{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T18:39:38Z","timestamp":1785523178635,"version":"3.56.0"},"reference-count":40,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T00:00:00Z","timestamp":1705017600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>HuR regulates cytoplasmic mRNA stability and translatability, with its expression correlating with adverse outcomes in various cancers. This study aimed to assess the prognostic value and pro-oncogenic properties of HuR and its post-translational isoforms methyl-HuR and phospho-HuR in endometrial adenocarcinoma. Examining 89 endometrioid adenocarcinomas, we analyzed the relationship between HuR nuclear or cytoplasmic immunostaining, tumor-cell proliferation, and patient survival. HuR cytoplasmic expression was significantly increased in grade 3 vs. grade 1 adenocarcinomas (p &lt; 0.001), correlating with worse overall survival (OS) (p = 0.02). Methyl-HuR cytoplasmic expression significantly decreased in grade 3 vs. grade 1 adenocarcinomas (p &lt; 0.001) and correlated with better OS (p = 0.002). Phospho-HuR nuclear expression significantly decreased in grade 3 vs. grade 1 adenocarcinomas (p &lt; 0.001) and non-significantly correlated with increased OS (p = 0.06). Cytoplasmic HuR expression strongly correlated with proliferation markers MCM6 (rho = 0.59 and p &lt; 0.001) and Ki67 (rho = 0.49 and p &lt; 0.001). Conversely, these latter inversely correlated with cytoplasmic methyl-HuR and nuclear phospho-HuR. Cytoplasmic HuR expression is a poor prognosis marker in endometrioid endometrial adenocarcinoma, while cytoplasmic methyl-HuR and nuclear phosphoHuR expressions are markers of better prognosis. This study highlights HuR as a promising potential therapeutic target, especially in treatment-resistant tumors, though further research is needed to understand the mechanisms regulating HuR subcellular localization and post-translational modifications.<\/jats:p>","DOI":"10.3390\/ijms25020954","type":"journal-article","created":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T07:47:16Z","timestamp":1705045636000},"page":"954","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Expressions of HuR, Methyl-HuR and Phospho-HuR in Endometrial Endometrioid Adenocarcinoma Are Associated with Clinical Features"],"prefix":"10.3390","volume":"25","author":[{"given":"Judica\u00ebl","family":"Hotton","sequence":"first","affiliation":[{"name":"Department of Gynecology and Obstetrics, CHRU de Nancy, Universit\u00e9 de Lorraine, 54000 Nancy, France"},{"name":"INSERM U1256 NGERE, Universit\u00e9 de Lorraine, 54500 Vand\u0153uvre-l\u00e8s-Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5585-9195","authenticated-orcid":false,"given":"Guillaume","family":"Gauchotte","sequence":"additional","affiliation":[{"name":"INSERM U1256 NGERE, Universit\u00e9 de Lorraine, 54500 Vand\u0153uvre-l\u00e8s-Nancy, France"},{"name":"Department of Biopathology CHRU of Nancy, Institut de Canc\u00e9rologie de Lorraine, BBB, CHRU de Nancy, Universit\u00e9 de Lorraine, 54511 Vand\u0153uvre-l\u00e8s-Nancy, France"},{"name":"Centre de Ressources Biologiques, BB-0033-00035, CHRU de Nancy, 54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7654-8150","authenticated-orcid":false,"given":"Romane","family":"Mougel","sequence":"additional","affiliation":[{"name":"INSERM U1256 NGERE, Universit\u00e9 de Lorraine, 54500 Vand\u0153uvre-l\u00e8s-Nancy, France"},{"name":"Department of Reproductive Medicine, CHRU de Nancy, Universit\u00e9 de Lorraine, 54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e9gane","family":"Migliorini","sequence":"additional","affiliation":[{"name":"INSERM U1256 NGERE, Universit\u00e9 de Lorraine, 54500 Vand\u0153uvre-l\u00e8s-Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Lacomme","sequence":"additional","affiliation":[{"name":"Centre de Ressources Biologiques, BB-0033-00035, CHRU de Nancy, 54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5881-6251","authenticated-orcid":false,"given":"Shyue-Fang","family":"Battaglia-Hsu","sequence":"additional","affiliation":[{"name":"INSERM U1256 NGERE, Universit\u00e9 de Lorraine, 54500 Vand\u0153uvre-l\u00e8s-Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5192-8923","authenticated-orcid":false,"given":"Mika\u00ebl","family":"Agopiantz","sequence":"additional","affiliation":[{"name":"INSERM U1256 NGERE, Universit\u00e9 de Lorraine, 54500 Vand\u0153uvre-l\u00e8s-Nancy, France"},{"name":"Department of Reproductive Medicine, CHRU de Nancy, Universit\u00e9 de Lorraine, 54000 Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.3322\/caac.21708","article-title":"Cancer statistics, 2022","volume":"72","author":"Siegel","year":"2022","journal-title":"CA Cancer J. 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