{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T16:09:52Z","timestamp":1790352592618,"version":"4.1.0"},"reference-count":46,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T00:00:00Z","timestamp":1701129600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005363","name":"University of Buenos Aires","doi-asserted-by":"publisher","award":["20020170100116BA"],"award-info":[{"award-number":["20020170100116BA"]}],"id":[{"id":"10.13039\/501100005363","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005363","name":"University of Buenos Aires","doi-asserted-by":"publisher","award":["PICT-2019-0051"],"award-info":[{"award-number":["PICT-2019-0051"]}],"id":[{"id":"10.13039\/501100005363","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"AGENCIA NACIONAL DE PROMOCI\u00d3N CIENT\u00cdFICA Y TECNOL\u00d3GICA (ANPCyT)","doi-asserted-by":"publisher","award":["20020170100116BA"],"award-info":[{"award-number":["20020170100116BA"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"AGENCIA NACIONAL DE PROMOCI\u00d3N CIENT\u00cdFICA Y TECNOL\u00d3GICA (ANPCyT)","doi-asserted-by":"publisher","award":["PICT-2019-0051"],"award-info":[{"award-number":["PICT-2019-0051"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The angiotensin II type 2 (AT2) receptor has a role in promoting insulin sensitivity. However, the mechanisms underlying the AT2 receptor-induced facilitation of insulin are still not completely understood. Therefore, we investigated whether acute in vivo administration of AT2 receptor agonist compound 21 (C21) could activate insulin signaling molecules in insulin-target tissues. We report that, in male C57BL\/6 mice, an acute (5 min, 0.25 mg\/kg; i.v.) injection of C21 induces the phosphorylation of Akt and ERK1\/2 at activating residues (Ser473 and Thr202\/Tyr204, respectively) in both epididymal white adipose tissue (WAT) and heart tissue. In WAT, the extent of phosphorylation (p) of Akt and ERK1\/2 induced by C21 was approximately 65% of the level detected after a bolus injection of a dose of insulin known to induce maximal activation of the insulin receptor (IR). In the heart, C21 stimulated p-Akt to a lesser extent than in WAT and stimulated p-ERK1\/2 to similar levels to those attained by insulin administration. C21 did not modify p-IR levels in either tissue. We conclude that in vivo injection of the AT2 receptor agonist C21 activates Akt and ERK1\/2 through a mechanism that does not involve the IR, indicating the participation of these enzymes in AT2R-mediated signaling.<\/jats:p>","DOI":"10.3390\/ijms242316839","type":"journal-article","created":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T06:45:35Z","timestamp":1701153935000},"page":"16839","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Acute In Vivo Administration of Compound 21 Stimulates Akt and ERK1\/2 Phosphorylation in Mouse Heart and Adipose Tissue"],"prefix":"10.3390","volume":"24","author":[{"given":"Diego T.","family":"Quiroga","sequence":"first","affiliation":[{"name":"Facultad de Farmacia y Bioqu\u00edmica, Departamento de Qu\u00edmica Biol\u00f3gica and IQUIFIB (UBA-CONICET), Universidad de Buenos Aires, Buenos Aires C1113AAD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge A.","family":"Narva\u00e9z Pardo","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia y Bioqu\u00edmica, Departamento de Qu\u00edmica Biol\u00f3gica and IQUIFIB (UBA-CONICET), Universidad de Buenos Aires, Buenos Aires C1113AAD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda G.","family":"Zubir\u00eda","sequence":"additional","affiliation":[{"name":"Laboratorio de Neuroendocrinolog\u00eda, Instituto Multidisciplinario de Biolog\u00eda Celular (IMBICE), CICPBA-CONICET-UNLP), La Plata B1906APO, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjam\u00edn","family":"Barrales","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia y Bioqu\u00edmica, Departamento de Qu\u00edmica Biol\u00f3gica and IQUIFIB (UBA-CONICET), Universidad de Buenos Aires, Buenos Aires C1113AAD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina C.","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia y Bioqu\u00edmica, Departamento de Qu\u00edmica Biol\u00f3gica and IQUIFIB (UBA-CONICET), Universidad de Buenos Aires, Buenos Aires C1113AAD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s","family":"Giovambattista","sequence":"additional","affiliation":[{"name":"Laboratorio de Neuroendocrinolog\u00eda, Instituto Multidisciplinario de Biolog\u00eda Celular (IMBICE), CICPBA-CONICET-UNLP), La Plata B1906APO, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando P.","family":"Dominici","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia y Bioqu\u00edmica, Departamento de Qu\u00edmica Biol\u00f3gica and IQUIFIB (UBA-CONICET), Universidad de Buenos Aires, Buenos Aires C1113AAD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2158","DOI":"10.1172\/JCI119752","article-title":"Angiotensin II inhibits insulin signaling in aortic smooth muscle cells at multiple levels. 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