{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:22:22Z","timestamp":1789179742095,"version":"build-2803163510"},"reference-count":62,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,20]],"date-time":"2024-01-20T00:00:00Z","timestamp":1705708800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Innovation Platform for Horticultural Biotechnology Genetic Transfusion, Fujian Academy of Agricultural Sciences","award":["CXPT202204"],"award-info":[{"award-number":["CXPT202204"]}]},{"name":"the Innovation Platform for Horticultural Biotechnology Genetic Transfusion, Fujian Academy of Agricultural Sciences","award":["2020-3501-04-001995"],"award-info":[{"award-number":["2020-3501-04-001995"]}]},{"name":"Enterprise Technology Development","award":["CXPT202204"],"award-info":[{"award-number":["CXPT202204"]}]},{"name":"Enterprise Technology Development","award":["2020-3501-04-001995"],"award-info":[{"award-number":["2020-3501-04-001995"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This study explores the impact of RNAi in terms of selectively inhibiting the expression of the OsBBTI5 gene, with the primary objective of uncovering its involvement in the molecular mechanisms associated with salt tolerance in rice. OsBBTI5, belonging to the Bowman\u2013Birk inhibitor (BBI) family gene, is known for its involvement in plant stress responses. The gene was successfully cloned from rice, exhibiting transcriptional self-activation in yeast. A yeast two-hybrid assay confirmed its specific binding to OsAPX2 (an ascorbate peroxidase gene). Transgenic OsBBTI5-RNAi plants displayed insensitivity to varying concentrations of 24-epibrassinolide in the brassinosteroid sensitivity assay. However, they showed reduced root and plant height at high concentrations (10 and 100 \u00b5M) of GA3 immersion. Enzyme activity assays revealed increased peroxidase (POD) and superoxide dismutase (SOD) activities and decreased malondialdehyde (MDA) content under 40-60 mM NaCl. Transcriptomic analysis indicated a significant upregulation of photosynthesis-related genes in transgenic plants under salt stress compared to the wild type. Notably, this study provides novel insights, suggesting that the BBI gene is part of the BR signaling pathway, and that OsBBTI5 potentially enhances stress tolerance in transgenic plants through interaction with the salt stress-related gene OsAPX2.<\/jats:p>","DOI":"10.3390\/ijms25021284","type":"journal-article","created":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T03:50:22Z","timestamp":1705895422000},"page":"1284","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["RNAi-Mediated Suppression of OsBBTI5 Promotes Salt Stress Tolerance in Rice"],"prefix":"10.3390","volume":"25","author":[{"given":"Zhimin","family":"Lin","sequence":"first","affiliation":[{"name":"Fujian Academy of Agricultural Sciences Biotechnology Institute, Fuzhou 350003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Yi","sequence":"additional","affiliation":[{"name":"College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3092-6328","authenticated-orcid":false,"given":"Muhammad Moaaz","family":"Ali","sequence":"additional","affiliation":[{"name":"College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaojuan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengnan","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9655-5868","authenticated-orcid":false,"given":"Faxing","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Nadarajah, K., Abdul Hamid, N.W., and Abdul Rahman, N.S.N. 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