{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T05:15:42Z","timestamp":1771305342946,"version":"3.50.1"},"reference-count":39,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,20]],"date-time":"2024-01-20T00:00:00Z","timestamp":1705708800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Instituto de Salud Carlos III, ISCIII and the European Regional Development Fund, ERDF","award":["PI081449"],"award-info":[{"award-number":["PI081449"]}]},{"name":"Instituto de Salud Carlos III, ISCIII and the European Regional Development Fund, ERDF","award":["GPC2014\/036"],"award-info":[{"award-number":["GPC2014\/036"]}]},{"name":"Instituto de Salud Carlos III, ISCIII and the European Regional Development Fund, ERDF","award":["ED341b 2017\/19"],"award-info":[{"award-number":["ED341b 2017\/19"]}]},{"name":"Instituto de Salud Carlos III, ISCIII and the European Regional Development Fund, ERDF","award":["ED431B 2020\/37"],"award-info":[{"award-number":["ED431B 2020\/37"]}]},{"DOI":"10.13039\/501100010801","name":"Xunta de Galicia","doi-asserted-by":"publisher","award":["PI081449"],"award-info":[{"award-number":["PI081449"]}],"id":[{"id":"10.13039\/501100010801","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010801","name":"Xunta de Galicia","doi-asserted-by":"publisher","award":["GPC2014\/036"],"award-info":[{"award-number":["GPC2014\/036"]}],"id":[{"id":"10.13039\/501100010801","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010801","name":"Xunta de Galicia","doi-asserted-by":"publisher","award":["ED341b 2017\/19"],"award-info":[{"award-number":["ED341b 2017\/19"]}],"id":[{"id":"10.13039\/501100010801","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010801","name":"Xunta de Galicia","doi-asserted-by":"publisher","award":["ED431B 2020\/37"],"award-info":[{"award-number":["ED431B 2020\/37"]}],"id":[{"id":"10.13039\/501100010801","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Asociaci\u00f3n Espa\u00f1ola de Familiares y Afectados de Lipodistrofias (AELIP)","award":["PI081449"],"award-info":[{"award-number":["PI081449"]}]},{"name":"Asociaci\u00f3n Espa\u00f1ola de Familiares y Afectados de Lipodistrofias (AELIP)","award":["GPC2014\/036"],"award-info":[{"award-number":["GPC2014\/036"]}]},{"name":"Asociaci\u00f3n Espa\u00f1ola de Familiares y Afectados de Lipodistrofias (AELIP)","award":["ED341b 2017\/19"],"award-info":[{"award-number":["ED341b 2017\/19"]}]},{"name":"Asociaci\u00f3n Espa\u00f1ola de Familiares y Afectados de Lipodistrofias (AELIP)","award":["ED431B 2020\/37"],"award-info":[{"award-number":["ED431B 2020\/37"]}]},{"name":"Fundaci\u00f3n Alfonso Mart\u00edn Escudero","award":["PI081449"],"award-info":[{"award-number":["PI081449"]}]},{"name":"Fundaci\u00f3n Alfonso Mart\u00edn Escudero","award":["GPC2014\/036"],"award-info":[{"award-number":["GPC2014\/036"]}]},{"name":"Fundaci\u00f3n Alfonso Mart\u00edn Escudero","award":["ED341b 2017\/19"],"award-info":[{"award-number":["ED341b 2017\/19"]}]},{"name":"Fundaci\u00f3n Alfonso Mart\u00edn Escudero","award":["ED431B 2020\/37"],"award-info":[{"award-number":["ED431B 2020\/37"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The accumulation of farnesylated prelamin A has been suggested as one of the mechanisms responsible for the loss of fat in type 2 familial partial lipodystrophy due to variants in the LMNA gene. In this rare disease, fat loss appears in women after puberty, affecting sex-hormone-dependent anatomical areas. This study investigated the impact of 17-\u03b2-estradiol on adipogenesis in murine preadipocytes subjected to a pharmacologically induced accumulation of farnesylated and non-farnesylated prelamin A. To induce the accumulation of non-farnesylated or farnesylated prelamin A, 3T3-L1 cells were treated with the farnesyltransferase inhibitor 277 or the methyltransferase inhibitor N-acetyl-S-farnesyl-l-cysteine methylester. Subsequently, the cells were induced to undergo adipocyte differentiation in the presence or absence of 17-\u03b2-estradiol. Prelamin A accumulation was assessed through immunofluorescence, while real-time PCR and Western blot techniques were used to quantify several adipogenic genes and evaluate protein levels, respectively. The results showed that 17-\u03b2-estradiol increased adipogenesis, although the combination of this hormone plus farnesylated prelamin A led to a reduction in the number of mature adipocytes and the expression of the different genes involved in adipogenesis. In conclusion, the influence of farnesylated prelamin A accumulation on adipogenesis manifested only in the presence of estradiol. These in vitro findings suggest a potential mechanism that could explain the characteristic phenotype in women suffering type 2 familial partial lipodystrophy.<\/jats:p>","DOI":"10.3390\/ijms25021282","type":"journal-article","created":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T03:50:22Z","timestamp":1705895422000},"page":"1282","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of \u03b2-Estradiol on Adipogenesis in a 3T3-L1 Cell Model of Prelamin A Accumulation"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0941-626X","authenticated-orcid":false,"given":"Silvia","family":"Cobelo-G\u00f3mez","sequence":"first","affiliation":[{"name":"Thyroid and Metabolic Diseases Unit (U.E.T.eM.), Department of Psychiatry, Radiology, Public Health, Nursing and Medicine, Center for Research in Molecular Medicine and Chronic Diseases (CIMUS)-IDIS, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3887-0573","authenticated-orcid":false,"given":"Sof\u00eda","family":"S\u00e1nchez-Iglesias","sequence":"additional","affiliation":[{"name":"Thyroid and Metabolic Diseases Unit (U.E.T.eM.), Department of Psychiatry, Radiology, Public Health, Nursing and Medicine, Center for Research in Molecular Medicine and Chronic Diseases (CIMUS)-IDIS, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1268-7538","authenticated-orcid":false,"given":"Ant\u00eda","family":"Fern\u00e1ndez-Pombo","sequence":"additional","affiliation":[{"name":"Thyroid and Metabolic Diseases Unit (U.E.T.eM.), Department of Psychiatry, Radiology, Public Health, Nursing and Medicine, Center for Research in Molecular Medicine and Chronic Diseases (CIMUS)-IDIS, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2852-7851","authenticated-orcid":false,"given":"David","family":"Ara\u00fajo-Vilar","sequence":"additional","affiliation":[{"name":"Thyroid and Metabolic Diseases Unit (U.E.T.eM.), Department of Psychiatry, Radiology, Public Health, Nursing and Medicine, Center for Research in Molecular Medicine and Chronic Diseases (CIMUS)-IDIS, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0002-9343(99)00414-3","article-title":"Lipodystrophies","volume":"108","author":"Garg","year":"2000","journal-title":"Am. 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