{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:55:35Z","timestamp":1785891335244,"version":"3.56.0"},"reference-count":57,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Foundation for Research in Cystic Fibrosis","award":["FFC#10\/2010"],"award-info":[{"award-number":["FFC#10\/2010"]}]},{"name":"Italian Foundation for Research in Cystic Fibrosis","award":["PE 00000007"],"award-info":[{"award-number":["PE 00000007"]}]},{"name":"PNRR PROJECT INF ACT \u201cOne Health Basic and Translational Research Actions addressing Unmet Needs on Emerging Infectious Diseases\u201d","award":["FFC#10\/2010"],"award-info":[{"award-number":["FFC#10\/2010"]}]},{"name":"PNRR PROJECT INF ACT \u201cOne Health Basic and Translational Research Actions addressing Unmet Needs on Emerging Infectious Diseases\u201d","award":["PE 00000007"],"award-info":[{"award-number":["PE 00000007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Pseudomonas aeruginosa is one of the six antimicrobial-resistant pathogens known as \u201cESKAPE\u201d that represent a global threat to human health and are considered priority targets for the development of novel antimicrobials and alternative therapeutics. The virulence of P. aeruginosa is regulated by a four-chemicals communication system termed quorum sensing (QS), and one main class of QS signals is termed acylhomoserine lactones (acyl-HSLs), which includes 3-Oxo-dodecanoil homoserine lactone (3-Oxo-C12-HSL), which regulates the expression of genes implicated in virulence and biofilm formation. Lactonases, like Paraoxonase 2 (PON2) from humans and the phosphotriesterase-like lactonases (PLLs) from thermostable microorganisms, are able to hydrolyze acyl-HSLs. In this work, we explored in vitro and in an animal model the effect of some lactonases on the production of Pseudomonas virulence factors. This study presents a model of chronic infection in which bacteria were administered by feeding, and Drosophila adults were treated with enzymes and the antibiotic tobramycin, alone or in combination. In vitro, we observed significant effects of lactonases on biofilm formation as well as effects on bacterial motility and the expression of virulence factors. The treatment in vivo by feeding with the lactonase SacPox allowed us to significantly increase the biocidal effect of tobramycin in chronic infection.<\/jats:p>","DOI":"10.3390\/ijms242317028","type":"journal-article","created":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T03:58:02Z","timestamp":1701403082000},"page":"17028","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Thermostable Lactonases Inhibit Pseudomonas aeruginosa Biofilm: Effect In Vitro and in Drosophila melanogaster Model of Chronic Infection"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8018-9752","authenticated-orcid":false,"given":"Elena","family":"Porzio","sequence":"first","affiliation":[{"name":"Institute of Biochemistry and Cell Biology, National Research Council of Italy, Via P. Castellino 111, 80131 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davide","family":"Andrenacci","sequence":"additional","affiliation":[{"name":"CNR Institute of Molecular Genetics \u201cLuigi-Luca Cavalli-Sforza\u201d Unit of Bologna, 40136 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6191-3952","authenticated-orcid":false,"given":"Giuseppe","family":"Manco","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Cell Biology, National Research Council of Italy, Via P. Castellino 111, 80131 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.18869\/modares.iem.2.1.25","article-title":"Pseudomonas aeruginosa: A review of their pathogenesis and prevalence in clinical settings and the environment","volume":"2","author":"Streeter","year":"2016","journal-title":"Infect. Epidemiol. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1128\/am.28.6.987-991.1974","article-title":"Agricultural plants and soil as a reservoir for Pseudomonas aeruginosa","volume":"28","author":"Green","year":"1974","journal-title":"Appl. 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