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Its activation followed by the phosphorylation of downstream effectors causes hyperexcitability of primary sensory neurons in the dorsal root ganglion. We investigated whether a single injection of rAAV-shmTOR would effectively downregulate both complexes of mTOR in the long-term and glial activation as well. Male SD rats were categorized into shmTOR (n = 29), shCON (n = 23), SNI (n = 13), and Normal (n = 8) groups. Treatment groups were injected with rAAV-shmTOR or rAAV-shCON, respectively. DRG tissues and sciatic nerve were harvested for Western blot and immunohistochemical analyses. Peripheral sensitization was gradually attenuated in the shmTOR group, and it reached a peak on PID 21. Western blot analysis showed that both p-mTORC1 and p-mTORC2 were downregulated in the DRG compared to shCON and SNI groups. We also found decreased expression of phosphorylated p38 and microglial activation in the DRG. We first attempted a therapeutic strategy for neuropathic pain with a low dose of AAV injection by interfering with the mTOR signaling pathway, suggesting its potential application in pain treatment.<\/jats:p>","DOI":"10.3390\/ijms242115918","type":"journal-article","created":{"date-parts":[[2023,11,3]],"date-time":"2023-11-03T02:41:56Z","timestamp":1698979316000},"page":"15918","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Single Injection of rAAV-shmTOR in Peripheral Nerve Persistently Attenuates Nerve Injury-Induced Mechanical Allodynia"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7896-1640","authenticated-orcid":false,"given":"Minkyung","family":"Park","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Yonsei University College of Medicine, Seoul 03722, Republic of Korea"},{"name":"Brain Korea 21 PLUS Project for Medical Science and Brain Research Institute, Yonsei University College of Medicine, Seoul 03722, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6771-8151","authenticated-orcid":false,"given":"Ha-Na","family":"Woo","sequence":"additional","affiliation":[{"name":"Department of Biochemistry & Molecular Biology, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea"},{"name":"Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2115-7894","authenticated-orcid":false,"given":"Chin Su","family":"Koh","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Yonsei University College of Medicine, Seoul 03722, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4116-8980","authenticated-orcid":false,"given":"Heesue","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Yonsei University College of Medicine, Seoul 03722, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea"},{"name":"Department of Microbiology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul 05505, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keerang","family":"Park","sequence":"additional","affiliation":[{"name":"Cedmogen Co., Ltd., Cheongju 28644, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2717-0101","authenticated-orcid":false,"given":"Jin Woo","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Yonsei University College of Medicine, Seoul 03722, Republic of Korea"},{"name":"Brain Korea 21 PLUS Project for Medical Science and Brain Research Institute, Yonsei University College of Medicine, Seoul 03722, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heuiran","family":"Lee","sequence":"additional","affiliation":[{"name":"Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea"},{"name":"Department of Microbiology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul 05505, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8289-564X","authenticated-orcid":false,"given":"Hyun Ho","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Yonsei University College of Medicine, Seoul 03722, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.neuron.2006.09.021","article-title":"Mechanisms of neuropathic pain","volume":"52","author":"Campbell","year":"2006","journal-title":"Neuron"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S23","DOI":"10.1093\/pm\/pnz012","article-title":"Unique characteristics of the dorsal root ganglion as a target for neuromodulation","volume":"20","author":"Esposito","year":"2019","journal-title":"Pain Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1007\/978-1-60761-938-3_6","article-title":"The blood-nerve barrier: Structure and functional significance","volume":"686","author":"Weerasuriya","year":"2011","journal-title":"Methods Mol. 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