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While gliogenesis and glial differentiation occur during perinatal cerebellar development, the processes that occur during early postnatal development remain obscure. In this study, we conducted transcriptomic profiling of postnatal cerebellar astrocytes at postnatal days 1, 7, 14, and 28 (P1, P7, P14, and P28), identifying temporal-specific gene signatures at each specific time point. Comparing these profiles with region-specific astrocyte differentially expressed genes (DEGs) published for the cortex, hippocampus, and olfactory bulb revealed cerebellar-specific gene signature across these developmental timepoints. Moreover, we conducted a comparative analysis of cerebellar astrocyte gene signatures with gene lists from pediatric brain tumors of cerebellar origin, including ependymoma and medulloblastoma. Notably, genes downregulated at P14, such as Kif11 and HMGB2, exhibited significant enrichment across all pediatric brain tumor groups, suggesting the importance of astrocytic gene repression during cerebellar development to these tumor subtypes. Collectively, our studies describe gene expression patterns during cerebellar astrocyte development, with potential implications for pediatric tumors originating in the cerebellum.<\/jats:p>","DOI":"10.3390\/ijms25021021","type":"journal-article","created":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T04:03:26Z","timestamp":1705291406000},"page":"1021","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparative Transcriptomic Analysis of Cerebellar Astrocytes across Developmental Stages and Brain Regions"],"prefix":"10.3390","volume":"25","author":[{"given":"Wookbong","family":"Kwon","sequence":"first","affiliation":[{"name":"Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Cancer Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Department of Neurosurgery, Baylor College of Medicine, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong-Joo","family":"Choi","sequence":"additional","affiliation":[{"name":"Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Cancer Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Department of Neurosurgery, Baylor College of Medicine, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwanha","family":"Yu","sequence":"additional","affiliation":[{"name":"Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Cancer Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Department of Neurosurgery, Baylor College of Medicine, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Williamson","sequence":"additional","affiliation":[{"name":"Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Cancer Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Department of Neurosurgery, Baylor College of Medicine, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjana","family":"Murali","sequence":"additional","affiliation":[{"name":"Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Cancer Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Department of Neurosurgery, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Program in Cancer Cell Biology, Baylor College of Medicine, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeunjung","family":"Ko","sequence":"additional","affiliation":[{"name":"Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Cancer Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Department of Neurosurgery, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Program in Immunology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junsung","family":"Woo","sequence":"additional","affiliation":[{"name":"Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Cancer Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Department of Neurosurgery, Baylor College of Medicine, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Deneen","sequence":"additional","affiliation":[{"name":"Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Center for Cancer Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Department of Neurosurgery, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Program in Cancer Cell Biology, Baylor College of Medicine, Houston, TX 77030, USA"},{"name":"Program in Immunology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1038\/nrn.2016.159","article-title":"Functional Diversity of Astrocytes in Neural Circuit Regulation","volume":"18","author":"Haim","year":"2017","journal-title":"Nat. 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