{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T07:09:44Z","timestamp":1787987384234,"version":"build-2784847793"},"reference-count":48,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:00:00Z","timestamp":1698710400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["91856126"],"award-info":[{"award-number":["91856126"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2020C02035"],"award-info":[{"award-number":["2020C02035"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["215-432000282"],"award-info":[{"award-number":["215-432000282"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["215-432094250"],"award-info":[{"award-number":["215-432094250"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Science Technology Department of Zhejiang Province","award":["91856126"],"award-info":[{"award-number":["91856126"]}]},{"name":"Science Technology Department of Zhejiang Province","award":["2020C02035"],"award-info":[{"award-number":["2020C02035"]}]},{"name":"Science Technology Department of Zhejiang Province","award":["215-432000282"],"award-info":[{"award-number":["215-432000282"]}]},{"name":"Science Technology Department of Zhejiang Province","award":["215-432094250"],"award-info":[{"award-number":["215-432094250"]}]},{"name":"Scientific Research Grant of Ningbo University","award":["91856126"],"award-info":[{"award-number":["91856126"]}]},{"name":"Scientific Research Grant of Ningbo University","award":["2020C02035"],"award-info":[{"award-number":["2020C02035"]}]},{"name":"Scientific Research Grant of Ningbo University","award":["215-432000282"],"award-info":[{"award-number":["215-432000282"]}]},{"name":"Scientific Research Grant of Ningbo University","award":["215-432094250"],"award-info":[{"award-number":["215-432094250"]}]},{"name":"Ningbo Top Talent Project","award":["91856126"],"award-info":[{"award-number":["91856126"]}]},{"name":"Ningbo Top Talent Project","award":["2020C02035"],"award-info":[{"award-number":["2020C02035"]}]},{"name":"Ningbo Top Talent Project","award":["215-432000282"],"award-info":[{"award-number":["215-432000282"]}]},{"name":"Ningbo Top Talent Project","award":["215-432094250"],"award-info":[{"award-number":["215-432094250"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Plant proteins are a good source of active peptides, which can exert physiological effects on the body. Predicting the possible activity of plant proteins and obtaining active peptides with oral potential are challenging. In this study, the potential activity of peptides from Zizyphus jujuba proteins after in silico simulated gastrointestinal digestion was predicted using the BIOPEP-UWM\u2122 database. The ACE-inhibitory activity needs to be further investigated. The actual peptides in mouse intestines after the oral administration of Zizyphus jujuba protein were collected and analyzed, 113 Zizyphus jujuba peptides were identified, and 3D-QSAR models of the ACE-inhibitory activity were created and validated using a training set (34 peptides) and a test set (12 peptides). Three peptides, RLPHV, TVKPGL and KALVAP, were screened using the 3D-QSAR model and were found to bind to the active sites of the ACE enzyme, and their IC50 values were determined. Their values were 6.01, 3.81, and 17.06 \u03bcM, respectively. The in vitro digestion stabilities of the RLPHV, TVKPGL, and KALVAP peptides were 82%, 90%, and 78%. This article provides an integrated method for studying bioactive peptides derived from plant proteins.<\/jats:p>","DOI":"10.3390\/ijms242115848","type":"journal-article","created":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T14:01:49Z","timestamp":1698760909000},"page":"15848","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Screening of Oral Potential Angiotensin-Converting Enzyme Inhibitory Peptides from Zizyphus jujuba Proteins Based on Gastrointestinal Digestion In Vivo"],"prefix":"10.3390","volume":"24","author":[{"given":"Xinchang","family":"Gao","sequence":"first","affiliation":[{"name":"Institute of Drug Discovery Technology, Ningbo University, Ningbo 315211, China"},{"name":"Department of Chemistry, Tsinghua University, Beijing 100084, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Chinese Academy of Fishery Sciences, Beijing 100141, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5625-5358","authenticated-orcid":false,"given":"Ning","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Drug Discovery Technology, Ningbo University, Ningbo 315211, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8891-0655","authenticated-orcid":false,"given":"Jin-Ming","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Tsinghua University, Beijing 100084, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6853-4699","authenticated-orcid":false,"given":"Yali","family":"Dang","sequence":"additional","affiliation":[{"name":"College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufen","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Drug Discovery Technology, Ningbo University, Ningbo 315211, China"},{"name":"Department of Chemistry, Tsinghua University, Beijing 100084, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.foodchem.2018.03.052","article-title":"Immunomodulatory acidic polysaccharides from Zizyphus jujuba cv. 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