{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T07:22:28Z","timestamp":1773386548835,"version":"3.50.1"},"reference-count":65,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,20]],"date-time":"2024-01-20T00:00:00Z","timestamp":1705708800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Conahcyt","doi-asserted-by":"publisher","award":["581720"],"award-info":[{"award-number":["581720"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Conahcyt","doi-asserted-by":"publisher","award":["CB-A1-S-8989"],"award-info":[{"award-number":["CB-A1-S-8989"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Conahcyt, Mexico","doi-asserted-by":"publisher","award":["581720"],"award-info":[{"award-number":["581720"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Conahcyt, Mexico","doi-asserted-by":"publisher","award":["CB-A1-S-8989"],"award-info":[{"award-number":["CB-A1-S-8989"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Respiratory diseases in ruminants are a main cause of economic losses to farmers worldwide. Approximately 25% of ruminants experience at least one episode of respiratory disease during the first year of life. Mannheimia haemolytica is the main etiological bacterial agent in the ruminant respiratory disease complex. M. haemolytica can secrete several virulence factors, such as leukotoxin, lipopolysaccharide, and proteases, that can be targeted to treat infections. At present, little information has been reported on the secretion of M. haemolytica A2 proteases and their host protein targets. Here, we obtained evidence that M. haemolytica A2 proteases promote the degradation of hemoglobin, holo-lactoferrin, albumin, and fibrinogen. Additionally, we performed biochemical characterization for a specific 110 kDa Zn-dependent metalloprotease (110-Mh metalloprotease). This metalloprotease was purified through ion exchange chromatography and characterized using denaturing and chaotropic agents and through zymography assays. Furthermore, mass spectrometry identification and 3D modeling were performed. Then, antibodies against the 110 kDa-Mh metalloprotease were produced, which achieved great inhibition of proteolytic activity. Finally, the antibodies were used to perform immunohistochemical tests on postmortem lung samples from sheep with suggestive histology data of pneumonic mannheimiosis. Taken together, our results strongly suggest that the 110-Mh metalloprotease participates as a virulence mechanism that promotes damage to host tissues.<\/jats:p>","DOI":"10.3390\/ijms25021289","type":"journal-article","created":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T03:50:22Z","timestamp":1705895422000},"page":"1289","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Identification, Biochemical Characterization, and In Vivo Detection of a Zn-Metalloprotease with Collagenase Activity from Mannheimia haemolytica A2"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2546-2792","authenticated-orcid":false,"given":"Gerardo","family":"Ram\u00edrez-Rico","sequence":"first","affiliation":[{"name":"Faculty of Professional Studies Cuautitlan, Autonomous National University of Mexico (UNAM), Mexico City 54714, Mexico"},{"name":"Department of Cell Biology, Center for Research and Advanced Studies, Mexico City 07360, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9569-0064","authenticated-orcid":false,"given":"Moises","family":"Martinez-Castillo","sequence":"additional","affiliation":[{"name":"Liver, Pancreas and Motility Laboratory, Unit of Research in Experimental Medicine, School of Medicine, Autonomous National University of Mexico (UNAM), Mexico City 06726, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucero","family":"Ruiz-Maz\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Center for Research and Advanced Studies, Mexico City 07360, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4897-1118","authenticated-orcid":false,"given":"Erika Patricia","family":"Meneses-Romero","sequence":"additional","affiliation":[{"name":"Laboratory of Proteomics, Biotechnology Institute IBT-UNAM, Cuernavaca 62210, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Arturo Flores","family":"Palacios","sequence":"additional","affiliation":[{"name":"Hayko Scientific Laboratories, Mexico City 14420, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Efr\u00e9n","family":"D\u00edaz-Aparicio","sequence":"additional","affiliation":[{"name":"National Center for Disciplinary Research in Animal Health and Safety, National Institute of Forestry, Agricultural and Livestock Research (INIFAP), Mexico City 05110, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4362-8044","authenticated-orcid":false,"given":"Erasmo Negrete","family":"Abascal","sequence":"additional","affiliation":[{"name":"Faculty of Professional Studies Iztacala, Autonomous National University of Mexico (UNAM), Mexico City 54090, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mireya","family":"de la Garza","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Center for Research and Advanced Studies, Mexico City 07360, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.vetmic.2016.11.012","article-title":"Mannheimia haemolytica biofilm formation on bovine respiratory epithelial cells","volume":"197","author":"Boukahil","year":"2016","journal-title":"Vet. 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