{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T13:57:22Z","timestamp":1762005442482,"version":"build-2065373602"},"reference-count":68,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,2,6]],"date-time":"2024-02-06T00:00:00Z","timestamp":1707177600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science, Technological Development and Innovations of the Republic of Serbia","award":["175043","200110"],"award-info":[{"award-number":["175043","200110"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The aim of this study was to investigate the effects of aerobic treadmill training regimen of four weeks duration on oxidative stress parameters, metabolic enzymes, and histomorphometric changes in the colon of hyperhomocysteinemic rats. Male Wistar albino rats were divided into four groups (n = 10, per group): C, 0.9% NaCl 0.2 mL\/day subcutaneous injection (s.c.) 2x\/day; H, homocysteine 0.45 \u00b5mol\/g b.w.\/day s.c. 2x\/day; CPA, saline (0.9% NaCl 0.2 mL\/day s.c. 2x\/day) and an aerobic treadmill training program; and HPA, homocysteine (0.45 \u00b5mol\/g b.w.\/day s.c. 2x\/day) and an aerobic treadmill training program. The HPA group had an increased level of malondialdehyde (5.568 \u00b1 0.872 \u03bcmol\/mg protein, p = 0.0128 vs. CPA (3.080 \u00b1 0.887 \u03bcmol\/mg protein)), catalase activity (3.195 \u00b1 0.533 U\/mg protein, p &lt; 0.0001 vs. C (1.467 \u00b1 0.501 U\/mg protein), p = 0.0012 vs. H (1.955 \u00b1 0.293 U\/mg protein), and p = 0.0003 vs. CPA (1.789 \u00b1 0.256 U\/mg protein)), and total superoxide dismutase activity (9.857 \u00b1 1.566 U\/mg protein, p &lt; 0.0001 vs. C (6.738 \u00b1 0.339 U\/mg protein), p &lt; 0.0001 vs. H (6.015 \u00b1 0.424 U\/mg protein), and p &lt; 0.0001 vs. CPA (5.172 \u00b1 0.284 U\/mg protein)) were detected in the rat colon. In the HPA group, higher activities of lactate dehydrogenase (2.675 \u00b1 1.364 mU\/mg protein) were detected in comparison to the CPA group (1.198 \u00b1 0.217 mU\/mg protein, p = 0.0234) and higher activities of malate dehydrogenase (9.962 (5.752\u201310.220) mU\/mg protein) were detected in comparison to the CPA group (4.727 (4.562\u20135.299) mU\/mg protein, p = 0.0385). Subchronic treadmill training in the rats with hyperhomocysteinemia triggers the colon tissue antioxidant response (by increasing the activities of superoxide dismutase and catalase) and elicits an increase in metabolic enzyme activities (lactate dehydrogenase and malate dehydrogenase). This study offers a comprehensive assessment of the effects of aerobic exercise on colonic tissues in a rat model of hyperhomocysteinemia, evaluating a range of biological indicators including antioxidant enzyme activity, metabolic enzyme activity, and morphometric parameters, which suggested that exercise may confer protective effects at both the physiological and morphological levels.<\/jats:p>","DOI":"10.3390\/ijms25041946","type":"journal-article","created":{"date-parts":[[2024,2,6]],"date-time":"2024-02-06T03:22:31Z","timestamp":1707189751000},"page":"1946","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effects of Aerobic Treadmill Training on Oxidative Stress Parameters, Metabolic Enzymes, and Histomorphometric Changes in Colon of Rats with Experimentally Induced Hyperhomocysteinemia"],"prefix":"10.3390","volume":"25","author":[{"given":"Marija","family":"Stojanovi\u0107","sequence":"first","affiliation":[{"name":"Institute of Medical Physiology \u201cRichard Burian\u201d, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8270-3089","authenticated-orcid":false,"given":"Du\u0161an","family":"Todorovi\u0107","sequence":"additional","affiliation":[{"name":"Institute of Medical Physiology \u201cRichard Burian\u201d, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristina","family":"Gop\u010devi\u0107","sequence":"additional","affiliation":[{"name":"Institute of Chemistry in Medicine \u201cPetar Matavulj\u201d, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Medi\u0107","sequence":"additional","affiliation":[{"name":"Institute of Chemistry in Medicine \u201cPetar Matavulj\u201d, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milica","family":"Labudovi\u0107 Borovi\u0107","sequence":"additional","affiliation":[{"name":"Institute of Histology and Embryology \u201cAleksandar \u00d0. Kosti\u0107\u201d, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8283-4815","authenticated-orcid":false,"given":"Sanja","family":"Despotovi\u0107","sequence":"additional","affiliation":[{"name":"Institute of Histology and Embryology \u201cAleksandar \u00d0. Kosti\u0107\u201d, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2196-9438","authenticated-orcid":false,"given":"Dragan","family":"Djuric","sequence":"additional","affiliation":[{"name":"Institute of Medical Physiology \u201cRichard Burian\u201d, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"\u0160kovierov\u00e1, H., Vidomanov\u00e1, E., Mahmood, S., Sopkov\u00e1, J., Drgov\u00e1, A., \u010cerve\u0148ov\u00e1, T., Hala\u0161ov\u00e1, E., and Lehotsk\u00fd, J. (2016). The Molecular and Cellular Effect of Homocysteine Metabolism Imbalance on Human Health. Int. J. Mol. 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