{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T22:11:39Z","timestamp":1790201499798,"version":"4.1.0"},"reference-count":42,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,12,21]],"date-time":"2023-12-21T00:00:00Z","timestamp":1703116800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007686","name":"Instituto Nacional de Perinatologia","doi-asserted-by":"publisher","award":["212250-3210-21201-02-16"],"award-info":[{"award-number":["212250-3210-21201-02-16"]}],"id":[{"id":"10.13039\/501100007686","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Endothelial dysfunction (ED) in preeclampsia (PE) results from the convergence of oxidative stress, inflammation, and alterations in extracellular matrix components, affecting vascular tone and permeability. The molecular network leading to ED includes IL-8 and MMP-2. In vitro, IL-8 regulates the concentration and activity of MMP-2 in the trophoblast; this interaction has not been studied in endothelial cells during PE. We isolated human umbilical vein endothelial cells (HUVECs) from women with healthy pregnancies (NP, n = 15) and PE (n = 15). We quantified the intracellular concentration of nitric oxide and reactive oxygen species with colorimetric assays, IL-8 with ELISA, and MMP-2 with zymography and using an ELISA-type system. An IL-8 inhibition assay was used to study the influence of this cytokine on MMP-2 concentration and activity. HUVECs from women with PE showed significantly higher oxidative stress than NP. IL-8 and MMP-2 were found to be significantly elevated in PE HUVECs compared to NP. Inhibition of IL-8 in HUVECs from women with PE significantly decreased the concentration of MMP-2. We demonstrate that IL-8 is involved in the mechanisms of MMP-2 expression in HUVECs from women with PE. Our findings provide new insights into the molecular mechanisms regulating the ED distinctive of PE.<\/jats:p>","DOI":"10.3390\/ijms25010122","type":"journal-article","created":{"date-parts":[[2023,12,21]],"date-time":"2023-12-21T03:36:17Z","timestamp":1703129777000},"page":"122","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Regulation of MMP-2 by IL-8 in Vascular Endothelial Cells: Probable Mechanism for Endothelial Dysfunction in Women with Preeclampsia"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7876-6098","authenticated-orcid":false,"given":"Arturo","family":"Flores-Pliego","sequence":"first","affiliation":[{"name":"Department of Immunobiochemistry, Instituto Nacional de Perinatolog\u00eda, Mexico City 11000, Mexico"},{"name":"Postgraduate in Experimental Biology, Division of Biological and Health Sciences, Universidad Aut\u00f3noma Metropolitana-Iztapalapa, Mexico City 09310, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9087-8017","authenticated-orcid":false,"given":"Aurora","family":"Espejel-Nu\u00f1ez","sequence":"additional","affiliation":[{"name":"Department of Immunobiochemistry, Instituto Nacional de Perinatolog\u00eda, Mexico City 11000, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7701-9621","authenticated-orcid":false,"given":"Hector","family":"Borboa-Olivares","sequence":"additional","affiliation":[{"name":"Community Interventions Research Branch, Instituto Nacional de Perinatolog\u00eda, Mexico City 11000, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5672-5228","authenticated-orcid":false,"given":"Sandra Berenice","family":"Parra-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Department of Immunobiochemistry, Instituto Nacional de Perinatolog\u00eda, Mexico City 11000, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5057-2793","authenticated-orcid":false,"given":"Araceli","family":"Montoya-Estrada","sequence":"additional","affiliation":[{"name":"Coordination of Gynecological and Perinatal Endocrinology, Instituto Nacional de Perinatolog\u00eda, Mexico City 11000, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3792-9094","authenticated-orcid":false,"given":"Humberto","family":"Gonz\u00e1lez-M\u00e1rquez","sequence":"additional","affiliation":[{"name":"Health Science Department, Universidad Aut\u00f3noma Metropolitana-Iztapalapa, Mexico City 09310, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8668-3943","authenticated-orcid":false,"given":"Ram\u00f3n","family":"Gonz\u00e1lez-Camarena","sequence":"additional","affiliation":[{"name":"Health Science Department, Universidad Aut\u00f3noma Metropolitana-Iztapalapa, Mexico City 09310, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guadalupe","family":"Estrada-Gutierrez","sequence":"additional","affiliation":[{"name":"Research Division, Instituto Nacional de Perinatolog\u00eda, Mexico City 11000, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.emc.2019.01.008","article-title":"Hypertensive Disorders of Pregnancy","volume":"37","author":"Wilkerson","year":"2019","journal-title":"Emerg. 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