{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T23:08:08Z","timestamp":1793833688171,"version":"4.6.1"},"reference-count":40,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T00:00:00Z","timestamp":1704672000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation of Korea (NRF)","award":["RS-2023-00208347"],"award-info":[{"award-number":["RS-2023-00208347"]}]},{"name":"National Research Foundation of Korea (NRF)","award":["HU20C0026"],"award-info":[{"award-number":["HU20C0026"]}]},{"DOI":"10.13039\/501100003725","name":"Korea government (MSIT)","doi-asserted-by":"publisher","award":["RS-2023-00208347"],"award-info":[{"award-number":["RS-2023-00208347"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"Korea government (MSIT)","doi-asserted-by":"publisher","award":["HU20C0026"],"award-info":[{"award-number":["HU20C0026"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Kore Health Industry Development Institute (KHIDI)","award":["RS-2023-00208347"],"award-info":[{"award-number":["RS-2023-00208347"]}]},{"name":"Kore Health Industry Development Institute (KHIDI)","award":["HU20C0026"],"award-info":[{"award-number":["HU20C0026"]}]},{"DOI":"10.13039\/501100003710","name":"Ministry of Health &amp; Welfare and Ministry of science and ICT","doi-asserted-by":"publisher","award":["RS-2023-00208347"],"award-info":[{"award-number":["RS-2023-00208347"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003710","name":"Ministry of Health &amp; Welfare and Ministry of science and ICT","doi-asserted-by":"publisher","award":["HU20C0026"],"award-info":[{"award-number":["HU20C0026"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The aging global population is placing an increasing burden on healthcare systems, and the social impact of Alzheimer\u2019s disease (AD) is on the rise. However, the availability of safe and effective treatments for AD remains limited. Adoptive Treg therapy has been explored for treating neurodegenerative diseases, including AD. To facilitate the clinical application of Treg therapy, we developed a Treg preparation protocol and highlighted the therapeutic effects of Tregs in 5xFAD mice. CD4+CD25+ Tregs, isolated after A\u03b2 stimulation and expanded using a G-rex plate with a gas-permeable membrane, were adoptively transferred into 5xFAD mice. Behavioral analysis was conducted using Y-maze and passive avoidance tests. Additionally, we measured levels of A\u03b2, phosphorylated tau (pTAU), and nitric oxide synthase 2 (NOS2) in the hippocampus. Real-time RT-PCR was employed to assess the mRNA levels of pro- and anti-inflammatory markers. Our findings indicate that A\u03b2-specific Tregs not only improved cognitive function but also reduced A\u03b2 and pTAU accumulation in the hippocampus of 5xFAD mice. They also inhibited microglial neuroinflammation. These effects were observed at doses as low as 1.5 \u00d7 103 cells\/head. Collectively, our results demonstrate that A\u03b2-specific Tregs can mitigate AD pathology in 5xFAD mice.<\/jats:p>","DOI":"10.3390\/ijms25020783","type":"journal-article","created":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T07:59:20Z","timestamp":1704700760000},"page":"783","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Therapeutic Effects of A\u03b2-Specific Regulatory T Cells in Alzheimer\u2019s Disease: A Study in 5xFAD Mice"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1072-2918","authenticated-orcid":false,"given":"Seon-Young","family":"Park","sequence":"first","affiliation":[{"name":"Department of Science in Korean Medicine, College of Korean Medicine, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8460-8027","authenticated-orcid":false,"given":"Juwon","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Korean Medicine, College of Korean Medicine, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1742-593X","authenticated-orcid":false,"given":"Hyejin","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Science in Korean Medicine, College of Korean Medicine, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inhee","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Korean Medicine, College of Korean Medicine, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3012-8200","authenticated-orcid":false,"given":"Jae Yoon","family":"Kim","sequence":"additional","affiliation":[{"name":"Institute of Life Science & Biotechnology, VT Bio. Co., Ltd., 16 Samseong-ro 76-gil, Gangnam-gu, Seoul 06185, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0299-3582","authenticated-orcid":false,"given":"Hyunsu","family":"Bae","sequence":"additional","affiliation":[{"name":"Department of Science in Korean Medicine, College of Korean Medicine, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea"},{"name":"Department of Korean Medicine, College of Korean Medicine, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,8]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2017). 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