{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T18:17:37Z","timestamp":1785608257364,"version":"3.56.0"},"reference-count":55,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,1,30]],"date-time":"2024-01-30T00:00:00Z","timestamp":1706572800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Academician (Expert) Workstation of Yunnan Province Program","award":["202305AF150127"],"award-info":[{"award-number":["202305AF150127"]}]},{"name":"Academician (Expert) Workstation of Yunnan Province Program","award":["YNWR-CYJS-2019-020"],"award-info":[{"award-number":["YNWR-CYJS-2019-020"]}]},{"name":"Academician (Expert) Workstation of Yunnan Province Program","award":["2022YFD1601900"],"award-info":[{"award-number":["2022YFD1601900"]}]},{"name":"Academician (Expert) Workstation of Yunnan Province Program","award":["202301AT070488"],"award-info":[{"award-number":["202301AT070488"]}]},{"name":"Yunnan Ten Thousand Talents Plan Leading Talents of Industrial Technology Project of China","award":["202305AF150127"],"award-info":[{"award-number":["202305AF150127"]}]},{"name":"Yunnan Ten Thousand Talents Plan Leading Talents of Industrial Technology Project of China","award":["YNWR-CYJS-2019-020"],"award-info":[{"award-number":["YNWR-CYJS-2019-020"]}]},{"name":"Yunnan Ten Thousand Talents Plan Leading Talents of Industrial Technology Project of China","award":["2022YFD1601900"],"award-info":[{"award-number":["2022YFD1601900"]}]},{"name":"Yunnan Ten Thousand Talents Plan Leading Talents of Industrial Technology Project of China","award":["202301AT070488"],"award-info":[{"award-number":["202301AT070488"]}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["202305AF150127"],"award-info":[{"award-number":["202305AF150127"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["YNWR-CYJS-2019-020"],"award-info":[{"award-number":["YNWR-CYJS-2019-020"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD1601900"],"award-info":[{"award-number":["2022YFD1601900"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["202301AT070488"],"award-info":[{"award-number":["202301AT070488"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Yunnan Fundamental Research Projects","award":["202305AF150127"],"award-info":[{"award-number":["202305AF150127"]}]},{"name":"Yunnan Fundamental Research Projects","award":["YNWR-CYJS-2019-020"],"award-info":[{"award-number":["YNWR-CYJS-2019-020"]}]},{"name":"Yunnan Fundamental Research Projects","award":["2022YFD1601900"],"award-info":[{"award-number":["2022YFD1601900"]}]},{"name":"Yunnan Fundamental Research Projects","award":["202301AT070488"],"award-info":[{"award-number":["202301AT070488"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Hydropericardium hepatitis syndrome (HHS) is primarily caused by fowl adenovirus serotype 4 (FAdV-4), causing high mortality in chickens. Although vaccination strategies against FAdV-4 have been adopted, HHS still occurs sporadically. Furthermore, no effective drugs are available for controlling FAdV-4 infection. However, type I and III interferon (IFN) are crucial therapeutic agents against viral infection. The following experiments were conducted to investigate the inhibitory effect of chicken IFN against FadV-4. We expressed recombinant chicken type I IFN-\u03b1 (ChIFN-\u03b1) and type III IFN-\u03bb (ChIFN-\u03bb) in Escherichia coli and systemically investigated their antiviral activity against FAdV-4 infection in Leghorn male hepatocellular (LMH) cells. ChIFN-\u03b1 and ChIFN-\u03bb dose dependently inhibited FAdV-4 replication in LMH cells. Compared with ChIFN-\u03bb, ChIFN-\u03b1 more significantly inhibited viral genome transcription but less significantly suppressed FAdV-4 release. ChIFN-\u03b1- and ChIFN-\u03bb-induced IFN-stimulated gene (ISG) expression, such as PKR, ZAP, IRF7, MX1, Viperin, IFIT5, OASL, and IFI6, in LMH cells; however, ChIFN-\u03b1 induced a stronger expression level than ChIFN-\u03bb. Thus, our data revealed that ChIFN-\u03b1 and ChIFN-\u03bb might trigger different ISG expression levels, inhibiting FAdV-4 replication via different steps of the FAdV-4 lifecycle, which furthers the potential applications of IFN antiviral drugs in chickens.<\/jats:p>","DOI":"10.3390\/ijms25031681","type":"journal-article","created":{"date-parts":[[2024,1,30]],"date-time":"2024-01-30T03:45:12Z","timestamp":1706586312000},"page":"1681","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Chicken Interferon-Alpha and -Lambda Exhibit Antiviral Effects against Fowl Adenovirus Serotype 4 in Leghorn Male Hepatocellular Cells"],"prefix":"10.3390","volume":"25","author":[{"given":"Jinyu","family":"Lai","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"},{"name":"College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingchen","family":"He","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"},{"name":"Center for Poultry Disease Control and Prevention, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongjie","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"},{"name":"Center for Poultry Disease Control and Prevention, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Limei","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"},{"name":"Center for Poultry Disease Control and Prevention, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Libin","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengping","family":"He","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"},{"name":"Center for Poultry Disease Control and Prevention, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"},{"name":"Center for Poultry Disease Control and Prevention, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangyu","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"},{"name":"Center for Poultry Disease Control and Prevention, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Ren","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Xiang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"},{"name":"Center for Poultry Disease Control and Prevention, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e103","DOI":"10.1038\/emi.2017.91","article-title":"Isolation and molecular characterization of prevalent Fowl adenovirus strains in southwestern China during 2015\u20132016 for the development of a control strategy","volume":"6","author":"Xia","year":"2017","journal-title":"Emerg. 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