{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T23:08:16Z","timestamp":1768086496115,"version":"3.49.0"},"reference-count":68,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T00:00:00Z","timestamp":1705276800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"National Science Centre","doi-asserted-by":"publisher","award":["2020\/38\/E\/NZ4\/00020"],"award-info":[{"award-number":["2020\/38\/E\/NZ4\/00020"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The discovery of mitochondria-derived peptides (MDPs) has provided a new perspective on mitochondrial function. MDPs encoded by mitochondrial DNA (mtDNA) can act as hormone-like peptides, influencing cell survival and proliferation. Among these peptides, humanin has been identified as a crucial factor for maintaining cell survival and preventing cell death under various conditions. Adrenocortical carcinoma (ACC) is a rare and aggressive malignancy that results from adrenal hormone dysfunction. This study aimed to investigate humanin expression in the adrenal tissue and serum of patients with ACC. For the first time, our study revealed significant reduction in the mRNA expression of humanin in patients with ACC compared to healthy controls. However, no significant changes were observed in the serum humanin levels. Interestingly, we identified a positive correlation between patient age and serum humanin levels and a negative correlation between tumor size and LDL levels. While the impaired expression of humanin in patients with ACC may be attributed to mitochondrial dysfunction, an alternative explanation could be related to diminished mitochondrial copy number. Further investigations are warranted to elucidate the intricate relationship among humanin, mitochondrial function, and ACC pathology.<\/jats:p>","DOI":"10.3390\/ijms25021038","type":"journal-article","created":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T04:03:26Z","timestamp":1705291406000},"page":"1038","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Impaired Expression of Humanin during Adrenocortical Carcinoma"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5506-2397","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Blatkiewicz","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0150-3665","authenticated-orcid":false,"given":"Marta","family":"Szyszka","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6043-5637","authenticated-orcid":false,"given":"Anna","family":"Olechnowicz","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland"},{"name":"Doctoral School, Poznan University of Medical Sciences, 60-812 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kacper","family":"Kami\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland"},{"name":"Doctoral School, Poznan University of Medical Sciences, 60-812 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7399-0303","authenticated-orcid":false,"given":"Karol","family":"Jopek","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanna","family":"Komarowska","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, 60-356 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0623-9496","authenticated-orcid":false,"given":"Marianna","family":"Tyczewska","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland"},{"name":"Department of Anatomy and Histology, University of Zielona G\u00f3ra, Licealna Street 9, 65-417 Zielona G\u00f3ra, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Klimont","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, 60-356 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomasz","family":"Wierzbicki","sequence":"additional","affiliation":[{"name":"Department of General, Endocrinological and Gastroenterological Surgery, Poznan University of Medical Sciences, 60-355 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marek","family":"Karczewski","sequence":"additional","affiliation":[{"name":"Department of General and Transplantation Surgery, Poznan University of Medical Sciences, 60-356 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6296-7220","authenticated-orcid":false,"given":"Marek","family":"Rucha\u0142a","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, 60-356 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2525-5777","authenticated-orcid":false,"given":"Marcin","family":"Rucinski","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"796","DOI":"10.18632\/aging.100943","article-title":"Naturally occurring mitochondrial-derived peptides are age-dependent regulators of apoptosis, insulin sensitivity, and inflammatory markers","volume":"8","author":"Cobb","year":"2016","journal-title":"Aging"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.cmet.2015.02.009","article-title":"The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance","volume":"21","author":"Lee","year":"2015","journal-title":"Cell Metab."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"6336","DOI":"10.1073\/pnas.101133498","article-title":"A rescue factor abolishing neuronal cell death by a wide spectrum of familial Alzheimer\u2019s disease genes and Abeta","volume":"98","author":"Hashimoto","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.1038\/s41380-022-01769-3","article-title":"Mitochondrial DNA variation in Alzheimer\u2019s disease reveals a unique microprotein called SHMOOSE","volume":"28","author":"Miller","year":"2023","journal-title":"Mol. Psychiatry"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Kienzle, L., Bettinazzi, S., Choquette, T., Brunet, M., Khorami, H.H., Jacques, J.F., Moreau, M., Roucou, X., Landry, C.R., and Angers, A. (2023). A small protein coded within the mitochondrial canonical gene nd4 regulates mitochondrial bioenergetics. BMC Biol., 21.","DOI":"10.1186\/s12915-023-01609-y"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/j.acvd.2020.03.020","article-title":"Role of humanin, a mitochondrial-derived peptide, in cardiovascular disorders","volume":"113","author":"Rochette","year":"2020","journal-title":"Arch. Cardiovasc. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"6613","DOI":"10.1113\/JP274472","article-title":"Mitochondrially derived peptides as novel regulators of metabolism","volume":"595","author":"Kim","year":"2017","journal-title":"J. Physiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1006\/bbrc.2001.4765","article-title":"Mechanisms of neuroprotection by a novel rescue factor humanin from Swedish mutant amyloid precursor protein","volume":"283","author":"Hashimoto","year":"2001","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.bbrc.2005.01.100","article-title":"Solution structure of humanin, a peptide against Alzheimer\u2019s disease-related neurotoxicity","volume":"329","author":"Benaki","year":"2005","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1038\/nature01627","article-title":"Humanin peptide suppresses apoptosis by interfering with Bax activation","volume":"423","author":"Guo","year":"2003","journal-title":"Nature"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e158449","DOI":"10.1172\/JCI158449","article-title":"Mitochondria-derived peptides in aging and healthspan","volume":"132","author":"Miller","year":"2022","journal-title":"J. Clin. Investig."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.cmet.2018.06.008","article-title":"The Mitochondrial-Encoded Peptide MOTS-c Translocates to the Nucleus to Regulate Nuclear Gene Expression in Response to Metabolic Stress","volume":"28","author":"Kim","year":"2018","journal-title":"Cell Metab."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"123","DOI":"10.3389\/fnagi.2019.00123","article-title":"Humanin, a Mitochondrial-Derived Peptide Released by Astrocytes, Prevents Synapse Loss in Hippocampal Neurons","volume":"11","author":"Zarate","year":"2019","journal-title":"Front. Aging Neurosci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"11185","DOI":"10.18632\/aging.103534","article-title":"The mitochondrial derived peptide humanin is a regulator of lifespan and healthspan","volume":"12","author":"Yen","year":"2020","journal-title":"Aging"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"14212","DOI":"10.1038\/s41598-018-32616-7","article-title":"Humanin Prevents Age-Related Cognitive Decline in Mice and is Associated with Improved Cognitive Age in Humans","volume":"8","author":"Yen","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1111\/acel.12243","article-title":"IGF-I regulates the age-dependent signaling peptide humanin","volume":"13","author":"Lee","year":"2014","journal-title":"Aging Cell"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1007\/s11357-020-00262-5","article-title":"Mitochondrial-derived peptides in aging and age-related diseases","volume":"43","author":"Kim","year":"2021","journal-title":"Geroscience"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"106","DOI":"10.3389\/fendo.2016.00106","article-title":"Adrenal Mitochondria and Steroidogenesis: From Individual Proteins to Functional Protein Assemblies","volume":"7","author":"Midzak","year":"2016","journal-title":"Front. Endocrinol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Vietor, C.L., Creemers, S.G., van Kemenade, F.J., van Ginhoven, T.M., Hofland, L.J., and Feelders, R.A. (2021). How to Differentiate Benign from Malignant Adrenocortical Tumors?. Cancers, 13.","DOI":"10.3390\/cancers13174383"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1530\/EJE-13-0519","article-title":"Adrenocortical carcinoma: Clinical outcomes and prognosis of 330 patients at a tertiary care center","volume":"169","author":"Jasim","year":"2013","journal-title":"Eur. J. Endocrinol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.1007\/s12094-020-02524-2","article-title":"Adrenal cancer: Relevance of different grading systems and subtypes","volume":"23","author":"Minner","year":"2021","journal-title":"Clin. Transl. Oncol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"209","DOI":"10.4149\/neo_2019_190105N17","article-title":"Clinical presentation, treatment modalities and outcome in patients with adrenocortical carcinoma: A single center experience","volume":"67","author":"Bronswijk","year":"2020","journal-title":"Neoplasma"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.ijcard.2017.07.014","article-title":"Pheochromocytoma as a reversible cause of cardiomyopathy: Analysis and review of the literature","volume":"249","author":"Zhang","year":"2017","journal-title":"Int. J. Cardiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1161\/01.HYP.29.5.1133","article-title":"Tumor recurrence and hypertension persistence after successful pheochromocytoma operation","volume":"29","author":"Plouin","year":"1997","journal-title":"Hypertension"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.urology.2017.12.007","article-title":"Metastatic Pheochromocytoma in an Asymptomatic 12-Year-Old with von Hippel-Lindau Disease","volume":"119","author":"Colvin","year":"2018","journal-title":"Urology"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1007\/s12022-009-9082-2","article-title":"Aldosterone-producing adrenal cortical adenoma with oncocytic change and cytoplasmic eosinophilic globular inclusions","volume":"20","author":"Mete","year":"2009","journal-title":"Endocr. Pathol."},{"key":"ref_27","first-page":"1278","article-title":"Frame shift by insertion of 2 basepairs in codon 394 of CYP11B1 causes congenital adrenal hyperplasia due to steroid 11 beta-hydroxylase deficiency","volume":"75","author":"Helmberg","year":"1992","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1080\/1354750X.2023.2246700","article-title":"Exploring the potential of humanin as a biomarker for early breast cancer detection: A study of serum levels and diagnostic performance","volume":"28","author":"Hekim","year":"2023","journal-title":"Biomarkers"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Pena Agudelo, J.A., Pidre, M.L., Garcia Fallit, M., Perez Kuper, M., Zuccato, C., Nicola Candia, A.J., Marchesini, A., Vera, M.B., De Simone, E., and Giampaoli, C. (2023). Mitochondrial Peptide Humanin Facilitates Chemoresistance in Glioblastoma Cells. Cancers, 15.","DOI":"10.3390\/cancers15164061"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/2008-2231-22-14","article-title":"Genome expression analysis by suppression subtractive hybridization identified overexpression of Humanin, a target gene in gastric cancer chemoresistance","volume":"22","author":"Sepehrizadeh","year":"2014","journal-title":"Daru"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.jnci.2017.04.001","article-title":"Breaking the ritual metabolic cycle in order to save acetyl CoA: A potential role for mitochondrial humanin in T2 bladder cancer aggressiveness","volume":"29","author":"Omar","year":"2017","journal-title":"J. Egypt. Natl. Canc Inst."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1085\/jgp.8.6.519","article-title":"The Metabolism of Tumors in the Body","volume":"8","author":"Warburg","year":"1927","journal-title":"J. Gen. Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1126\/science.123.3191.309","article-title":"On the origin of cancer cells","volume":"123","author":"Warburg","year":"1956","journal-title":"Science"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1101\/sqb.2005.70.035","article-title":"Mitochondria and cancer: Warburg addressed","volume":"70","author":"Wallace","year":"2005","journal-title":"Cold Spring Harb. Symp. Quant. Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1007\/s13167-020-00217-y","article-title":"Flavonoids against the Warburg phenotype-concepts of predictive, preventive and personalised medicine to cut the Gordian knot of cancer cell metabolism","volume":"11","author":"Samec","year":"2020","journal-title":"EPMA J."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Lee, Y.G., Park, D.H., and Chae, Y.C. (2022). Role of Mitochondrial Stress Response in Cancer Progression. Cells, 11.","DOI":"10.3390\/cells11050771"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.mad.2016.12.002","article-title":"Mitochondria and mitochondria-induced signalling molecules as longevity determinants","volume":"165","author":"Rose","year":"2017","journal-title":"Mech. Ageing Dev."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.mam.2011.10.007","article-title":"Hormesis, cellular stress response and vitagenes as critical determinants in aging and longevity","volume":"32","author":"Calabrese","year":"2011","journal-title":"Mol. Asp. Med."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1007\/s00281-020-00813-0","article-title":"Mitochondria, immunosenescence and inflammaging: A role for mitokines?","volume":"42","author":"Conte","year":"2020","journal-title":"Semin. Immunopathol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1093\/cvr\/cvq191","article-title":"Humanin is expressed in human vascular walls and has a cytoprotective effect against oxidized LDL-induced oxidative stress","volume":"88","author":"Bachar","year":"2010","journal-title":"Cardiovasc. Res."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Muzumdar, R.H., Huffman, D.M., Atzmon, G., Buettner, C., Cobb, L.J., Fishman, S., Budagov, T., Cui, L., Einstein, F.H., and Poduval, A. (2009). Humanin: A novel central regulator of peripheral insulin action. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0006334"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1093\/gerona\/gly153","article-title":"Human Aging and Longevity Are Characterized by High Levels of Mitokines","volume":"74","author":"Conte","year":"2019","journal-title":"J. Gerontol. A Biol. Sci. Med. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1111\/acel.12389","article-title":"The mitochondrial-derived peptide MOTS-c: A player in exceptional longevity?","volume":"14","author":"Fuku","year":"2015","journal-title":"Aging Cell"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1139\/cjpp-2014-0401","article-title":"The effect of sex on humanin levels in healthy adults and patients with uncomplicated type 1 diabetes mellitus","volume":"93","author":"Lytvyn","year":"2015","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.clinbiochem.2003.12.005","article-title":"Oxidative and antioxidative status in pregnant women with either gestational or type 1 diabetes","volume":"37","author":"Peuchant","year":"2004","journal-title":"Clin. Biochem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1007\/s00592-011-0264-2","article-title":"Oxidative stress and antioxidant status in patients with late-onset gestational diabetes mellitus","volume":"50","author":"Roca","year":"2013","journal-title":"Acta Diabetol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1177\/1535370216641787","article-title":"Role of mitochondrial dysfunction in cancer progression","volume":"241","author":"Hsu","year":"2016","journal-title":"Exp. Biol. Med."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"292","DOI":"10.3389\/fonc.2013.00292","article-title":"Mitochondrial dysfunction in cancer","volume":"3","author":"Boland","year":"2013","journal-title":"Front. Oncol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1196\/annals.1338.011","article-title":"Mitochondrial genome instability and mtDNA depletion in human cancers","volume":"1042","author":"Lee","year":"2005","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"674","DOI":"10.3390\/ijms10020674","article-title":"Mitochondrial DNA instability and metabolic shift in human cancers","volume":"10","author":"Lee","year":"2009","journal-title":"Int. J. Mol. Sci."},{"key":"ref_51","first-page":"899","article-title":"Correlation between increased copy number of mitochondrial DNA and clinicopathological stage in colorectal cancer","volume":"2","author":"Feng","year":"2011","journal-title":"Oncol. Lett."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.ygyno.2005.04.015","article-title":"The increase of mitochondrial DNA content in endometrial adenocarcinoma cells: A quantitative study using laser-captured microdissected tissues","volume":"98","author":"Wang","year":"2005","journal-title":"Gynecol. Oncol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/0304-3835(96)04276-0","article-title":"Mitochondrial DNA copy number changes in human gliomas","volume":"105","author":"Liang","year":"1996","journal-title":"Cancer Lett."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.mrfmmm.2003.12.011","article-title":"Somatic mutations in the D-loop and decrease in the copy number of mitochondrial DNA in human hepatocellular carcinoma","volume":"547","author":"Lee","year":"2004","journal-title":"Mutat. Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1002\/gcc.20326","article-title":"Mitochondrial DNA mutations and mitochondrial DNA depletion in breast cancer","volume":"45","author":"Tseng","year":"2006","journal-title":"Genes. Chromosomes Cancer"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Gottardo, M.F., Jaita, G., Magri, M.L., Zarate, S., Moreno Ayala, M., Ferraris, J., Eijo, G., Pisera, D., Candolfi, M., and Seilicovich, A. (2014). Antiapoptotic factor humanin is expressed in normal and tumoral pituitary cells and protects them from TNF-alpha-induced apoptosis. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0111548"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.cryobiol.2019.04.001","article-title":"Potent humanin analogue (HNG) protects human sperm from freeze-thaw-induced damage","volume":"88","author":"Yang","year":"2019","journal-title":"Cryobiology"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1002\/jcp.20672","article-title":"Anti-apoptotic factor humanin is expressed in the testis and prevents cell-death in leydig cells during the first wave of spermatogenesis","volume":"208","author":"Colon","year":"2006","journal-title":"J. Cell Physiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"3111","DOI":"10.1002\/jcb.26452","article-title":"S14G-humanin alleviates insulin resistance and increases autophagy in neurons of APP\/PS1 transgenic mouse","volume":"119","author":"Han","year":"2018","journal-title":"J. Cell Biochem."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"4890","DOI":"10.1096\/fj.13-231092","article-title":"Potent humanin analog increases glucose-stimulated insulin secretion through enhanced metabolism in the beta cell","volume":"27","author":"Kuliawat","year":"2013","journal-title":"FASEB J."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"2115","DOI":"10.1007\/s001250100021","article-title":"A choice of death--the signal-transduction of immune-mediated beta-cell apoptosis","volume":"44","author":"Eizirik","year":"2001","journal-title":"Diabetologia"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"213","DOI":"10.4239\/wjd.v13.i3.213","article-title":"Humanin and diabetes mellitus: A review of in vitro and in vivo studies","volume":"13","author":"Boutari","year":"2022","journal-title":"World J. Diabetes"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.bbrc.2018.02.071","article-title":"Humanin promotes mitochondrial biogenesis in pancreatic MIN6 beta-cells","volume":"497","author":"Qin","year":"2018","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.mce.2015.06.015","article-title":"Rat Humanin is encoded and translated in mitochondria and is localized to the mitochondrial compartment where it regulates ROS production","volume":"413","author":"Paharkova","year":"2015","journal-title":"Mol. Cell Endocrinol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.metabol.2009.08.001","article-title":"The neurosurvival factor Humanin inhibits beta-cell apoptosis via signal transducer and activator of transcription 3 activation and delays and ameliorates diabetes in nonobese diabetic mice","volume":"59","author":"Hoang","year":"2010","journal-title":"Metabolism"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"e13063","DOI":"10.14814\/phy2.13063","article-title":"Humanin skeletal muscle protein levels increase after resistance training in men with impaired glucose metabolism","volume":"4","author":"Gidlund","year":"2016","journal-title":"Physiol. Rep."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Blatkiewicz, M., Kaminski, K., Szyszka, M., Al-Shakarchi, Z., Olechnowicz, A., Stelcer, E., Komarowska, H., Tyczewska, M., Klimont, A., and Karczewski, M. (2023). The Enhanced Expression of ZWILCH Predicts Poor Survival of Adrenocortical Carcinoma Patients. Biomedicines, 11.","DOI":"10.3390\/biomedicines11041233"},{"key":"ref_68","unstructured":"(2023, November 16). The Open Lab Book. Available online: https:\/\/tholb.readthedocs.io\/en\/latest\/imaging\/measuring-cell-fluorescence-using-imagej.html."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/2\/1038\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:46:47Z","timestamp":1760104007000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/2\/1038"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,15]]},"references-count":68,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,1]]}},"alternative-id":["ijms25021038"],"URL":"https:\/\/doi.org\/10.3390\/ijms25021038","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,15]]}}}