{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T03:59:42Z","timestamp":1783742382167,"version":"3.55.0"},"reference-count":81,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T00:00:00Z","timestamp":1700697600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004482","name":"College of Graduate Studies and the Research Sector of Kuwait University","doi-asserted-by":"publisher","award":["YP02\/21"],"award-info":[{"award-number":["YP02\/21"]}],"id":[{"id":"10.13039\/501100004482","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004482","name":"College of Graduate Studies and the Research Sector of Kuwait University","doi-asserted-by":"publisher","award":["SRUL02\/13"],"award-info":[{"award-number":["SRUL02\/13"]}],"id":[{"id":"10.13039\/501100004482","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004482","name":"College of Graduate Studies and the Research Sector of Kuwait University","doi-asserted-by":"publisher","award":["GM01\/13"],"award-info":[{"award-number":["GM01\/13"]}],"id":[{"id":"10.13039\/501100004482","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Research Core Facility, HSC, KU","award":["YP02\/21"],"award-info":[{"award-number":["YP02\/21"]}]},{"name":"Research Core Facility, HSC, KU","award":["SRUL02\/13"],"award-info":[{"award-number":["SRUL02\/13"]}]},{"name":"Research Core Facility, HSC, KU","award":["GM01\/13"],"award-info":[{"award-number":["GM01\/13"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Diabetic neuropathy is an important long-term complication of diabetes. This study explored the hypothesis that hydrogen sulfide (H2S) ameliorates neuropathic pain by controlling antiapoptotic and pro-apoptotic processes. The effects of a slow-releasing H2S donor, GYY4137, on the expression of antiapoptotic and pro-apoptotic genes and proteins, such as B-cell lymphoma 2 (Bcl2) and Bcl-2-like protein 4 (Bax), as well as caspases, cyclooxygenase (COX)-1 and COX-2, monocytes\/macrophages, and endothelial cells, in the spinal cord of male Sprague-Dawley rats with streptozotocin-induced peripheral diabetic neuropathy, were investigated using reverse transcription-PCR, western blot and immunohistochemistry. The antihypoalgesic activities of GYY4137 on diabetic rats were evaluated using the tail flick test. Treatment of diabetic rats with GYY4137 attenuated thermal hypoalgesia and prevented both the diabetes-induced increase in Bax mRNA expression (p = 0.0032) and the diabetes-induced decrease in Bcl2 mRNA expression (p = 0.028). The GYY4137-treated diabetic group had increased COX-1 (p = 0.015), decreased COX-2 (p = 0.002), reduced caspase-7 and caspase-9 protein expression (p &lt; 0.05), and lower numbers of endothelial and monocyte\/macrophage cells (p &lt; 0.05) compared to the non-treated diabetic group. In summary, the current study demonstrated the protective properties of H2S, which prevented the development of neuropathy related behavior, and suppressed apoptosis activation pathways and inflammation in the spinal cord. H2S-releasing drugs could be considered as possible treatment options of diabetic peripheral neuropathy.<\/jats:p>","DOI":"10.3390\/ijms242316650","type":"journal-article","created":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T06:06:23Z","timestamp":1700719583000},"page":"16650","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Neuroprotective Effects of a Hydrogen Sulfide Donor in Streptozotocin-Induced Diabetic Rats"],"prefix":"10.3390","volume":"24","author":[{"given":"Abdulaziz M. F.","family":"Shayea","sequence":"first","affiliation":[{"name":"Department of Occupational Therapy, College of Allied Health Science, Kuwait University, P.O. Box 24923, Safat 13110, Kuwait"},{"name":"Molecular Biology Program, College of Graduate Studies, Kuwait University, P.O. Box 24923, Safat 13110, Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waleed M.","family":"Renno","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Medicine, Kuwait University, P.O. Box 24923, Safat 13110, Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bedoor","family":"Qabazard","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, College of Pharmacy, Kuwait University, P.O. Box 24923, Safat 13110, Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3132-5935","authenticated-orcid":false,"given":"Willias","family":"Masocha","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, College of Pharmacy, Kuwait University, P.O. 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