{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T17:59:48Z","timestamp":1780336788940,"version":"3.54.1"},"reference-count":38,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:00:00Z","timestamp":1698710400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1555487"],"award-info":[{"award-number":["1555487"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["T32GM145441"],"award-info":[{"award-number":["T32GM145441"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Linda and Rich Maxon undergraduate research award","award":["1555487"],"award-info":[{"award-number":["1555487"]}]},{"name":"Linda and Rich Maxon undergraduate research award","award":["T32GM145441"],"award-info":[{"award-number":["T32GM145441"]}]},{"name":"University of Iowa Graduate College Summer Fellowship","award":["1555487"],"award-info":[{"award-number":["1555487"]}]},{"name":"University of Iowa Graduate College Summer Fellowship","award":["T32GM145441"],"award-info":[{"award-number":["T32GM145441"]}]},{"name":"Lulu Merle Johnson Fellowship","award":["1555487"],"award-info":[{"award-number":["1555487"]}]},{"name":"Lulu Merle Johnson Fellowship","award":["T32GM145441"],"award-info":[{"award-number":["T32GM145441"]}]},{"name":"NIH Predoctoral Training","award":["1555487"],"award-info":[{"award-number":["1555487"]}]},{"name":"NIH Predoctoral Training","award":["T32GM145441"],"award-info":[{"award-number":["T32GM145441"]}]},{"name":"Avis Cone Undergraduate Summer Fellowship","award":["1555487"],"award-info":[{"award-number":["1555487"]}]},{"name":"Avis Cone Undergraduate Summer Fellowship","award":["T32GM145441"],"award-info":[{"award-number":["T32GM145441"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Growth and development of the Ceratopteris hermaphroditic gametophytes are dependent on cell proliferation in the marginal meristem, which when destroyed will regenerate at a new location on the body margin. We established a laser ablation method to destroy a single initial cell in the meristem. Ablation caused the cessation of cell proliferation accompanied by the disappearance of the expression of an auxin synthesis gene (CrTAA2) and a cell proliferation marker gene (CrWOXB). New meristem regeneration occurred within a predictable distance from the original two days post-ablation, signified by cell proliferation and the expression of CrTAA2. Treatment with the naturally occurring auxin indole-3-acetic acid (IAA), synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D), or the transport inhibitor naphthylphthalamic acid (NPA) altered positioning of the original marginal meristem toward the apex of the gametophyte. IAA altered positioning of the regenerated meristem after damaging the original meristem. A model of auxin involvement in the positioning of the marginal meristem in Ceratopteris is presented to encompass these results.<\/jats:p>","DOI":"10.3390\/ijms242115832","type":"journal-article","created":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T11:16:27Z","timestamp":1698750987000},"page":"15832","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Auxin Involvement in Ceratopteris Gametophyte Meristem Regeneration"],"prefix":"10.3390","volume":"24","author":[{"given":"Kelley A.","family":"Withers","sequence":"first","affiliation":[{"name":"Department of Biology, University of Iowa, Iowa City, IA 52242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Athena","family":"Kvamme","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Iowa, Iowa City, IA 52242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher E.","family":"Youngstrom","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Iowa, Iowa City, IA 52242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebekah M.","family":"Yarvis","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Iowa, Iowa City, IA 52242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel","family":"Orpano","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Iowa, Iowa City, IA 52242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel P.","family":"Simons","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Iowa, Iowa City, IA 52242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin E.","family":"Irish","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Iowa, Iowa City, IA 52242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi-Lien","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Iowa, Iowa City, IA 52242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1093\/plphys\/kiac313","article-title":"How was apical growth regulated in the ancestral land plant? 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