{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:04:36Z","timestamp":1791407076918,"version":"4.3.3"},"reference-count":100,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T00:00:00Z","timestamp":1703635200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Research and Higher Education, France","award":["#AP-RM-22-012"],"award-info":[{"award-number":["#AP-RM-22-012"]}]},{"name":"Fondation de l\u2019Avenir","award":["#AP-RM-22-012"],"award-info":[{"award-number":["#AP-RM-22-012"]}]},{"name":"Algerian Ministry of Higher Education and Scientific Research","award":["#AP-RM-22-012"],"award-info":[{"award-number":["#AP-RM-22-012"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Fibrosis is a common feature of cardiovascular diseases and targets multiple organs, such as the heart and vessels. Endothelial to mesenchymal transition is a complex, vital process that occurs during embryonic formation and plays a crucial role in cardiac development. It is also a fundamental process implicated in cardiac fibrosis and repair, but also in other organs. Indeed, in numerous cardiovascular diseases, the endothelial-to-mesenchymal transition has been shown to be involved in the generation of fibroblasts that are able to produce extracellular matrix proteins such as type I collagen. This massive deposition results in tissue stiffening and organ dysfunction. To advance our understanding of this process for the development of new specific diagnostic and therapeutic strategies, it is essential to develop relevant cellular and animal models of this process. In this review, our aim was to gain an in-depth insight into existing in vitro and in vivo models of endothelial to mesenchymal transition in cardiovascular diseases with a focus on cardiac fibrosis. We discuss important parameters impacting endothelial to mesenchymal transition, and we give perspectives for the development of relevant models to decipher the underlying mechanisms and ultimately find new treatments specific to fibrosis happening in cardiovascular diseases.<\/jats:p>","DOI":"10.3390\/ijms25010382","type":"journal-article","created":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T10:23:54Z","timestamp":1703672634000},"page":"382","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Experimental Models to Study Endothelial to Mesenchymal Transition in Myocardial Fibrosis and Cardiovascular Diseases"],"prefix":"10.3390","volume":"25","author":[{"given":"Mohammed","family":"Mimouni","sequence":"first","affiliation":[{"name":"Biocommunication in Cardio-Metabolism (BC2M), University of Montpellier, 34000 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5912-5233","authenticated-orcid":false,"given":"Anne-Dominique","family":"Lajoix","sequence":"additional","affiliation":[{"name":"Biocommunication in Cardio-Metabolism (BC2M), University of Montpellier, 34000 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Desmetz","sequence":"additional","affiliation":[{"name":"Biocommunication in Cardio-Metabolism (BC2M), University of Montpellier, 34000 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"886553","DOI":"10.3389\/fcvm.2022.886553","article-title":"Cardiac Fibrosis in the Pressure Overloaded Left and Right Ventricle as a Therapeutic Target","volume":"9","author":"Schimmel","year":"2022","journal-title":"Front. Cardiovasc. Med."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Maruyama, K., and Imanaka-Yoshida, K. (2022). The Pathogenesis of Cardiac Fibrosis: A Review of Recent Progress. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23052617"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.1093\/cvr\/cvaa324","article-title":"Cardiac Fibrosis","volume":"117","author":"Frangogiannis","year":"2021","journal-title":"Cardiovasc. Res."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Kruszewska, J., Cudnoch-Jedrzejewska, A., and Czarzasta, K. (2022). Remodeling and Fibrosis of the Cardiac Muscle in the Course of Obesity-Pathogenesis and Involvement of the Extracellular Matrix. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23084195"},{"key":"ref_5","first-page":"e008372","article-title":"Endothelial-Mesenchymal Transition in Heart Failure with a Preserved Ejection Fraction: Insights into the Cardiorenal Syndrome","volume":"14","author":"Oh","year":"2021","journal-title":"Circ. Heart Fail."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.semcdb.2019.10.015","article-title":"Endothelium-Mediated Contributions to Fibrosis","volume":"101","author":"Sun","year":"2020","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"306","DOI":"10.2174\/18756557MTAwsMjA3w","article-title":"Endothelial to Mesenchymal Transition in the Cardiogenesis and Cardiovascular Diseases","volume":"16","author":"Anbara","year":"2020","journal-title":"Curr. Cardiol. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Peng, Q., Shan, D., Cui, K., Li, K., Zhu, B., Wu, H., Wang, B., Wong, S., Norton, V., and Dong, Y. (2022). The Role of Endothelial-to-Mesenchymal Transition in Cardiovascular Disease. Cells, 11.","DOI":"10.3390\/cells11111834"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.jacc.2018.09.089","article-title":"Endothelial to Mesenchymal Transition in Cardiovascular Disease: JACC State-of-the-Art Review","volume":"73","author":"Kovacic","year":"2019","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/j.ejcb.2018.07.005","article-title":"EndMT: A Promising and Controversial Field","volume":"97","author":"Hong","year":"2018","journal-title":"Eur. J. Cell Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1080\/17476348.2020.1795832","article-title":"Endothelial to Mesenchymal Transition (EndMT) and Vascular Remodeling in Pulmonary Hypertension and Idiopathic Pulmonary Fibrosis","volume":"14","author":"Gaikwad","year":"2020","journal-title":"Expert. Rev. Respir. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1089\/ars.2020.8169","article-title":"Endothelial-to-Mesenchymal Transition in Pulmonary Arterial Hypertension","volume":"34","author":"Gorelova","year":"2021","journal-title":"Antioxid. Redox Signal."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1093\/cvr\/cvx253","article-title":"Endothelial-Mesenchymal Transition in Atherosclerosis","volume":"114","author":"Souilhol","year":"2018","journal-title":"Cardiovasc. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2407","DOI":"10.1161\/CIRCULATIONAHA.110.938217","article-title":"Endothelial Cell-Derived Endothelin-1 Promotes Cardiac Fibrosis in Diabetic Hearts through Stimulation of Endothelial-to-Mesenchymal Transition","volume":"121","author":"Widyantoro","year":"2010","journal-title":"Circulation"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.ijcard.2011.06.052","article-title":"Effects of Angiotensin II Receptor Blocker on Myocardial Endothelial-to-Mesenchymal Transition in Diabetic Rats","volume":"162","author":"Tang","year":"2013","journal-title":"Int. J. Cardiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2734","DOI":"10.1016\/j.jacc.2014.02.572","article-title":"Endothelial NADPH Oxidase-2 Promotes Interstitial Cardiac Fibrosis and Diastolic Dysfunction through Proinflammatory Effects and Endothelial-Mesenchymal Transition","volume":"63","author":"Murdoch","year":"2014","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1050540","DOI":"10.3389\/fendo.2023.1050540","article-title":"Endothelial-to-Mesenchymal Transition: An Underappreciated Mediator of Diabetic Complications","volume":"14","author":"Wang","year":"2023","journal-title":"Front. Endocrinol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.ijcard.2014.06.062","article-title":"Increased Concentration of Circulating Angiogenesis and Nitric Oxide Inhibitors Induces Endothelial to Mesenchymal Transition and Myocardial Fibrosis in Patients with Chronic Kidney Disease","volume":"176","author":"Charytan","year":"2014","journal-title":"Int. J. Cardiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4007","DOI":"10.1016\/j.cell.2023.07.036","article-title":"TGF-\u03b2 Signaling in Health and Disease","volume":"186","author":"Sheppard","year":"2023","journal-title":"Cell"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Goumans, M.-J., and Ten Dijke, P. (2018). TGF-\u03b2 Signaling in Control of Cardiovascular Function. Cold Spring Harb. Perspect. Biol., 10.","DOI":"10.1101\/cshperspect.a022210"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"196","DOI":"10.3109\/08977194.2011.595714","article-title":"TGF-\u03b2 Signaling in Fibrosis","volume":"29","author":"Biernacka","year":"2011","journal-title":"Growth Factors"},{"key":"ref_22","first-page":"431","article-title":"Ligand-Specific Function of Transforming Growth Factor Beta in Epithelial-Mesenchymal Transition in Heart Development","volume":"238","author":"Azhar","year":"2009","journal-title":"Dev. Dyn. Off. Publ. Am. Assoc. Anat."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"8418","DOI":"10.1002\/jcp.26801","article-title":"Isoform-Specific Effects of Transforming Growth Factor \u03b2 on Endothelial-to-Mesenchymal Transition","volume":"233","author":"Sabbineni","year":"2018","journal-title":"J. Cell. Physiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1042\/BJ20101500","article-title":"Transforming Growth Factor-\u03922 Promotes Snail-Mediated Endothelial-Mesenchymal Transition through Convergence of Smad-Dependent and Smad-Independent Signalling","volume":"437","author":"Medici","year":"2011","journal-title":"Biochem. J."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Ursoli Ferreira, F., Eduardo Botelho Souza, L., Hassibe Thom\u00e9, C., Tomazini Pinto, M., Origassa, C., Salustiano, S., Marcel Fa\u00e7a, V., Olsen C\u00e2mara, N., Kashima, S., and Tadeu Covas, D. (2019). Endothelial Cells Tissue-Specific Origins Affects Their Responsiveness to TGF-\u03922 during Endothelial-to-Mesenchymal Transition. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20030458"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Pinto, M.T., Covas, D.T., Kashima, S., and Rodrigues, C.O. (2016). Endothelial Mesenchymal Transition: Comparative Analysis of Different Induction Methods. Biol. Proced. Online, 18.","DOI":"10.1186\/s12575-016-0040-3"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1111\/1440-1681.13802","article-title":"Diosmetin-7-O-\u03b2-D-Glucopyranoside Suppresses Endothelial-Mesenchymal Transformation through Endoplasmic Reticulum Stress in Cardiac Fibrosis","volume":"50","author":"Wang","year":"2023","journal-title":"Clin. Exp. Pharmacol. Physiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"12799","DOI":"10.1111\/jcmm.15871","article-title":"MFGE8 Is Down-Regulated in Cardiac Fibrosis and Attenuates Endothelial-Mesenchymal Transition through Smad2\/3-Snail Signalling Pathway","volume":"24","author":"Wang","year":"2020","journal-title":"J. Cell. Mol. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e142548","DOI":"10.1172\/JCI142548","article-title":"miR-181b Targets Semaphorin 3A to Mediate TGF-\u03b2-Induced Endothelial-Mesenchymal Transition Related to Atrial Fibrillation","volume":"132","author":"Lai","year":"2022","journal-title":"J. Clin. Investig."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3905","DOI":"10.7150\/thno.38640","article-title":"Serelaxin Alleviates Cardiac Fibrosis through Inhibiting Endothelial-to-Mesenchymal Transition via RXFP1","volume":"10","author":"Wilhelmi","year":"2020","journal-title":"Theranostics"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.peptides.2018.09.001","article-title":"Ghrelin Attenuates Myocardial Fibrosis after Acute Myocardial Infarction via Inhibiting Endothelial-to Mesenchymal Transition in Rat Model","volume":"111","author":"Chen","year":"2019","journal-title":"Peptides"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Rinastiti, P., Ikeda, K., Rahardini, E.P., Miyagawa, K., Tamada, N., Kuribayashi, Y., Hirata, K.-I., and Emoto, N. (2020). Loss of Family with Sequence Similarity 13, Member A Exacerbates Pulmonary Hypertension through Accelerating Endothelial-to-Mesenchymal Transition. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0226049"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.yjmcc.2018.12.002","article-title":"Inhibition of BRD4 Attenuates Transverse Aortic Constriction- and TGF-\u03b2-Induced Endothelial-Mesenchymal Transition and Cardiac Fibrosis","volume":"127","author":"Song","year":"2019","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.biopha.2017.07.104","article-title":"MiR-200a Modulates TGF-\u03921-Induced Endothelial-to-Mesenchymal Shift via Suppression of GRB2 in HAECs","volume":"95","author":"Zhang","year":"2017","journal-title":"Biomed. Pharmacother."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1002\/path.5193","article-title":"Inflammation Induces Endothelial-to-Mesenchymal Transition and Promotes Vascular Calcification through Downregulation of BMPR2","volume":"247","author":"Geerts","year":"2019","journal-title":"J. Pathol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2357","DOI":"10.1161\/ATVBAHA.121.313788","article-title":"Endothelial-Mesenchymal Transition in Cardiovascular Disease","volume":"41","author":"Alvandi","year":"2021","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_37","first-page":"4065","article-title":"Endothelial Cells from Different Anatomical Origin Have Distinct Responses during SNAIL\/TGF-\u03922-Mediated Endothelial-Mesenchymal Transition","volume":"10","author":"Pinto","year":"2018","journal-title":"Am. J. Transl. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1161\/CIRCRESAHA.120.318124","article-title":"MIR503HG Loss Promotes Endothelial-to-Mesenchymal Transition in Vascular Disease","volume":"128","author":"Monteiro","year":"2021","journal-title":"Circ. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2385","DOI":"10.1111\/cas.14455","article-title":"TNF-\u03b1 Enhances TGF-\u03b2-Induced Endothelial-to-Mesenchymal Transition via TGF-\u03b2 Signal Augmentation","volume":"111","author":"Yoshimatsu","year":"2020","journal-title":"Cancer Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.imbio.2012.05.026","article-title":"IL-1\u03b2 and TGF\u03b22 Synergistically Induce Endothelial to Mesenchymal Transition in an NF\u03baB-Dependent Manner","volume":"218","author":"Maleszewska","year":"2013","journal-title":"Immunobiology"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Kim, S., Lee, H., Moon, H., Kim, R., Kim, M., Jeong, S., Kim, H., Kim, S.H., Hwang, S.S., and Lee, M.Y. (2023). Epigallocatechin-3-Gallate Attenuates Myocardial Dysfunction via Inhibition of Endothelial-to-Mesenchymal Transition. Antioxidants, 12.","DOI":"10.3390\/antiox12051059"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1002\/path.5574","article-title":"miRNA-200c-3p Promotes Endothelial to Mesenchymal Transition and Neointimal Hyperplasia in Artery Bypass Grafts","volume":"253","author":"Chen","year":"2021","journal-title":"J. Pathol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1996","DOI":"10.1016\/j.ymthe.2018.05.002","article-title":"MicroRNA-148b Targets the TGF-\u03b2 Pathway to Regulate Angiogenesis and Endothelial-to-Mesenchymal Transition during Skin Wound Healing","volume":"26","author":"Miscianinov","year":"2018","journal-title":"Mol. Ther. J. Am. Soc. Gene Ther."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2660","DOI":"10.1016\/j.ajpath.2011.07.042","article-title":"Inflammation-Induced Endothelial-to-Mesenchymal Transition: A Novel Mechanism of Intestinal Fibrosis","volume":"179","author":"Rieder","year":"2011","journal-title":"Am. J. Pathol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2553","DOI":"10.18632\/aging.202290","article-title":"TLR2 Regulates Angiotensin II-Induced Vascular Remodeling and EndMT through NF-\u03baB Signaling","volume":"13","author":"Lin","year":"2020","journal-title":"Aging"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Jordan, N.P., Tingle, S.J., Shuttleworth, V.G., Cooke, K., Redgrave, R.E., Singh, E., Glover, E.K., Ahmad Tajuddin, H.B., Kirby, J.A., and Arthur, H.M. (2021). MiR-126-3p Is Dynamically Regulated in Endothelial-to-Mesenchymal Transition during Fibrosis. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22168629"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Zhang, Z.-Y., Zhai, C., Yang, X.-Y., Li, H.-B., Wu, L.-L., and Li, L. (2022). Knockdown of CD146 Promotes Endothelial-to-Mesenchymal Transition via Wnt\/\u03b2-Catenin Pathway. PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0273542"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"912660","DOI":"10.3389\/fphar.2022.912660","article-title":"An In Vitro Platform to Study Reversible Endothelial-to-Mesenchymal Transition","volume":"13","author":"Krishnamoorthi","year":"2022","journal-title":"Front. Pharmacol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1038\/s41419-020-02744-y","article-title":"Dual Roles of Chromatin Remodeling Protein BRG1 in Angiotensin II-Induced Endothelial-Mesenchymal Transition","volume":"11","author":"Li","year":"2020","journal-title":"Cell Death Dis."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1007\/s13577-021-00653-6","article-title":"The miR-214-3p\/c-Ski Axis Modulates Endothelial-Mesenchymal Transition in Human Coronary Artery Endothelial Cells in Vitro and in Mice Model in Vivo","volume":"35","author":"Wang","year":"2022","journal-title":"Hum. Cell"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2176","DOI":"10.1093\/hmg\/ddt614","article-title":"Hypoxia-Induced Epigenetic Modifications Are Associated with Cardiac Tissue Fibrosis and the Development of a Myofibroblast-like Phenotype","volume":"23","author":"Watson","year":"2014","journal-title":"Hum. Mol. Genet."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"16653","DOI":"10.1074\/jbc.M115.636944","article-title":"Snail Is a Direct Target of Hypoxia-Inducible Factor 1\u03b1 (HIF1\u03b1) in Hypoxia-Induced Endothelial to Mesenchymal Transition of Human Coronary Endothelial Cells","volume":"290","author":"Xu","year":"2015","journal-title":"J. Biol. Chem."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Sniegon, I., Prie\u00df, M., Heger, J., Schulz, R., and Euler, G. (2017). Endothelial Mesenchymal Transition in Hypoxic Microvascular Endothelial Cells and Paracrine Induction of Cardiomyocyte Apoptosis Are Mediated via TGF\u03b21\/SMAD Signaling. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18112290"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"eadc9465","DOI":"10.1126\/sciadv.adc9465","article-title":"Lactate Promotes Endothelial-to-Mesenchymal Transition via Snail1 Lactylation after Myocardial Infarction","volume":"9","author":"Fan","year":"2023","journal-title":"Sci. Adv."},{"key":"ref_55","first-page":"2278","article-title":"Endothelial-to-Mesenchymal Transition Shapes the Atherosclerotic Plaque and Modulates Macrophage Function","volume":"33","author":"Helmke","year":"2019","journal-title":"FASEB J. Off. Publ. Fed. Am. Soc. Exp. Biol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"e006680","DOI":"10.1161\/JAHA.117.006680","article-title":"Prolyl 4-Hydroxylase Domain Protein 3 Overexpression Improved Obstructive Sleep Apnea-Induced Cardiac Perivascular Fibrosis Partially by Suppressing Endothelial-to-Mesenchymal Transition","volume":"6","author":"Zhang","year":"2017","journal-title":"J. Am. Heart Assoc."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"4077","DOI":"10.1002\/jcp.26195","article-title":"Bone Morphogenetic Protein-7 Inhibits Endothelial-Mesenchymal Transition in Pulmonary Artery Endothelial Cell under Hypoxia","volume":"233","author":"Zhang","year":"2018","journal-title":"J. Cell. Physiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1016\/j.cellsig.2016.06.022","article-title":"The Role of PDGF-B\/TGF-\u03921\/Neprilysin Network in Regulating Endothelial-to-Mesenchymal Transition in Pulmonary Artery Remodeling","volume":"28","author":"Song","year":"2016","journal-title":"Cell. Signal."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"e141467","DOI":"10.1172\/JCI141467","article-title":"Endothelial FGF Signaling Is Protective in Hypoxia-Induced Pulmonary Hypertension","volume":"131","author":"Woo","year":"2021","journal-title":"J. Clin. Investig."},{"key":"ref_60","first-page":"270","article-title":"Hypoxia Induces Endothelial-mesenchymal Transition in Pulmonary Vascular Remodeling","volume":"42","author":"Zhang","year":"2018","journal-title":"Int. J. Mol. Med."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.lfs.2018.05.055","article-title":"The Role of miR-328 in High Glucose-Induced Endothelial-to-Mesenchymal Transition in Human Umbilical Vein Endothelial Cells","volume":"207","author":"Chen","year":"2018","journal-title":"Life Sci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"31053","DOI":"10.18632\/oncotarget.8842","article-title":"Inhibition of Myocyte-Specific Enhancer Factor 2A Improved Diabetic Cardiac Fibrosis Partially by Regulating Endothelial-to-Mesenchymal Transition","volume":"7","author":"Chen","year":"2016","journal-title":"Oncotarget"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.yexmp.2017.03.007","article-title":"High Glucose Induced Endothelial to Mesenchymal Transition in Human Umbilical Vein Endothelial Cell","volume":"102","author":"Yu","year":"2017","journal-title":"Exp. Mol. Pathol."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Tsai, T.-H., Lee, C.-H., Cheng, C.-I., Fang, Y.-N., Chung, S.-Y., Chen, S.-M., Lin, C.-J., Wu, C.-J., Hang, C.-L., and Chen, W.-Y. (2019). Liraglutide Inhibits Endothelial-to-Mesenchymal Transition and Attenuates Neointima Formation after Endovascular Injury in Streptozotocin-Induced Diabetic Mice. Cells, 8.","DOI":"10.3390\/cells8060589"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1111\/jcmm.14800","article-title":"Inhibition of miR-21 Alleviated Cardiac Perivascular Fibrosis via Repressing EndMT in T1DM","volume":"24","author":"Li","year":"2020","journal-title":"J. Cell. Mol. Med."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"7642","DOI":"10.1111\/jcmm.16601","article-title":"Dapagliflozin Alleviates Cardiac Fibrosis through Suppressing EndMT and Fibroblast Activation via AMPK\u03b1\/TGF-\u03b2\/Smad Signalling in Type 2 Diabetic Rats","volume":"25","author":"Tian","year":"2021","journal-title":"J. Cell. Mol. Med."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1186\/s10020-021-00339-7","article-title":"Ets1 Associates with KMT5A to Participate in High Glucose-Mediated EndMT via Upregulation of PFN2 Expression in Diabetic Nephropathy","volume":"27","author":"Lu","year":"2021","journal-title":"Mol. Med."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"709123","DOI":"10.3389\/fphys.2021.709123","article-title":"MicroRNA-195-5p Downregulation Inhibits Endothelial Mesenchymal Transition and Myocardial Fibrosis in Diabetic Cardiomyopathy by Targeting Smad7 and Inhibiting Transforming Growth Factor Beta 1-Smads-Snail Pathway","volume":"12","author":"Ding","year":"2021","journal-title":"Front. Physiol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1111\/1440-1681.13759","article-title":"Bach1 Modulates AKT3 Transcription to Participate in Hyperglycaemia-Mediated EndMT in Vascular Endothelial Cells","volume":"50","author":"Meng","year":"2023","journal-title":"Clin. Exp. Pharmacol. Physiol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/s13098-022-00966-y","article-title":"miR-132-3p and KLF7 as Novel Regulators of Aortic Stiffening-Associated EndMT in Type 2 Diabetes Mellitus","volume":"15","author":"Hulshoff","year":"2023","journal-title":"Diabetol. Metab. Syndr."},{"key":"ref_71","first-page":"1208","article-title":"MicroRNA-142-3p Inhibits High-Glucose-Induced Endothelial-to-Mesenchymal Transition through Targeting TGF-\u03921\/Smad Pathway in Primary Human Aortic Endothelial Cells","volume":"11","author":"Zhu","year":"2018","journal-title":"Int. J. Clin. Exp. Pathol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.bbrc.2017.07.101","article-title":"MiR-18a-5p Inhibits Endothelial-Mesenchymal Transition and Cardiac Fibrosis through the Notch2 Pathway","volume":"491","author":"Geng","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"629542","DOI":"10.3389\/fphys.2020.629542","article-title":"Calcium-Sensing Receptor Participates in High Glucose-Induced EndMT in Primary Human Aortic Endothelial Cells","volume":"11","author":"Yuan","year":"2020","journal-title":"Front. Physiol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"4801","DOI":"10.2147\/DMSO.S287287","article-title":"Sirt6-Mediated Endothelial-to-Mesenchymal Transition Contributes Toward Diabetic Cardiomyopathy via the Notch1 Signaling Pathway","volume":"13","author":"Zhang","year":"2020","journal-title":"Diabetes Metab. Syndr. Obes. Targets Ther."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"4207","DOI":"10.7150\/thno.48530","article-title":"A Novel Role of Kallikrein-Related Peptidase 8 in the Pathogenesis of Diabetic Cardiac Fibrosis","volume":"11","author":"Du","year":"2021","journal-title":"Theranostics"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"20304","DOI":"10.1038\/srep20304","article-title":"ROCK1 Induces Endothelial-to-Mesenchymal Transition in Glomeruli to Aggravate Albuminuria in Diabetic Nephropathy","volume":"6","author":"Peng","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1152\/physiolgenomics.00082.2016","article-title":"Serum Response Factor Induces Endothelial-Mesenchymal Transition in Glomerular Endothelial Cells to Aggravate Proteinuria in Diabetic Nephropathy","volume":"48","author":"Zhao","year":"2016","journal-title":"Physiol. Genom."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.3389\/fphar.2018.01213","article-title":"Losartan Alleviates Renal Fibrosis and Inhibits Endothelial-to-Mesenchymal Transition (EMT) Under High-Fat Diet-Induced Hyperglycemia","volume":"9","author":"Yao","year":"2018","journal-title":"Front. Pharmacol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1016\/j.jdiacomp.2018.06.011","article-title":"The Implication of Numb-Induced Notch Signaling in Endothelial-Mesenchymal Transition of Diabetic Nephropathy","volume":"32","author":"Liu","year":"2018","journal-title":"J. Diabetes Complicat."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1038\/s41420-022-00858-0","article-title":"Heparanase Promotes Endothelial-to-Mesenchymal Transition in Diabetic Glomerular Endothelial Cells through Mediating ERK Signaling","volume":"8","author":"Chang","year":"2022","journal-title":"Cell Death Discov."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"473","DOI":"10.3389\/fphar.2017.00473","article-title":"Lovastatin Alleviates Endothelial-to-Mesenchymal Transition in Glomeruli via Suppression of Oxidative Stress and TGF-\u03921 Signaling","volume":"8","author":"Ma","year":"2017","journal-title":"Front. Pharmacol."},{"key":"ref_82","first-page":"154700","article-title":"Rutin Alleviates EndMT by Restoring Autophagy through Inhibiting HDAC1 via PI3K\/AKT\/mTOR Pathway in Diabetic Kidney Disease","volume":"112","author":"Dong","year":"2023","journal-title":"Phytomed. Int. J. Phytother. Phytopharm."},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Guan, G., Xie, J., Dai, Y., and Han, H. (2022). TFPI2 Suppresses the Interaction of TGF-\u03922 Pathway Regulators to Promote Endothelial-Mesenchymal Transition in Diabetic Nephropathy. J. Biol. Chem., 298.","DOI":"10.1016\/j.jbc.2022.101725"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"13882","DOI":"10.1080\/21655979.2022.2086351","article-title":"Sprouty-Related Proteins with EVH1 Domain (SPRED2) Prevents High-Glucose Induced Endothelial-Mesenchymal Transition and Endothelial Injury by Suppressing MAPK Activation","volume":"13","author":"Liu","year":"2022","journal-title":"Bioengineered"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"110498","DOI":"10.1016\/j.ygeno.2022.110498","article-title":"m6A Transferase METTL3 Regulates Endothelial-Mesenchymal Transition in Diabetic Retinopathy via lncRNA SNHG7\/KHSRP\/MKL1 Axis","volume":"114","author":"Cao","year":"2022","journal-title":"Genomics"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"119232","DOI":"10.1016\/j.lfs.2021.119232","article-title":"MSC-Derived Exosomal lncRNA SNHG7 Suppresses Endothelial-Mesenchymal Transition and Tube Formation in Diabetic Retinopathy via miR-34a-5p\/XBP1 Axis","volume":"272","author":"Cao","year":"2021","journal-title":"Life Sci."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1139\/cjpp-2021-0265","article-title":"Downregulation of Acylglycerol Kinase Suppresses High-Glucose-Induced Endothelial-Mesenchymal Transition in Human Retinal Microvascular Endothelial Cells through Regulating the LPAR1\/TGF-\u03b2\/Notch Signaling Pathway","volume":"100","author":"Wang","year":"2022","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"ref_88","first-page":"3108","article-title":"miR-29a\/b Cluster Suppresses High Glucose-Induced Endothelial-Mesenchymal Transition in Human Retinal Microvascular Endothelial Cells by Targeting Notch2","volume":"17","author":"Zhang","year":"2019","journal-title":"Exp. Ther. Med."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"714573","DOI":"10.3389\/fcvm.2021.714573","article-title":"High Glucose Induced Changes in Human VEC Phenotype in a 3D Hydrogel Derived From Cell-Free Native Aortic Root","volume":"8","author":"Cecoltan","year":"2021","journal-title":"Front. Cardiovasc. Med."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1038\/s41569-018-0023-y","article-title":"Reassessing Endothelial-to-Mesenchymal Transition in Cardiovascular Diseases","volume":"15","author":"Li","year":"2018","journal-title":"Nat. Rev. Cardiol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1038\/nm888","article-title":"BMP-7 Counteracts TGF-Beta1-Induced Epithelial-to-Mesenchymal Transition and Reverses Chronic Renal Injury","volume":"9","author":"Zeisberg","year":"2003","journal-title":"Nat. Med."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1038\/nm1613","article-title":"Endothelial-to-Mesenchymal Transition Contributes to Cardiac Fibrosis","volume":"13","author":"Zeisberg","year":"2007","journal-title":"Nat. Med."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"H1363","DOI":"10.1152\/ajpheart.00395.2013","article-title":"Lack of Specificity of Fibroblast-Specific Protein 1 in Cardiac Remodeling and Fibrosis","volume":"305","author":"Kong","year":"2013","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"1488","DOI":"10.1038\/nm.4437","article-title":"Enhancing the Precision of Genetic Lineage Tracing Using Dual Recombinases","volume":"23","author":"He","year":"2017","journal-title":"Nat. Med."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"2921","DOI":"10.1172\/JCI74783","article-title":"Resident Fibroblast Lineages Mediate Pressure Overload-Induced Cardiac Fibrosis","volume":"124","author":"Banerjee","year":"2014","journal-title":"J. Clin. Investig."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"12260","DOI":"10.1038\/ncomms12260","article-title":"Genetic Lineage Tracing Defines Myofibroblast Origin and Function in the Injured Heart","volume":"7","author":"Kanisicak","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"227ra34","DOI":"10.1126\/scitranslmed.3006927","article-title":"TGF-\u03b2 Signaling Mediates Endothelial-to-Mesenchymal Transition (EndMT) during Vein Graft Remodeling","volume":"6","author":"Cooley","year":"2014","journal-title":"Sci. Transl. Med."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"11853","DOI":"10.1038\/ncomms11853","article-title":"Endothelial to Mesenchymal Transition Is Common in Atherosclerotic Lesions and Is Associated with Plaque Instability","volume":"7","author":"Evrard","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"2004","DOI":"10.1161\/CIRCULATIONAHA.121.055417","article-title":"Seamless Genetic Recording of Transiently Activated Mesenchymal Gene Expression in Endothelial Cells During Cardiac Fibrosis","volume":"144","author":"Zhang","year":"2021","journal-title":"Circulation"},{"key":"ref_100","doi-asserted-by":"crossref","unstructured":"Kurose, H. (2021). Cardiac Fibrosis and Fibroblasts. 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