{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T02:33:00Z","timestamp":1792290780216,"version":"4.4.0"},"reference-count":31,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T00:00:00Z","timestamp":1705536000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"State Assignment No. 10\/21 (FBRI SRC VB \u2018Vector\u2019 Rospotrebnadzor)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Tumor necrosis factor alpha (TNF-\u03b1) is a cytokine that is responsible for many processes associated with immune response and inflammation. It is involved in the development of an antiviral response to many virus infections. This factor was shown to be activated in influenza A virus infection, which enhances production of other cytokines. The overexpression of these cytokines can lead to a cytokine storm. To study the role of TNF-\u03b1 in the development of pathologies associated with viral infection, we generated a Tnfa knockout mouse strain. We demonstrated that these mice were characterized by a significant increase in the number of viral genomes compared to that in the parental strain, but the amount of live virus did not differ. A histopathology of the lungs in the genetically modified animals was significantly lower in terms of interalveolar septal infiltration. The generated model may be used to further study pathological processes in viral infections.<\/jats:p>","DOI":"10.3390\/ijms25021156","type":"journal-article","created":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T03:50:56Z","timestamp":1705549856000},"page":"1156","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Knockout of the Tnfa Gene Decreases Influenza Virus-Induced Histological Reactions in Laboratory Mice"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-1335-6249","authenticated-orcid":false,"given":"Darya A.","family":"Savenkova","sequence":"first","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u201cVector\u201d, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being (FBRI SRC VB \u201cVector\u201d, Rospotrebnadzor), Koltsovo 630559, Russia"},{"name":"Novosibirsk State University, Pirogova 2, Novosibirsk 630090, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6952-6412","authenticated-orcid":false,"given":"Andrey S.","family":"Gudymo","sequence":"additional","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u201cVector\u201d, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being (FBRI SRC VB \u201cVector\u201d, Rospotrebnadzor), Koltsovo 630559, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey N.","family":"Korablev","sequence":"additional","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u201cVector\u201d, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being (FBRI SRC VB \u201cVector\u201d, Rospotrebnadzor), Koltsovo 630559, Russia"},{"name":"Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Science, Lavrentieva 10, Novosibirsk 630090, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6746-8092","authenticated-orcid":false,"given":"Oleg S.","family":"Taranov","sequence":"additional","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u201cVector\u201d, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being (FBRI SRC VB \u201cVector\u201d, Rospotrebnadzor), Koltsovo 630559, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5786-7965","authenticated-orcid":false,"given":"Darya V.","family":"Bazovkina","sequence":"additional","affiliation":[{"name":"Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Science, Lavrentieva 10, Novosibirsk 630090, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nataliya V.","family":"Danilchenko","sequence":"additional","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u201cVector\u201d, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being (FBRI SRC VB \u201cVector\u201d, Rospotrebnadzor), Koltsovo 630559, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga N.","family":"Perfilyeva","sequence":"additional","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u201cVector\u201d, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being (FBRI SRC VB \u201cVector\u201d, Rospotrebnadzor), Koltsovo 630559, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena K.","family":"Ivleva","sequence":"additional","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u201cVector\u201d, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being (FBRI SRC VB \u201cVector\u201d, Rospotrebnadzor), Koltsovo 630559, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasiya A.","family":"Moiseeva","sequence":"additional","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u201cVector\u201d, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being (FBRI SRC VB \u201cVector\u201d, Rospotrebnadzor), Koltsovo 630559, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulia A.","family":"Bulanovich","sequence":"additional","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u201cVector\u201d, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being (FBRI SRC VB \u201cVector\u201d, Rospotrebnadzor), Koltsovo 630559, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena V.","family":"Roshchina","sequence":"additional","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u201cVector\u201d, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being (FBRI SRC VB \u201cVector\u201d, Rospotrebnadzor), Koltsovo 630559, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina A.","family":"Serova","sequence":"additional","affiliation":[{"name":"Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Science, Lavrentieva 10, Novosibirsk 630090, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0611-0203","authenticated-orcid":false,"given":"Nariman R.","family":"Battulin","sequence":"additional","affiliation":[{"name":"Novosibirsk State University, Pirogova 2, Novosibirsk 630090, Russia"},{"name":"Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Science, Lavrentieva 10, Novosibirsk 630090, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9102-1525","authenticated-orcid":false,"given":"Elizabeth A.","family":"Kulikova","sequence":"additional","affiliation":[{"name":"Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Science, Lavrentieva 10, Novosibirsk 630090, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8940-9173","authenticated-orcid":false,"given":"Dmitry V.","family":"Yudkin","sequence":"additional","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u201cVector\u201d, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being (FBRI SRC VB \u201cVector\u201d, Rospotrebnadzor), Koltsovo 630559, 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