{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T11:11:02Z","timestamp":1781089862900,"version":"3.54.1"},"reference-count":54,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T00:00:00Z","timestamp":1700697600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences (NCATS), National Institutes of Health (NIH)","doi-asserted-by":"publisher","award":["UL1TR000101"],"award-info":[{"award-number":["UL1TR000101"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences (NCATS), National Institutes of Health (NIH)","doi-asserted-by":"publisher","award":["MD007597-31-5959"],"award-info":[{"award-number":["MD007597-31-5959"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences (NCATS), National Institutes of Health (NIH)","doi-asserted-by":"publisher","award":["CA262617-01"],"award-info":[{"award-number":["CA262617-01"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Institutes of Health (NIH)","doi-asserted-by":"publisher","award":["UL1TR000101"],"award-info":[{"award-number":["UL1TR000101"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Institutes of Health (NIH)","doi-asserted-by":"publisher","award":["MD007597-31-5959"],"award-info":[{"award-number":["MD007597-31-5959"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Institutes of Health (NIH)","doi-asserted-by":"publisher","award":["CA262617-01"],"award-info":[{"award-number":["CA262617-01"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Metabolic-dysfunction-associated steatotic liver disease (MASLD) is becoming the most common chronic liver disease worldwide and is of concern among African Americans (AA) in the United States. This pilot study evaluated the differential gene expressions and identified the signature genes in the disease pathways of AA individuals with MASLD. Blood samples were obtained from MASLD patients (n = 23) and non-MASLD controls (n = 24) along with their sociodemographic and medical details. Whole-blood transcriptomic analysis was carried out using Affymetrix Clarion-S Assay. A validation study was performed utilizing TaqMan Arrays coupled with Ingenuity Pathway Analysis (IPA) to identify the major disease pathways. Out of 21,448 genes in total, 535 genes (2.5%) were significantly (p &lt; 0.05) and differentially expressed when we compared the cases and controls. A significant overlap in the predominant differentially expressed genes and pathways identified in previous studies using hepatic tissue was observed. Of note, TGFB1 and E2F1 genes were upregulated, and HMBS was downregulated significantly. Hepatic fibrosis signaling is the top canonical pathway, and its corresponding biofunction contributes to the development of hepatocellular carcinoma. The findings address the knowledge gaps regarding how signature genes and functional pathways can be detected in blood samples (\u2018liquid biopsy\u2019) in AA MASLD patients, demonstrating the potential of the blood samples as an alternative non-invasive source of material for future studies.<\/jats:p>","DOI":"10.3390\/ijms242316654","type":"journal-article","created":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T06:06:23Z","timestamp":1700719583000},"page":"16654","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Transcriptomics of MASLD Pathobiology in African American Patients in the Washington DC Area \u2020"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6542-5163","authenticated-orcid":false,"given":"Tanmoy","family":"Mondal","sequence":"first","affiliation":[{"name":"Department of Biology, Howard University, Washington, DC 20059, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coleman I.","family":"Smith","sequence":"additional","affiliation":[{"name":"MedStar-Georgetown Transplantation Institute, Georgetown University School of Medicine, Washington, DC 20007, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher A.","family":"Loffredo","sequence":"additional","affiliation":[{"name":"Department of Oncology, Georgetown University, Washington, DC 20007, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth","family":"Quartey","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, College of Medicine, Howard University, Washington, DC 20007, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gemeyel","family":"Moses","sequence":"additional","affiliation":[{"name":"Department of Biology, Howard University, Washington, DC 20059, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles D.","family":"Howell","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, College of Medicine, Howard University, Washington, DC 20007, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brent","family":"Korba","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Immunology, Georgetown University, Washington, DC 20007, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Kwabi-Addo","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, College of Medicine, Howard University, Washington, DC 20059, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gail","family":"Nunlee-Bland","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Child Health, College of Medicine, Howard University, Washington, DC 20059, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leanna","family":"R. Rucker","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, MedStar Georgetown University Hospital, Washington, DC 20007, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jheannelle","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Biology, Howard University, Washington, DC 20059, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5280-7418","authenticated-orcid":false,"given":"Somiranjan","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Department of Biology, Howard University, Washington, DC 20059, USA"},{"name":"Departments of Pediatrics and Child Health, College of Medicine, Howard University, Washington, DC 20059, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1111\/joim.13035","article-title":"Ethnic influence on nonalcoholic fatty liver disease prevalence and lack of disease awareness in the United States, 2011\u20132016","volume":"287","author":"Le","year":"2020","journal-title":"J. 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