{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T15:03:02Z","timestamp":1773154982200,"version":"3.50.1"},"reference-count":42,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIGMS","doi-asserted-by":"publisher","award":["1SC2GM144188-01"],"award-info":[{"award-number":["1SC2GM144188-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIGMS","doi-asserted-by":"publisher","award":["1U54MD012392"],"award-info":[{"award-number":["1U54MD012392"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIGMS","doi-asserted-by":"publisher","award":["P30ES025128"],"award-info":[{"award-number":["P30ES025128"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institute of Minority Health Disparities (NIMHD)","doi-asserted-by":"publisher","award":["1SC2GM144188-01"],"award-info":[{"award-number":["1SC2GM144188-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institute of Minority Health Disparities (NIMHD)","doi-asserted-by":"publisher","award":["1U54MD012392"],"award-info":[{"award-number":["1U54MD012392"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institute of Minority Health Disparities (NIMHD)","doi-asserted-by":"publisher","award":["P30ES025128"],"award-info":[{"award-number":["P30ES025128"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institute of Environmental Health Sciences (NIEHS)","doi-asserted-by":"publisher","award":["1SC2GM144188-01"],"award-info":[{"award-number":["1SC2GM144188-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institute of Environmental Health Sciences (NIEHS)","doi-asserted-by":"publisher","award":["1U54MD012392"],"award-info":[{"award-number":["1U54MD012392"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institute of Environmental Health Sciences (NIEHS)","doi-asserted-by":"publisher","award":["P30ES025128"],"award-info":[{"award-number":["P30ES025128"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Here we use the SCIREQ InExpose system to simulate a biologically relevant vaping model in mice to investigate the role of calcium signaling in vape-dependent pulmonary disease as well as to investigate if there is a gender-based difference of disease. Male and female mice were vaped with JUUL Menthol (3% nicotine) using the SCIREQ InExpose system for 2 weeks. Additionally, 2-APB, a known calcium signaling inhibitor, was administered as a prophylactic for lung disease and damage caused by vaping. After 2 weeks, mice were exposed to lipopolysaccharide (LPS) to mimic a bacterial infection. Post-infection (24 h), mice were sacrificed, and bronchoalveolar lavage fluid (BALF) and lungs were taken. Vaping primed the lungs for worsened disease burden after microbial challenge (LPS) for both males and females, though females presented increased neutrophilia and inflammatory cytokines post-vape compared to males, which was assessed by flow cytometry, and cytokine and histopathological analysis. This increased inflammatory burden was controlled by calcium signaling inhibition, suggesting that calcium dysregulation may play a role in lung injury caused by vaping in a gender-dependent manner.<\/jats:p>","DOI":"10.3390\/ijms25031785","type":"journal-article","created":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T08:44:08Z","timestamp":1706777048000},"page":"1785","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Vaping-Dependent Pulmonary Inflammation Is Ca2+ Mediated and Potentially Sex Specific"],"prefix":"10.3390","volume":"25","author":[{"given":"Jeffrey G.","family":"Shipman","sequence":"first","affiliation":[{"name":"Department of Biological & Biomedical Sciences, North Carolina Central University, Durham, NC 27707, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rob U.","family":"Onyenwoke","sequence":"additional","affiliation":[{"name":"Department of Biological & Biomedical Sciences, North Carolina Central University, Durham, NC 27707, USA"},{"name":"Biomanufacturing Research Institute and Technology Enterprise (BRITE), North Carolina Central University, Durham, NC 27707, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9848-6640","authenticated-orcid":false,"given":"Vijay","family":"Sivaraman","sequence":"additional","affiliation":[{"name":"Department of Biological & Biomedical Sciences, North Carolina Central University, Durham, NC 27707, USA"},{"name":"The Julius L. Chambers Biomedical\/Biotechnology Research Institute, North Carolina Central University, Durham, NC 27707, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,1]]},"reference":[{"key":"ref_1","unstructured":"GBD 2019 Chronic Respiratory Diseases Collaborators (2023). Global burden of chronic respiratory diseases and risk factors, 1990\u20132019: An update from the Global Burden of Disease Study 2019. eClinicalMedicine, 59, 101936."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.1164\/ajrccm.163.6.2004035","article-title":"Alveolar fluid clearance is impaired in the majority of patients with acute lung injury and the acute respiratory distress syndrome","volume":"163","author":"Ware","year":"2001","journal-title":"Am. J. Respir. Crit. 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