{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:23:52Z","timestamp":1791915832515,"version":"4.3.4"},"reference-count":42,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T00:00:00Z","timestamp":1702512000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Agricultural Fund for Rural Development","award":["00020.DDD.6509.00006.2018.04"],"award-info":[{"award-number":["00020.DDD.6509.00006.2018.04"]}]},{"name":"Wroclaw University of Environmental and Life Sciences","award":["00020.DDD.6509.00006.2018.04"],"award-info":[{"award-number":["00020.DDD.6509.00006.2018.04"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Yolkin is a polypeptide complex isolated from hen egg yolk that exhibits immunomodulating properties. The aim of the present study was to determine whether in-ovo-delivered yolkin affects leukocyte populations and cytokine levels in broiler chickens. The experiment was carried out on eggs from Ross 308 broiler breeder birds. Yolkin was administered in ovo on the 18th day of incubation, once, at the following three doses: 1, 10, or 100 \u00b5g\/egg. The immunological parameters were assessed in 1-, 7-, 14-, 21-, 28-, 35-, and 42-day-old birds kept under farming conditions and routinely vaccinated. The leukocyte populations were determined in the thymus, spleen, and blood. The cytokine (IL-1\u03b2, IL-2, IL-6, and IL-10) levels were determined in the plasma of the broiler chickens. Each experimental group included eight birds. The most pronounced effect of yolkin was an increase in the population of T cells, both CD4+ and CD8+, mainly in the blood. This effect on the lymphocyte subsets may be valuable regarding chicken immune responses, mainly against T-dependent antigens, during infection or after vaccination.<\/jats:p>","DOI":"10.3390\/ijms242417494","type":"journal-article","created":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T03:16:33Z","timestamp":1702610193000},"page":"17494","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Yolkin, a Polypeptide Complex from Egg Yolk, Affects Cytokine Levels and Leukocyte Populations in Broiler Chicken Blood and Lymphoid Organs after In Ovo Administration"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7526-3230","authenticated-orcid":false,"given":"Marianna","family":"Szczypka","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Wroc\u0142aw University of Environmental and Life Sciences, Norwida 31, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Lis","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Wroc\u0142aw University of Environmental and Life Sciences, Norwida 31, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2861-6275","authenticated-orcid":false,"given":"Maciej","family":"Kuczkowski","sequence":"additional","affiliation":[{"name":"Department of Epizootiology and Clinic of Birds and Exotic Animals, Faculty of Veterinary Medicine, Wroc\u0142aw University of Environmental and Life Sciences, Pl. Grunwaldzki 45, 50-366 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7214-073X","authenticated-orcid":false,"given":"Kamila","family":"Bobrek","sequence":"additional","affiliation":[{"name":"Department of Epizootiology and Clinic of Birds and Exotic Animals, Faculty of Veterinary Medicine, Wroc\u0142aw University of Environmental and Life Sciences, Pl. Grunwaldzki 45, 50-366 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7449-1661","authenticated-orcid":false,"given":"Aleksandra","family":"Pawlak","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Wroc\u0142aw University of Environmental and Life Sciences, Norwida 31, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4781-5296","authenticated-orcid":false,"given":"Aleksandra","family":"Zambrowicz","sequence":"additional","affiliation":[{"name":"Department of Functional Food Products Development, Faculty of Biotechnology and Food Science, Wroc\u0142aw University of Environmental and Life Sciences, Che\u0142mo\u0144skiego 37, 51-640 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Gawe\u0142","sequence":"additional","affiliation":[{"name":"Department of Epizootiology and Clinic of Birds and Exotic Animals, Faculty of Veterinary Medicine, Wroc\u0142aw University of Environmental and Life Sciences, Pl. Grunwaldzki 45, 50-366 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9511-3504","authenticated-orcid":false,"given":"Bo\u017cena","family":"Obmi\u0144ska-Mrukowicz","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Wroc\u0142aw University of Environmental and Life Sciences, Norwida 31, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,14]]},"reference":[{"key":"ref_1","unstructured":"(2023, December 05). World Health Organization website. Available online: https:\/\/www.who.int\/news-room\/questions-and-answers\/item\/one-health."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Nardulli, P., Ballini, A., Zamparella, M., and De Vito, D. (2023). 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