{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:17:23Z","timestamp":1790399843536,"version":"4.1.0"},"reference-count":28,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T00:00:00Z","timestamp":1705536000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81872969"],"award-info":[{"award-number":["81872969"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2019227"],"award-info":[{"award-number":["2019227"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22SH14"],"award-info":[{"award-number":["22SH14"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The Science and the Youth Innovation Promotion Association of CAS","award":["81872969"],"award-info":[{"award-number":["81872969"]}]},{"name":"The Science and the Youth Innovation Promotion Association of CAS","award":["2019227"],"award-info":[{"award-number":["2019227"]}]},{"name":"The Science and the Youth Innovation Promotion Association of CAS","award":["22SH14"],"award-info":[{"award-number":["22SH14"]}]},{"name":"The Science and Technology Innovation Cooperation Project of Changchun Science and Technology Bureau and Chinese Academy of Sciences","award":["81872969"],"award-info":[{"award-number":["81872969"]}]},{"name":"The Science and Technology Innovation Cooperation Project of Changchun Science and Technology Bureau and Chinese Academy of Sciences","award":["2019227"],"award-info":[{"award-number":["2019227"]}]},{"name":"The Science and Technology Innovation Cooperation Project of Changchun Science and Technology Bureau and Chinese Academy of Sciences","award":["22SH14"],"award-info":[{"award-number":["22SH14"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Cell membrane chromatography (CMC) has been widely recognized as a highly efficient technique for in vitro screening of active compounds. Nevertheless, conventional CMC approaches suffer from a restricted repertoire of cell membrane proteins, making them susceptible to oversaturation. Moreover, the binding mechanism between silica gel and proteins primarily relies on intermolecular hydrogen bonding, which is inherently unstable and somewhat hampers the advancement of CMC. Consequently, this investigation aimed to establish a novel CMC column that could augment protein loading, enhance detection throughput, and bolster binding affinity through the introduction of covalent bonding with proteins. This study utilizes polydopamine (PDA)-coated silica gel, which is formed through the self-polymerization of dopamine (DA), as the carrier for the CMC column filler. The objective is to construct the HK-2\/SiO2-PDA\/CMC model to screen potential therapeutic drugs for gout. To compare the quantity and characteristics of Human Kidney-2 (HK-2) cell membrane proteins immobilized on SiO2-PDA and silica gel, the proteins were immobilized on both surfaces. The results indicate that SiO2-PDA has a notably greater affinity for membrane proteins compared to silica gel, resulting in a significant improvement in detection efficiency. Furthermore, a screening method utilizing HK-2\/SiO2-PDA\/CMC was utilized to identify seven potential anti-gout compounds derived from Plantago asiatica L. (PAL). The effectiveness of these compounds was further validated using an in vitro cell model of uric acid (UA) reabsorption. In conclusion, this study successfully developed and implemented a novel CMC filler, which has practical implications in the field.<\/jats:p>","DOI":"10.3390\/ijms25021153","type":"journal-article","created":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T03:50:56Z","timestamp":1705549856000},"page":"1153","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Establishment of Polydopamine-Modified HK-2 Cell Membrane Chromatography and Screening of Active Components from Plantago asiatica L."],"prefix":"10.3390","volume":"25","author":[{"given":"Hongxue","family":"Gao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Electroanalytical Chemistry & Jilin Provincial Key Laboratory of Chinese Medicine Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China"},{"name":"Institute of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei 230029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqiang","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Electroanalytical Chemistry & Jilin Provincial Key Laboratory of Chinese Medicine Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China"},{"name":"Institute of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei 230029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengrui","family":"Song","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Electroanalytical Chemistry & Jilin Provincial Key Laboratory of Chinese Medicine Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junpeng","family":"Xing","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Electroanalytical Chemistry & Jilin Provincial Key Laboratory of Chinese Medicine Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong","family":"Zheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Electroanalytical Chemistry & Jilin Provincial Key Laboratory of Chinese Medicine Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zong","family":"Hou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Electroanalytical Chemistry & Jilin Provincial Key Laboratory of Chinese Medicine Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Electroanalytical Chemistry & Jilin Provincial Key Laboratory of Chinese Medicine Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China"},{"name":"Institute of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei 230029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1007\/BF02885029","article-title":"Enzymatic activity and chromatographic characteristics of the cell membrane immobilized on silica surface","volume":"44","author":"He","year":"1999","journal-title":"Chin. Sci. Bull."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/BF02491836","article-title":"Coating and fusing cell membranes onto a silica surface and their chromatographic characteristics","volume":"54","author":"He","year":"2001","journal-title":"Chromatographia"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.jpba.2014.05.021","article-title":"Recent advances in cell membrane chromatography for traditional Chinese medicines analysis","volume":"101","author":"Hou","year":"2014","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"461916","DOI":"10.1016\/j.chroma.2021.461916","article-title":"Advances in cell membrane chromatography","volume":"1639","author":"Ma","year":"2021","journal-title":"J. Chromatogr. A"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"4521","DOI":"10.2174\/092986710794182926","article-title":"Pharmacodynamic studies of Chinese medicine at levels of whole animal, cell and molecular models","volume":"17","author":"Qv","year":"2010","journal-title":"Curr. Med. Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1007\/s10337-011-2085-4","article-title":"Prostate cell membrane Cchromatography-LC\/MS method for screening \u03b11A-adrenoceptor antagonists from Lotus Plumule","volume":"74","author":"Ren","year":"2011","journal-title":"Chromatographia"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"122492","DOI":"10.1016\/j.jchromb.2020.122492","article-title":"Comparative two-dimensional GPC3 overexpressing SK-Hep1 cell membrane chromatography\/C18\/time-of-flight mass spectrometry for screening selective GPC3 inhibitor components from Scutellariae Radix","volume":"1163","author":"Chen","year":"2021","journal-title":"J. Chromatogr. B"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1002\/jssc.202200200","article-title":"Enhanced stability designs of cell membrane chromatography for screening drug leads","volume":"45","author":"Fu","year":"2022","journal-title":"J. Sep. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1087404","DOI":"10.3389\/fphar.2022.1087404","article-title":"Screening of the active Ingredients in Huanglian Jiedu decoction through amide bond-Immobilized magnetic nanoparticle-assisted cell membrane chromatography","volume":"13","author":"Liao","year":"2022","journal-title":"Front. Pharmacol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2105","DOI":"10.1002\/jssc.201901203","article-title":"Preparation and application of polyvinyl alcohol-decorated cell membrane chromatography for screening anti-osteoporosis components from Liuwei Dihuang decoction-containing serum","volume":"43","author":"Wu","year":"2020","journal-title":"J. Sep. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1007\/s00604-018-2826-2","article-title":"Polydopamine-based molecularly imprinting polymers on magnetic nanoparticles for recognition and enrichment of ochratoxins prior to their determination by HPLC","volume":"185","author":"Hu","year":"2018","journal-title":"Microchim. Acta"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1002\/cssc.201601732","article-title":"One-pot anchoring of Pd nanoparticles on nitrogendoped carbon through dopamine self-polymerization and activity in the electrocatalytic methanol oxidation reaction","volume":"10","author":"Li","year":"2017","journal-title":"Chemsuschem"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1480","DOI":"10.1002\/jssc.201600026","article-title":"Polydopamine-coated magnetic molecularly imprinted polymer for the selective solid-phase extraction of cinnamic acid, ferulic acid and caffeic acid from radix scrophulariae sample","volume":"39","author":"Yin","year":"2016","journal-title":"J. Sep. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.electacta.2019.07.040","article-title":"Electrochemical chiral interface based on the Michael addition\/Schiff base reaction of polydopamine functionalized reduced graphene oxide","volume":"319","author":"Yang","year":"2019","journal-title":"Electrochim. Acta"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"649505","DOI":"10.3389\/fmed.2021.649505","article-title":"Urate Crystals; Beyond Joints","volume":"8","author":"Ahmad","year":"2021","journal-title":"Front. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1016\/j.semnephrol.2020.12.001","article-title":"Pathophysiology of Gout","volume":"40","author":"Narang","year":"2020","journal-title":"Semin. Nephrol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1136\/ard.2005.049023","article-title":"Gout, diuretics and the kidney","volume":"65","author":"Pascual","year":"2006","journal-title":"Ann. Rheum. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.1080\/14656566.2021.1918102","article-title":"Dotinurad: A novel selective urate reabsorption inhibitor for the treatment of hyperuricemia and gout","volume":"22","author":"Ishikawa","year":"2021","journal-title":"Expert Opin. Pharmacother."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"S4","DOI":"10.1186\/ar1909","article-title":"Recent developments in our understanding of the renal basis of hyperuricemia and the development of novel antihyperuricemic therapeutics","volume":"8","author":"Terkeltaub","year":"2006","journal-title":"Arthritis. Res. Ther."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Gao, H., Liu, Z., Song, F., Xing, J., Zheng, Z., and Liu, S. (2022). A Strategy for Identification and Structural Characterization of Compounds from Plantago asiatica L. by Liquid Chromatography-Mass Spectrometry Combined with Ion Mobility Spectrometry. Molecules, 27.","DOI":"10.3390\/molecules27134302"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.chroma.2014.07.071","article-title":"Quality improvements of cell membrane chromatographic column","volume":"1359","author":"Ding","year":"2014","journal-title":"J. Chromatogr. A"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"113825","DOI":"10.1016\/j.jpba.2020.113825","article-title":"A stop-flow comprehensive two-dimensional HK-2 and HK-2\/CIKI cell membrane chromatography comparative analysis system for screening the active ingredients from Pyrrosia calvata (Bak.) Ching against crystal-induced kidney injury","volume":"195","author":"Pan","year":"2021","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"155","DOI":"10.4103\/0976-500X.195897","article-title":"Lesinurad: A significant advancement or just another addition to existing therapies of gout?","volume":"7","author":"Gupta","year":"2016","journal-title":"J. Pharmacol. Pharmacother."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1763","DOI":"10.1021\/acsbiomaterials.2c00026","article-title":"Dexamethasone: Insights into pharmacologicalaspects, therapeutic mechanisms, and delivery systems","volume":"8","author":"Madamsetty","year":"2022","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4228","DOI":"10.1038\/s41467-018-06620-4","article-title":"Large-scale whole-exome sequencing association studies identify rare functional variants influencing serum urate levels","volume":"9","author":"Tin","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5224","DOI":"10.1093\/rheumatology\/keab327","article-title":"Substantial anti-gout effect conferred by common and rare dysfunctional variants of URAT1\/SLC22A12","volume":"60","author":"Toyoda","year":"2021","journal-title":"Rheumatology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1124\/jpet.115.231647","article-title":"Stronger uricosuric effects of the novel selective URAT1 inhibitor UR-1102 lowered plasma urate in tufted capuchin monkeys to a greater extent than benzbromarone","volume":"357","author":"Ahn","year":"2016","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1038\/nature742","article-title":"Molecular identification of a renal urate-anion exchanger that regulates blood urate levels","volume":"417","author":"Enomoto","year":"2002","journal-title":"Nature"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/2\/1153\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:49:15Z","timestamp":1760104155000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/2\/1153"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,18]]},"references-count":28,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,1]]}},"alternative-id":["ijms25021153"],"URL":"https:\/\/doi.org\/10.3390\/ijms25021153","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,18]]}}}