{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T18:22:12Z","timestamp":1785522132994,"version":"3.56.0"},"reference-count":63,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,1,26]],"date-time":"2024-01-26T00:00:00Z","timestamp":1706227200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"National Science Centre in Poland","doi-asserted-by":"publisher","award":["2021\/43\/B\/NZ9\/00204"],"award-info":[{"award-number":["2021\/43\/B\/NZ9\/00204"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The increasing incidence of male infertility in humans and animals creates the need to search for new factors that significantly affect the course of reproductive processes. Therefore, the aim of this study was to determine the temporospatial expression of aquaglyceroporins (AQP3, AQP7 and AQP9) in the bovine (Bos taurus) reproductive system using immunohistochemistry and Western blotting. The study also included morphological analysis and identification of GATA-4. In brief, in immature individuals, AQP3 and AQP7 were found in gonocytes. In reproductive bulls, AQP3 was observed in spermatocytes and spermatogonia, while AQP7 was visible in all germ cells and the Sertoli cells. AQP7 and AQP9 were detected in the Leydig cells. Along the entire epididymis of reproductive bulls, aquaglyceroporins were visible, among others, in basal cells (AQP3 and AQP7), in epididymal sperm (AQP7) and in the stereocilia of the principal cells (AQP9). In males of all ages, aquaglyceroporins were identified in the principal and basal cells of the vas deferens. An increase in the expression of AQP3 in the testis and cauda epididymis and a decrease in the abundance of AQP7 in the vas deferens with age were found. In conclusion, age-related changes in the expression and\/or distribution patterns of AQP3, AQP7 and AQP9 indicate the involvement of these proteins in the normal development and course of male reproductive processes in cattle.<\/jats:p>","DOI":"10.3390\/ijms25031567","type":"journal-article","created":{"date-parts":[[2024,1,26]],"date-time":"2024-01-26T10:54:27Z","timestamp":1706266467000},"page":"1567","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Cellular Distribution of Aquaporin 3, 7 and 9 in the Male Reproductive System: A Lesson from Bovine Study (Bos taurus)"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8953-3679","authenticated-orcid":false,"given":"Patrycja","family":"Oberska","sequence":"first","affiliation":[{"name":"Department of Physiology, Cytobiology and Proteomics, West Pomeranian University of Technology in Szczecin, Klemensa Janickiego 29, 71-270 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0861-0738","authenticated-orcid":false,"given":"Marta","family":"Grabowska","sequence":"additional","affiliation":[{"name":"Department of Histology and Developmental Biology, Pomeranian Medical University, \u017bo\u0142nierska 48, 71-210 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Marynowska","sequence":"additional","affiliation":[{"name":"Department of Physiology, Cytobiology and Proteomics, West Pomeranian University of Technology in Szczecin, Klemensa Janickiego 29, 71-270 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0720-7755","authenticated-orcid":false,"given":"Maciej","family":"Murawski","sequence":"additional","affiliation":[{"name":"Department of Nutrition, Animal Biotechnology and Fisheries, University of Agriculture in Krakow, 24\/28 Mickiewicza Avenue, 30-059 Cracow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9121-4498","authenticated-orcid":false,"given":"Dariusz","family":"G\u0105czarzewicz","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, Biotechnology and Environmental Hygiene, West Pomeranian University of Technology in Szczecin, Klemensa Janickiego 29, 71-270 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Syczewski","sequence":"additional","affiliation":[{"name":"Genetic and Animal Husbandry, Tomasza 40, 72-006 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7201-328X","authenticated-orcid":false,"given":"Katarzyna","family":"Micha\u0142ek","sequence":"additional","affiliation":[{"name":"Department of Physiology, Cytobiology and Proteomics, West Pomeranian University of Technology in Szczecin, Klemensa Janickiego 29, 71-270 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1006\/bbrc.2001.5810","article-title":"Possible Involvement of Aquaporin-7 and -8 in Rat Testis Development and Spermatogenesis","volume":"288","author":"Calamita","year":"2001","journal-title":"Biochem. 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