{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T17:47:44Z","timestamp":1781200064168,"version":"3.54.1"},"reference-count":75,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T00:00:00Z","timestamp":1700784000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013362","name":"Swiss Cancer Research Foundation","doi-asserted-by":"publisher","award":["KFS-4281-08-2017"],"award-info":[{"award-number":["KFS-4281-08-2017"]}],"id":[{"id":"10.13039\/501100013362","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013362","name":"Swiss Cancer Research Foundation","doi-asserted-by":"publisher","award":["SNSF 31003A_176038\/1"],"award-info":[{"award-number":["SNSF 31003A_176038\/1"]}],"id":[{"id":"10.13039\/501100013362","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"publisher","award":["KFS-4281-08-2017"],"award-info":[{"award-number":["KFS-4281-08-2017"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"publisher","award":["SNSF 31003A_176038\/1"],"award-info":[{"award-number":["SNSF 31003A_176038\/1"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Intussusceptive pillars, regarded as a hallmark of intussusceptive angiogenesis, have been described in developing vasculature of many organs and organisms. The aim of this study was to resolve the question about pillar formation and their further maturation employing zebrafish caudal vein plexus (CVP). The CVP development was monitored by in vivo confocal microscopy in high spatio-temporal resolution using the transgenic zebrafish model Fli1a:eGPF\/\/Gata1:dsRed. We tracked back the formation of pillars (diameter \u2264 4 \u00b5m) and intercapillary meshes (diameter &gt; 4 \u00b5m) and analysed their morphology and behaviour. Transluminal pillars in the CVP arose via a combination of sprouting, lumen expansion, and\/or the creation of intraluminal folds, and those mechanisms were not associated directly with blood flow. The follow-up of pillars indicated that one-third of them disappeared between 28 and 48 h post fertilisation (hpf), and of the remaining ones, only 1\/17 changed their cross-section area by &gt;50%. The majority of the bigger meshes (39\/62) increased their cross-section area by &gt;50%. Plexus simplification and the establishment of hierarchy were dominated by the dynamics of intercapillary meshes, which formed mainly via sprouting angiogenesis. These meshes were observed to grow, reshape, and merge with each other. Our observations suggested an alternative view on intussusceptive angiogenesis in the CVP.<\/jats:p>","DOI":"10.3390\/ijms242316703","type":"journal-article","created":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T06:51:28Z","timestamp":1700808688000},"page":"16703","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Transluminal Pillars\u2014Their Origin and Role in the Remodelling of the Zebrafish Caudal Vein Plexus"],"prefix":"10.3390","volume":"24","author":[{"given":"Helena","family":"R\u00f6ss","sequence":"first","affiliation":[{"name":"Institute of Anatomy, University of Bern, 3012 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dea","family":"Aaldijk","sequence":"additional","affiliation":[{"name":"Institute of Anatomy, University of Bern, 3012 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mykhailo","family":"Vladymyrov","sequence":"additional","affiliation":[{"name":"Data Science Laboratory, University of Bern, 3012 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adolfo","family":"Odriozola","sequence":"additional","affiliation":[{"name":"Institute of Anatomy, University of Bern, 3012 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentin","family":"Djonov","sequence":"additional","affiliation":[{"name":"Institute of Anatomy, University of Bern, 3012 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0098-2997(02)00096-1","article-title":"Intussusceptive angiogenesis\u2013\u2013The alternative to capillary sprouting","volume":"23","author":"Burri","year":"2002","journal-title":"Mol. Aspects Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1006\/mvre.1997.2022","article-title":"Quantitative Study of Intussusceptive Capillary Growth in the Chorioallantoic Membrane (CAM) of the Chicken Embryo","volume":"54","author":"Schlatter","year":"1997","journal-title":"Microvasc. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.mvr.2014.06.009","article-title":"Stretch-induced intussuceptive and sprouting angiogenesis in the chick chorioallantoic membrane","volume":"95","author":"Belle","year":"2014","journal-title":"Microvasc. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1002\/dvdy.10119","article-title":"Optimality in the developing vascular system: Branching remodeling by means of intussusception as an efficient adaptation mechanism","volume":"224","author":"Djonov","year":"2002","journal-title":"Dev. Dyn."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/mvre.1998.2081","article-title":"TIE1 and TIE2 Receptor Tyrosine Kinases Inversely Regulate Embryonic Angiogenesis by the Mechanism of Intussusceptive Microvascular Growth","volume":"56","author":"Patan","year":"1998","journal-title":"Microvasc. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/S0940-9602(99)80127-7","article-title":"Architecture of blood vessels in human fetal gastric corpus: A corrosion casting study","volume":"181","author":"Gorczyca","year":"1999","journal-title":"Ann. Anat.-Anat. Anz."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1007\/s00429-006-0104-2","article-title":"Spatial growth and pattern formation in the small intestine microvascular bed from larval to adult Xenopus laevis: A scanning electron microscope study of microvascular corrosion casts","volume":"211","author":"Lametschwandtner","year":"2006","journal-title":"Anat. Embryol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"9840","DOI":"10.1038\/s41598-018-27791-6","article-title":"Synergistic interaction of sprouting and intussusceptive angiogenesis during zebrafish caudal vein plexus development","volume":"8","author":"Karthik","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"dev201030","DOI":"10.1242\/dev.201030","article-title":"Temporally and regionally distinct morphogenetic processes govern zebrafish caudal fin blood vessel network expansion","volume":"150","author":"Leonard","year":"2023","journal-title":"Development"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/S0092-8674(00)80108-7","article-title":"Patterns and Emerging Mechanisms of the Angiogenic Switch during Tumorigenesis","volume":"86","author":"Hanahan","year":"1996","journal-title":"Cell"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/S0074-7696(08)62106-3","article-title":"Angiogenesis: Models and Modulators","volume":"159","author":"Cockerill","year":"1995","journal-title":"Int. Rev. Cytol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1093\/cvr\/cvm102","article-title":"Endothelial sprouting and angiogenesis: Matrix metalloproteinases in the lead","volume":"78","author":"Koolwijk","year":"2008","journal-title":"Cardiovasc. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"6327","DOI":"10.7150\/jca.34171","article-title":"Molecular Mechanisms and Anticancer Therapeutic Strategies in Vasculogenic Mimicry","volume":"10","author":"Zhang","year":"2019","journal-title":"J. Cancer"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.pharmthera.2016.01.006","article-title":"Tumor cell vascular mimicry: Novel targeting opportunity in melanoma","volume":"159","author":"Hendrix","year":"2016","journal-title":"Pharmacol. Ther."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/s00441-003-0784-3","article-title":"Vascular remodeling by intussusceptive angiogenesis","volume":"314","author":"Djonov","year":"2003","journal-title":"Cell Tissue Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4166","DOI":"10.1111\/j.1582-4934.2009.00828.x","article-title":"Regression and persistence: Remodelling in a tissue engineered axial vascular assembly","volume":"13","author":"Polykandriotis","year":"2009","journal-title":"J. Cell. Mol. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/11428862_3","article-title":"A Computational Model of Micro-vascular Growth","volume":"3516","author":"Sunderam","year":"2005","journal-title":"Computational Science\u2013ICCS 2005"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.mvr.2009.08.004","article-title":"Computational flow dynamics in a geometric model of intussusceptive angiogenesis","volume":"78","author":"Filipovic","year":"2009","journal-title":"Microvasc. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1007\/s10456-014-9428-3","article-title":"Intussusceptive angiogenesis: Expansion and remodeling of microvascular networks","volume":"17","author":"Mentzer","year":"2014","journal-title":"Angiogenesis"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1704","DOI":"10.1002\/dvdy.382","article-title":"Techniques used to assess intussusceptive angiogenesis: A systematic review","volume":"250","author":"Smeets","year":"2021","journal-title":"Dev. Dyn."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Ribatti, D., Solimando, A.G., and Pezzella, F. (2021). The Anti-VEGF(R) Drug Discovery Legacy: Improving Attrition Rates by Breaking the Vicious Cycle of Angiogenesis in Cancer. Cancers, 13.","DOI":"10.3390\/cancers13143433"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.ydbio.2012.09.018","article-title":"\u201cSprouting angiogenesis\u201d, a reappraisal","volume":"372","author":"Ribatti","year":"2012","journal-title":"Dev. Biol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1006\/mvre.1996.1989","article-title":"Implementation of Intussusceptive Microvascular Growth in the Chicken Chorioallantoic Membrane (CAM): 2. Pillar Formation by Capillary Fusion","volume":"53","author":"Patan","year":"1997","journal-title":"Microvasc. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1006\/mvre.1996.0009","article-title":"Implementation of Intussusceptive Microvascular Growth in the Chicken Chorioallantoic Membrane (CAM): 1. pillar formation by folding of the capillary wall","volume":"51","author":"Patan","year":"1996","journal-title":"Microvasc. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1016\/j.ajpath.2011.05.033","article-title":"A New Mechanism for Pillar Formation during Tumor-Induced Intussusceptive Angiogenesis: Inverse Sprouting","volume":"179","author":"Paku","year":"2011","journal-title":"Am. J. Pathol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1161\/01.RES.86.3.286","article-title":"Intussusceptive angiogenesis: Its role in embryonic vascular network formation","volume":"86","author":"Djonov","year":"2000","journal-title":"Circ. Res."},{"key":"ref_27","first-page":"3242","article-title":"Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope","volume":"139","author":"Kaufmann","year":"2012","journal-title":"Dev. Camb. Engl."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1038\/ncb2232","article-title":"Distinct signalling pathways regulate sprouting angiogenesis from the dorsal aorta and the axial vein","volume":"13","author":"Wiley","year":"2011","journal-title":"Nat. Cell Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1016\/j.immuni.2006.10.015","article-title":"Tracing hematopoietic precursor migration to successive hematopoietic organs during zebrafish development","volume":"25","author":"Murayama","year":"2006","journal-title":"Immunity"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1006\/dbio.2000.9995","article-title":"The vascular anatomy of the developing zebrafish: An atlas of embryonic and early larval development","volume":"230","author":"Isogai","year":"2001","journal-title":"Dev. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1159\/000338278","article-title":"Intussusceptive Angiogenesis: A Biologically Relevant Form of Angiogenesis","volume":"49","author":"Casteleyn","year":"2012","journal-title":"J. Vasc. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/s10456-012-9300-2","article-title":"Increased shear stress inhibits angiogenesis in veins and not arteries during vascular development","volume":"16","author":"Jahnsen","year":"2013","journal-title":"Angiogenesis"},{"key":"ref_33","first-page":"447","article-title":"VEGF-A Promotes Intussusceptive Angiogenesis in the Developing Chicken Chorioallantoic Membrane","volume":"17","author":"Baum","year":"2010","journal-title":"Microcirculation"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1007\/s10456-017-9543-z","article-title":"Effects of nintedanib on the microvascular architecture in a lung fibrosis model","volume":"20","author":"Ackermann","year":"2017","journal-title":"Angiogenesis"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1093\/jmicro\/dfr025","article-title":"The impact of VEGF and bFGF on vascular stereomorphology in the context of angiogenic neo-arborisation after vascular induction","volume":"60","author":"Polykandriotis","year":"2011","journal-title":"J. Electron Microsc."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"32137","DOI":"10.1038\/srep32137","article-title":"Factors regulating capillary remodeling in a reversible model of inflammatory corneal angiogenesis","volume":"6","author":"Mukwaya","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_37","first-page":"979","article-title":"Lack of Angiogenesis in Experimental Brain Metastases. J. Neuropathol","volume":"70","author":"Bugyik","year":"2011","journal-title":"Exp. Neurol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1016\/j.prp.2013.08.005","article-title":"Ultrastructural changes in blood vessels in epidermal growth factor treated experimental cutaneous wound model","volume":"209","year":"2013","journal-title":"Pathol.-Res. Pract."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1007\/s10456-015-9481-6","article-title":"Brain angioarchitecture and intussusceptive microvascular growth in a murine model of Krabbe disease","volume":"18","author":"Giacomini","year":"2015","journal-title":"Angiogenesis"},{"key":"ref_40","first-page":"613","article-title":"CD105\/Ki67 double immunostaining expression in liver metastasis from colon carcinoma","volume":"52","author":"Gaje","year":"2011","journal-title":"Romanian J. Morphol. Embryol. Rev. Roum. Morphol. Embryol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1007\/s10856-011-4258-y","article-title":"On model of angiogenesis and the mechanism in porous silk fibroin films","volume":"22","author":"Bai","year":"2011","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Dimova, I., Hlushchuk, R., Makanya, A., Styp-Rekowska, B., Ceau\u015fu, R., Flueckiger, S., Lang, S., Semela, D., Noble, F., and Chatterjee, S. (2013). Inhibition of Notch signaling induces extensive intussusceptive neo-angiogenesis by recruitment of mononuclear cells. Angiogenesis, 16.","DOI":"10.1007\/s10456-013-9366-5"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1007\/s10456-012-9294-9","article-title":"Anti-VEGFR2 and anti-IGF-1R-Adnectins inhibit Ewing\u2019s sarcoma A673-xenograft growth and normalize tumor vascular architecture","volume":"15","author":"Ackermann","year":"2012","journal-title":"Angiogenesis"},{"key":"ref_44","first-page":"14","article-title":"Study of Intussusceptive Angiogenesis in Inflammatory Regional Lymph Nodes by Scanning Electron Microscopy","volume":"73","author":"Verli","year":"2009","journal-title":"Microsc. Res. Tech."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/2040-2384-2-11","article-title":"Blood flow shapes intravascular pillar geometry in the chick chorioallantoic membrane","volume":"2","author":"Lee","year":"2010","journal-title":"J. Angiogenesis Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e62155","DOI":"10.7554\/eLife.62155","article-title":"Abortive intussusceptive angiogenesis causes multi-cavernous vascular malformations","volume":"10","author":"Li","year":"2021","journal-title":"eLife"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1038\/nm0603-653","article-title":"Angiogenesis in health and disease","volume":"9","author":"Carmeliet","year":"2003","journal-title":"Nat. Med."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1083\/jcb.200302047","article-title":"VEGF guides angiogenic sprouting utilizing endothelial tip cell filopodia","volume":"161","author":"Gerhardt","year":"2003","journal-title":"J. Cell Biol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"a006569","DOI":"10.1101\/cshperspect.a006569","article-title":"VEGF and Notch in Tip and Stalk Cell Selection","volume":"3","author":"Blanco","year":"2013","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"4031","DOI":"10.1242\/dev.097352","article-title":"Filopodia are dispensable for endothelial tip cell guidance","volume":"140","author":"Phng","year":"2013","journal-title":"Development"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s10456-012-9304-y","article-title":"VEGF over-expression in skeletal muscle induces angiogenesis by intussusception rather than sprouting","volume":"16","author":"Trani","year":"2013","journal-title":"Angiogenesis"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"eabg9509","DOI":"10.1126\/sciadv.abg9509","article-title":"Low-flow intussusception and metastable VEGFR2 signaling launch angiogenesis in ischemic muscle","volume":"7","author":"Arpino","year":"2021","journal-title":"Sci. Adv."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1899","DOI":"10.1016\/j.ajpath.2010.12.049","article-title":"The Effects of PTK787\/ZK222584, an Inhibitor of VEGFR and PDGFR\u03b2 Pathways, on Intussusceptive Angiogenesis and Glomerular Recovery from Thy1.1 Nephritis","volume":"178","author":"Wnuk","year":"2011","journal-title":"Am. J. Pathol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1212","DOI":"10.1038\/ki.2012.17","article-title":"Podocyte EphB4 signaling helps recovery from glomerular injury","volume":"81","author":"Wnuk","year":"2012","journal-title":"Kidney Int."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1002\/dvdy.20020","article-title":"VEGF coordinates interaction of pericytes and endothelial cells during vasculogenesis and experimental angiogenesis","volume":"230","author":"Hagedorn","year":"2004","journal-title":"Dev. Dyn."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"e45054","DOI":"10.15252\/embr.201745054","article-title":"EphrinB2\/EphB4 signaling regulates non-sprouting angiogenesis by VEGF","volume":"19","author":"Groppa","year":"2018","journal-title":"EMBO Rep."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1016\/S0092-8674(00)81436-1","article-title":"Molecular Distinction and Angiogenic Interaction between Embryonic Arteries and Veins Revealed by ephrin-B2 and Its Receptor Eph-B4","volume":"93","author":"Wang","year":"1998","journal-title":"Cell"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"4681","DOI":"10.1007\/s11033-020-05470-y","article-title":"The critical role of the interplays of EphrinB2\/EphB4 and VEGF in the induction of angiogenesis","volume":"47","author":"Du","year":"2020","journal-title":"Mol. Biol. Rep."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Chen, D., Van Der Ent, M.A., Lartey, N.L., and King, P.D. (2023). EPHB4-RASA1-Mediated Negative Regulation of Ras-MAPK Signaling in the Vasculature: Implications for the Treatment of EPHB4- and RASA1-Related Vascular Anomalies in Humans. Pharmaceuticals, 16.","DOI":"10.3390\/ph16020165"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.semcdb.2021.06.005","article-title":"Endothelial cell mechanics and blood flow forces in vascular morphogenesis","volume":"120","author":"Phng","year":"2021","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1006\/dbio.2002.0711","article-title":"In vivo imaging of embryonic vascular development using transgenic zebrafish","volume":"248","author":"Lawson","year":"2002","journal-title":"Dev. Biol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.1038\/ni1007","article-title":"Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants","volume":"4","author":"Traver","year":"2003","journal-title":"Nat. Immunol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1038\/ng875","article-title":"Cardiac troponin T is essential in sarcomere assembly and cardiac contractility","volume":"31","author":"Sehnert","year":"2002","journal-title":"Nat. Genet."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"3","DOI":"10.2174\/1566524018666180322153432","article-title":"Blood Flow Regulates Zebrafish Caudal Vein Plexus Angiogenesis by ERK5-klf2a-nos2b Signaling","volume":"18","author":"Xie","year":"2018","journal-title":"Curr. Mol. Med."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1002\/ar.1092160207","article-title":"Scanning electron microscope study of the developing microvasculature in the postnatal rat lung","volume":"216","author":"Caduff","year":"1986","journal-title":"Anat. Rec."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/s10456-011-9236-y","article-title":"Spatial dependence of alveolar angiogenesis in post-pneumonectomy lung growth","volume":"15","author":"Konerding","year":"2012","journal-title":"Angiogenesis"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1111\/jmi.12300","article-title":"Three-dimensional image analytical detection of intussusceptive pillars in murine lung","volume":"260","author":"Wagner","year":"2015","journal-title":"J. Microsc."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1002\/dvdy.20184","article-title":"Intussusceptive angiogenesis: Its emergence, its characteristics, and its significance","volume":"231","author":"Burri","year":"2004","journal-title":"Dev. Dyn."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.devcel.2014.11.024","article-title":"Cdc42 Mediates Bmp-Induced Sprouting Angiogenesis through Fmnl3-Driven Assembly of Endothelial Filopodia in Zebrafish","volume":"32","author":"Wakayama","year":"2015","journal-title":"Dev. Cell"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1161\/01.RES.0000191547.64391.e3","article-title":"Endothelial extracellular matrix: Biosynthesis, remodeling, and functions during vascular morphogenesis and neovessel stabilization","volume":"97","author":"Davis","year":"2005","journal-title":"Circ. Res."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1002\/ar.23599","article-title":"Piecemeal Mechanism Combining Sprouting and Intussusceptive Angiogenesis in Intravenous Papillary Formation Induced by PGE2 and Glycerol","volume":"300","author":"Madrid","year":"2017","journal-title":"Anat. Rec."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Greysson-Wong, J., Rode, R., Ryu, J.-R., Rinker, K.D., and Childs, S.J. (2021). Venous activation of MEK\/ERK drives development of arteriovenous malformation and blood flow anomalies with loss of Rasa1. Dev. Biol.","DOI":"10.1101\/2021.07.21.453236"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"2774","DOI":"10.1172\/JCI67084","article-title":"RASA1 functions in EPHB4 signaling pathway to suppress endothelial mTORC1 activity","volume":"124","author":"Kawasaki","year":"2014","journal-title":"J. Clin. Invest."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1177\/27.10.512319","article-title":"Lead asparate, an en bloc contrast stain particularly useful for ultrastructural enzymology","volume":"27","author":"Walton","year":"1979","journal-title":"J. Histochem. Cytochem."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.jim.2016.08.004","article-title":"Real-time tissue offset correction system for intravital multiphoton microscopy","volume":"438","author":"Vladymyrov","year":"2016","journal-title":"J. Immunol. 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