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A hybrid drug nanocarrier based on a PDA decorated with polyamidoamine (PAMAM) dendrimers G 3.0 (DG3) followed by a connection with glycol (PEG) moieties, folic acid (FA), and drug doxorubicin (DOX) was used for combined chemo- and photothermal therapy (CT-PTT) of liver cancer. Oxidative stress plays a crucial role in the development of cancer, and PDA seems to have the ability to both donate and accept electrons. We investigated oxidative stress in organs by evaluating oxidative stress markers in vivo. In the liver, the level of reduced glutathione (GSH) was lower and the level of Trolox equivalent antioxidant capacity (TEAC) was higher in the group receiving doxorubicin encapsulated in PDA nanoparticles with phototherapy (PDA@DG3@PEG@FA@DOX + PTT) compared to the control group. The concentration of thiobarbituric acid reactive substances (TBARS) in livers, was higher in the group receiving PDA coated with PAMAM dendrimers and functionalized with PEG and FA (PDA@DG3@PEG@FA) than in other groups. Markers in the brain also showed lower levels of GSH in the PDA@DG3@PEG@FA group than in the control group. Markers of oxidative stress indicated changes in the organs of animals receiving PDA nanoparticles with PAMAM dendrimers functionalized with FA in CT-PTT of liver cancer under in vivo conditions. Our work will provide insights into oxidative stress, which can be an indicator of the toxic potential of PDA nanoparticles and provide new strategies to improve existing therapies.<\/jats:p>","DOI":"10.3390\/ijms242316565","type":"journal-article","created":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T10:24:13Z","timestamp":1700562253000},"page":"16565","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Oxidative Stress in Xenograft Mouse Model Exposed to Dendrimers Decorated Polydopamine Nanoparticles and Targeted Chemo- and Photothermal Therapy"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5792-4659","authenticated-orcid":false,"given":"Marta","family":"Witkowska","sequence":"first","affiliation":[{"name":"Laboratory of Environmental Research, Department of Toxicology, Poznan University of Medical Sciences, Rokietnicka 3, 60-806 Pozna\u0144, Poland"},{"name":"Faculty of Chemistry, Adam Mickiewicz University, Uniwersytetu Pozna\u0144skiego 8, 61-614 Pozna\u0144, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3687-911X","authenticated-orcid":false,"given":"Rados\u0142aw","family":"Mr\u00f3wczy\u0144ski","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Adam Mickiewicz University, Uniwersytetu Pozna\u0144skiego 8, 61-614 Pozna\u0144, Poland"},{"name":"Centre for Advanced Technologies, Adam Mickiewicz University, Uniwersytetu Pozna\u0144skiego 10, 61-614 Pozna\u0144, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bartosz","family":"Grze\u015bkowiak","sequence":"additional","affiliation":[{"name":"NanoBioMedical Centre, Adam Mickiewicz University in Poznan, Uniwersytetu Pozna\u0144skiego 3, 61-614 Pozna\u0144, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0751-4867","authenticated-orcid":false,"given":"Izabela","family":"Miechowicz","sequence":"additional","affiliation":[{"name":"Department of Computer Science and Statistics, Poznan University of Medical Sciences, 60-806 Pozna\u0144, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2689-3009","authenticated-orcid":false,"given":"Ewa","family":"Florek","sequence":"additional","affiliation":[{"name":"Laboratory of Environmental Research, Department of Toxicology, Poznan University of Medical Sciences, Rokietnicka 3, 60-806 Pozna\u0144, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1776","DOI":"10.1039\/C8BM01642A","article-title":"A Polydopamine-Based Platform for Anti-Cancer Drug Delivery","volume":"7","author":"Ambekar","year":"2019","journal-title":"Biomater. 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