{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T20:54:20Z","timestamp":1773089660150,"version":"3.50.1"},"reference-count":57,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,12,25]],"date-time":"2023-12-25T00:00:00Z","timestamp":1703462400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI17\/02019"],"award-info":[{"award-number":["PI17\/02019"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI20\/00094"],"award-info":[{"award-number":["PI20\/00094"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"name":"\u201cReto Everest\u201d from the Asociaci\u00f3n Espa\u00f1ola Contra el C\u00e1ncer (AECC)","award":["PI17\/02019"],"award-info":[{"award-number":["PI17\/02019"]}]},{"name":"\u201cReto Everest\u201d from the Asociaci\u00f3n Espa\u00f1ola Contra el C\u00e1ncer (AECC)","award":["PI20\/00094"],"award-info":[{"award-number":["PI20\/00094"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Current diagnostic algorithms are insufficient for the optimal clinical and therapeutic management of cutaneous spitzoid tumors, particularly atypical spitzoid tumors (AST). Therefore, it is crucial to identify new markers that allow for reliable and reproducible diagnostic assessment and can also be used as a predictive tool to anticipate the individual malignant potential of each patient, leading to tailored individual therapy. Using Reduced Representation Bisulfite Sequencing (RRBS), we studied genome\u2013wide methylation profiles of a series of Spitz nevi (SN), spitzoid melanoma (SM), and AST. We established a diagnostic algorithm based on the methylation status of seven cg sites located in TETK4P2 (Tektin 4 Pseudogene 2), MYO1D (Myosin ID), and PMF1-BGLAP (PMF1-BGLAP Readthrough), which allows the distinction between SN and SM but is also capable of subclassifying AST according to their similarity to the methylation levels of Spitz nevi or spitzoid melanoma. Thus, our epigenetic algorithm can predict the risk level of AST and predict its potential clinical outcomes.<\/jats:p>","DOI":"10.3390\/ijms25010318","type":"journal-article","created":{"date-parts":[[2023,12,25]],"date-time":"2023-12-25T23:00:12Z","timestamp":1703545212000},"page":"318","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Diagnostic Algorithm to Subclassify Atypical Spitzoid Tumors in Low and High Risk According to Their Methylation Status"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9832-3355","authenticated-orcid":false,"given":"Jose Francisco","family":"Gonz\u00e1lez-Mu\u00f1oz","sequence":"first","affiliation":[{"name":"Skin Cancer Research Group, Biomedical Research Institute INCLIVA, 46010 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9115-9249","authenticated-orcid":false,"given":"Beatriz","family":"S\u00e1nchez-Sendra","sequence":"additional","affiliation":[{"name":"Skin Cancer Research Group, Biomedical Research Institute INCLIVA, 46010 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9381-9976","authenticated-orcid":false,"given":"Carlos","family":"Monteagudo","sequence":"additional","affiliation":[{"name":"Skin Cancer Research Group, Biomedical Research Institute INCLIVA, 46010 Valencia, Spain"},{"name":"Department of Pathology, University of Valencia, 46010 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/s13643-017-0477-8","article-title":"The diagnosis and management of the Spitz nevus in the pediatric population: A systematic review and meta-analysis protocol","volume":"6","author":"Abboud","year":"2017","journal-title":"Syst. 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