year":"2019","journal-title":"Theranostics"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1002\/stem.2560","article-title":"The Mesenchymal Precursor Cell Marker Antibody STRO-1 Binds to Cell Surface Heat Shock Cognate 70","volume":"35","author":"Fitter","year":"2017","journal-title":"Stem Cells"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2201","DOI":"10.1634\/stemcells.2008-0428","article-title":"Concise Review: Mesenchymal Stromal Cells: Potential for Cardiovascular Repair","volume":"26","author":"Psaltis","year":"2008","journal-title":"Stem Cells"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e5809","DOI":"10.7717\/peerj.5809","article-title":"Chondrogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells Induced by L-Ascorbic Acid and Platelet Rich Plasma on Silk Fibroin Scaffold","volume":"6","author":"Barlian","year":"2018","journal-title":"PeerJ"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Oliver-Ferr\u00e1ndiz, M., Mili\u00e1n, L., Sancho-Tello, M., Mart\u00edn de Llano, J.J., Gisbert Roca, F., Mart\u00ednez-Ramos, C., Carda, C., and Mata, M. (2021). Alginate-Agarose Hydrogels Improve the In Vitro Differentiation of Human Dental Pulp Stem Cells in Chondrocytes. A Histological Study. Biomedicines, 9.","DOI":"10.3390\/biomedicines9070834"},{"key":"ref_32","first-page":"7843798","article-title":"Chondrogenic Potential of Human Dental Pulp Stem Cells Cultured as Microtissues","volume":"2021","author":"Oliver","year":"2021","journal-title":"Stem Cells Int."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2041731417697545","DOI":"10.1177\/2041731417697545","article-title":"Human Platelet-Rich Plasma Improves the Nesting and Differentiation of Human Chondrocytes Cultured in Stabilized Porous Chitosan Scaffolds","volume":"8","author":"Martorell","year":"2017","journal-title":"J. Tissue Eng."},{"key":"ref_34","first-page":"4910399","article-title":"BMP-2 Enhances Osteogenic Differentiation of Human Adipose-Derived and Dental Pulp Stem Cells in 2D and 3D In Vitro Models","volume":"2022","author":"Milian","year":"2022","journal-title":"Stem Cells Int."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1186\/s13287-018-0942-x","article-title":"In Vitro Osteoblastic Differentiation of Mesenchymal Stem Cells Generates Cell Layers with Distinct Properties","volume":"9","author":"Hanna","year":"2018","journal-title":"Stem Cell Res. Ther."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"115971","DOI":"10.1016\/j.bone.2021.115971","article-title":"The Cytoskeleton and Connected Elements in Bone Cell Mechano-Transduction","volume":"149","author":"Gould","year":"2021","journal-title":"Bone"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"36","DOI":"10.7150\/ijms.21666","article-title":"Ethical and Safety Issues of Stem Cell-Based Therapy","volume":"15","author":"Volarevic","year":"2018","journal-title":"Int. J. Med. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"7713","DOI":"10.18632\/oncotarget.3481","article-title":"Adipose-Derived Stem Cells Promote Tumor Initiation and Accelerate Tumor Growth by Interleukin-6 Production","volume":"6","author":"Wei","year":"2015","journal-title":"Oncotarget"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e0228909","DOI":"10.1371\/journal.pone.0228909","article-title":"Cannabinoid Receptor Expression in Non-Small Cell Lung Cancer. Effectiveness of Tetrahydrocannabinol and Cannabidiol Inhibiting Cell Proliferation and Epithelial-Mesenchymal Transition in Vitro","volume":"15","author":"Milian","year":"2020","journal-title":"PLoS ONE"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/21\/15696\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:13:21Z","timestamp":1760130801000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/21\/15696"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,28]]},"references-count":39,"journal-issue":{"issue":"21","published-online":{"date-parts":[[2023,11]]}},"alternative-id":["ijms242115696"],"URL":"https:\/\/doi.org\/10.3390\/ijms242115696","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2023,10,28]]}}}