{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T06:18:02Z","timestamp":1788848282567,"version":"build-2803163510"},"reference-count":62,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,2,9]],"date-time":"2024-02-09T00:00:00Z","timestamp":1707436800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Doctoral School of the University of Medicine and Pharmacy of Craiova, Romania","award":["26\/12C\/13.07.2021"],"award-info":[{"award-number":["26\/12C\/13.07.2021"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Diabetes mellitus is a metabolic disorder with global economic implications that can lead to complications such as diabetic cardiomyopathy. The aim of this study was to compare the effects of chitosan versus dapagliflozin in mouse diabetic cardiomyopathy. We used 32 C57Bl\/6 male mice aged between 8 and 10 weeks, which were randomly divided into Control\u2014without diabetes mellitus (DM), type 1 DM (T1DM), T1DM + Chitosan, and T1DM + Dapapgliflozin groups. We induced diabetes with streptozotocin and treated the animals for 12 weeks. The analysis showed a reduction in intramyocardial fibrosis in the T1DM + Dapapgliflozin compared to T1DM animals. In T1DM + CHIT, a reduction in intramyocardial fibrosis was observed although, accordingly, there was also no significant decrease in blood glucose. The level of oxidative stress was reduced in the groups of treated animals compared to T1DM. All these observed changes in the structure and function of hearts were highlighted in the echocardiographic examination. In the treated groups, there was delayed appearance of left ventricular (LV) hypertrophy, a slight decrease in the ejection fraction of the LV, and an improved diastolic profile. The results demonstrate that chitosan has promising effects on diabetic cardiomyopathy that are comparable to the beneficial effects of dapagliflozin.<\/jats:p>","DOI":"10.3390\/ijms25042118","type":"journal-article","created":{"date-parts":[[2024,2,9]],"date-time":"2024-02-09T09:04:01Z","timestamp":1707469441000},"page":"2118","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Chitosan Versus Dapagliflozin in a Diabetic Cardiomyopathy Mouse Model"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7205-306X","authenticated-orcid":false,"given":"Georgic\u0103","family":"T\u00e2rtea","sequence":"first","affiliation":[{"name":"Department of Physiology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4574-8605","authenticated-orcid":false,"given":"Aurel","family":"Popa-Wagner","sequence":"additional","affiliation":[{"name":"Department of Neurology, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronica","family":"Sfredel","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4458-2298","authenticated-orcid":false,"given":"Smaranda Ioana","family":"Mitran","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7543-3084","authenticated-orcid":false,"given":"Alexandra Oltea","family":"Dan","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anca-Maria","family":"\u021auc\u0103","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra Nicoleta","family":"Preda","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor","family":"Raicea","sequence":"additional","affiliation":[{"name":"Department of Cardiology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugen","family":"\u021aieranu","sequence":"additional","affiliation":[{"name":"Department of Cardiology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7378-7787","authenticated-orcid":false,"given":"Drago\u0219","family":"Cozma","sequence":"additional","affiliation":[{"name":"Department of Cardiology, \u201cVictor Babes\u201d University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7163-8528","authenticated-orcid":false,"given":"Radu","family":"V\u0103t\u0103\u0219escu","sequence":"additional","affiliation":[{"name":"Cardio-Thoracic Pathology Department, \u201cCarol Davila\u201d University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Hinault, C., Caroli-Bosc, P., Bost, F., and Chevalier, N. (2023). Critical Overview on Endocrine Disruptors in Diabetes Mellitus. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24054537"},{"key":"ref_2","unstructured":"International Diabetes Federation (2021). IDF Diabetes Atlas, International Diabetes Federation. [10th ed.]. Available online: https:\/\/diabetesatlas.org."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"26","DOI":"10.2337\/dci23-0085","article-title":"Economic Costs of Diabetes in the U.S. in 2022","volume":"47","author":"Parker","year":"2024","journal-title":"Diabetes Care"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.hfc.2019.02.003","article-title":"Diabetic Cardiomyopathy: Definition, Diagnosis, and Therapeutic Implications","volume":"15","author":"Paolillo","year":"2019","journal-title":"Heart Fail. Clin."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/s12933-023-01747-1","article-title":"Acid sphingomyelinase promotes diabetic cardiomyopathy via NADPH oxidase 4 mediated apoptosis","volume":"22","author":"Liu","year":"2023","journal-title":"Cardiovasc. Diabetol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1161\/CIRCRESAHA.118.314665","article-title":"Diabetic Cardiomyopathy","volume":"124","author":"Dillmann","year":"2019","journal-title":"Circ. Res."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Tao, W., Wang, G., and Wei, J. (2021). The Role of Chitosan Oligosaccharide in Metabolic Syndrome: A Review of Possible Mechanisms. Mar. Drugs, 19.","DOI":"10.3390\/md19090501"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.3390\/md13031133","article-title":"Chitin and chitosan preparation from marine sources. Structure, properties and applications","volume":"13","author":"Younes","year":"2015","journal-title":"Mar. Drugs"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1016\/j.foodchem.2015.06.076","article-title":"Advances in characterisation and biological activities of chitosan and chitosan oligosaccharides","volume":"190","author":"Zou","year":"2016","journal-title":"Food Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.jconrel.2004.10.012","article-title":"Influence of molecular weight on oral absorption of water soluble chitosans","volume":"102","author":"Chae","year":"2005","journal-title":"J. Control. Release"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/j.carbpol.2007.06.016","article-title":"Absorption and distribution of chitosan in mice after oral administration","volume":"71","author":"Zeng","year":"2008","journal-title":"Carbohydr. Polym."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.foodchem.2007.08.044","article-title":"Hypocholesterolaemic effects of different chitosan samples in vitro and in vivo","volume":"107","author":"Liu","year":"2008","journal-title":"Food Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5795","DOI":"10.1021\/jf100662r","article-title":"Chitosan Reduces Gluconeogenesis and Increases Glucose Uptake in Skeletal Muscle in Streptozotocin-Induced Diabetic Rats","volume":"58","author":"Liu","year":"2010","journal-title":"J. Agric. Food Chem."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Tzeng, H.P., Liu, S.H., and Chiang, M.T. (2022). Antidiabetic Properties of Chitosan and Its Derivatives. Mar. Drugs, 20.","DOI":"10.3390\/md20120784"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Guan, Z., and Feng, Q. (2022). Chitosan and Chitooligosaccharide: The Promising Non-Plant-Derived Prebiotics with Multiple Biological Activities. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23126761"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s12933-019-0980-4","article-title":"Sodium-glucose cotransporter 2 inhibitor Dapagliflozin attenuates diabetic cardiomyopathy","volume":"19","author":"Arow","year":"2020","journal-title":"Cardiovasc. Diabetol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1177\/1479164114561992","article-title":"Probing SGLT2 as a therapeutic target for diabetes: Basic physiology and consequences","volume":"12","author":"Gallo","year":"2015","journal-title":"Diab Vasc. Dis. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1093\/eurheartj\/ehz486","article-title":"2019 ESC Guidelines on diabetes, pre-diabetes, and cardiovascular diseases developed in collaboration with the EASD","volume":"41","author":"Cosentino","year":"2020","journal-title":"Eur. Heart J."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"129","DOI":"10.4093\/dmj.2020.0285","article-title":"Application of Animal Models in Diabetic Cardiomyopathy","volume":"45","author":"Lee","year":"2021","journal-title":"Diabetes Metab. J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"404","DOI":"10.3904\/kjim.2016.208","article-title":"Diabetic cardiomyopathy: Where we are and where we are going","volume":"32","author":"Lee","year":"2017","journal-title":"Korean J. Intern. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"177","DOI":"10.4239\/wjd.v4.i5.177","article-title":"Diabetic cardiomyopathy: Pathophysiology, diagnostic evaluation and management","volume":"4","author":"Pappachan","year":"2013","journal-title":"World J. Diabetes"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1038\/nrendo.2015.216","article-title":"Insulin resistance and hyperinsulinaemia in diabetic cardiomyopathy","volume":"12","author":"Jia","year":"2016","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1038\/s41569-020-0339-2","article-title":"Mechanisms of diabetic cardiomyopathy and potential therapeutic strategies: Preclinical and clinical evidence","volume":"17","author":"Tan","year":"2020","journal-title":"Nat. Rev. Cardiol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Pan, K.L., Hsu, Y.C., Chang, S.T., Chung, C.M., and Lin, C.L. (2023). The Role of Cardiac Fibrosis in Diabetic Cardiomyopathy: From Pathophysiology to Clinical Diagnostic Tools. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24108604"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.mam.2018.07.001","article-title":"Cardiac fibrosis: Cell biological mechanisms, molecular pathways and therapeutic opportunities","volume":"65","author":"Frangogiannis","year":"2019","journal-title":"Mol. Asp. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.1093\/cvr\/cvaa324","article-title":"Cardiac fibrosis","volume":"117","author":"Frangogiannis","year":"2021","journal-title":"Cardiovasc. Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"H934","DOI":"10.1152\/ajpheart.00238.2018","article-title":"Characterization of a mouse model of obesity-related fibrotic cardiomyopathy that recapitulates features of human heart failure with preserved ejection fraction","volume":"315","author":"Alex","year":"2018","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"860","DOI":"10.4239\/wjd.v5.i6.860","article-title":"Molecular mechanisms of AGE\/RAGE-mediated fibrosis in the diabetic heart","volume":"5","author":"Zhao","year":"2014","journal-title":"World J. Diabetes"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.yjmcc.2015.12.011","article-title":"Diabetes-associated cardiac fibrosis: Cellular effectors, molecular mechanisms and therapeutic opportunities","volume":"90","author":"Russo","year":"2016","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.actbio.2020.10.045","article-title":"Efficacy of epicardial implantation of acellular chitosan hydrogels in ischemic and nonischemic heart failure: Impact of the acetylation degree of chitosan","volume":"119","author":"Ragot","year":"2021","journal-title":"Acta Biomater."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Wang, Y., Liu, Y., Gong, T., and Hou, M. (2022). The Anti-inflammatory Effect of Chitosan Oligosaccharide on Heart Failure in Mice. Biomed. Res. Int., 2022.","DOI":"10.1155\/2022\/8746530"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2528","DOI":"10.1161\/CIRCULATIONAHA.119.040130","article-title":"Effect of Dapagliflozin on Heart Failure and Mortality in Type 2 Diabetes Mellitus","volume":"139","author":"Kato","year":"2019","journal-title":"Circulation"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1056\/NEJMoa1812389","article-title":"Dapagliflozin and Cardiovascular Outcomes in Type 2 Diabetes","volume":"380","author":"Wiviott","year":"2019","journal-title":"N. Engl. J. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1995","DOI":"10.1056\/NEJMoa1911303","article-title":"Dapagliflozin in Patients with Heart Failure and Reduced Ejection Fraction","volume":"381","author":"McMurray","year":"2019","journal-title":"N. Engl. J. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1001\/jama.2020.1906","article-title":"Effect of Dapagliflozin on Worsening Heart Failure and Cardiovascular Death in Patients with Heart Failure with and without Diabetes","volume":"323","author":"Petrie","year":"2020","journal-title":"JAMA"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"907757","DOI":"10.3389\/fendo.2022.907757","article-title":"Signaling Pathways Related to Oxidative Stress in Diabetic Cardiomyopathy","volume":"13","author":"Peng","year":"2022","journal-title":"Front. Endocrinol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"H\u00f6lscher, M.E., Bode, C., and Bugger, H. (2016). Diabetic Cardiomyopathy: Does the Type of Diabetes Matter?. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17122136"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2108","DOI":"10.1093\/cvr\/cvaa256","article-title":"The effects of liraglutide and dapagliflozin on cardiac function and structure in a multi-hit mouse model of heart failure with preserved ejection fraction","volume":"117","author":"Withaar","year":"2021","journal-title":"Cardiovasc. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1093\/ajh\/hpz016","article-title":"Dapagliflozin Attenuates Cardiac Remodeling in Mice Model of Cardiac Pressure Overload","volume":"32","author":"Shi","year":"2019","journal-title":"Am. J. Hypertens."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1161\/CIRCULATIONAHA.114.014937","article-title":"A Conductive Polymer Hydrogel Supports Cell Electrical Signaling and Improves Cardiac Function After Implantation into Myocardial Infarct","volume":"132","author":"Mihic","year":"2015","journal-title":"Circulation"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Patel, B., Manne, R., Patel, D.B., Gorityala, S., Palaniappan, A., and Kurakula, M. (2021). Chitosan as Functional Biomaterial for Designing Delivery Systems in Cardiac Therapies. Gels, 7.","DOI":"10.3390\/gels7040253"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"588","DOI":"10.3892\/etm.2017.4026","article-title":"Chitosan hydrogel improves mesenchymal stem cell transplant survival and cardiac function following myocardial infarction in rats","volume":"13","author":"Xu","year":"2017","journal-title":"Exp. Ther. Med."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Nakamura, K., Miyoshi, T., Yoshida, M., Akagi, S., Saito, Y., Ejiri, K., Matsuo, N., Ichikawa, K., Iwasaki, K., and Naito, T. (2022). Pathophysiology and Treatment of Diabetic Cardiomyopathy and Heart Failure in Patients with Diabetes Mellitus. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23073587"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1016\/j.jacl.2017.01.018","article-title":"Dapagliflozin in patients with type II diabetes mellitus, with and without elevated triglyceride and reduced high-density lipoprotein cholesterol levels","volume":"11","author":"Bays","year":"2017","journal-title":"J. Clin. Lipidol."},{"key":"ref_45","first-page":"80","article-title":"Chitosan oligosaccharides inhibit adipogenesis in 3T3-L1 adipocytes","volume":"18","author":"Cho","year":"2008","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.sjbs.2016.09.008","article-title":"The effects of COST on the differentiation of 3T3-L1 preadipocytes and the mechanism of action","volume":"24","author":"Kong","year":"2017","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Tuleta, I., and Frangogiannis, N.G. (2021). Diabetic fibrosis. Biochim. Biophys. Acta Mol. Basis Dis., 1867.","DOI":"10.1016\/j.bbadis.2020.166044"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2949","DOI":"10.2337\/diacare.26.10.2949","article-title":"Diabetic cardiomyopathy","volume":"26","author":"Bell","year":"2003","journal-title":"Diabetes Care"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"479258","DOI":"10.3389\/fendo.2020.479258","article-title":"Cardioprotective Effect of Decorin in Type 2 Diabetes","volume":"11","author":"Chen","year":"2020","journal-title":"Front. Endocrinol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1161\/01.RES.86.12.1259","article-title":"Interleukin-1beta and tumor necrosis factor-alpha decrease collagen synthesis and increase matrix metalloproteinase activity in cardiac fibroblasts in vitro","volume":"86","author":"Siwik","year":"2000","journal-title":"Circ. Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1210\/en.2007-0877","article-title":"Oxidative stress triggers cardiac fibrosis in the heart of diabetic rats","volume":"149","author":"Aragno","year":"2008","journal-title":"Endocrinology"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"165","DOI":"10.2174\/1871529X11313020010","article-title":"Oxidative stress and transforming growth factor-\u03b21-induced cardiac fibrosis","volume":"13","author":"Purnomo","year":"2013","journal-title":"Cardiovasc. Hematol. Disord. Drug Targets"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1161\/CIRCRESAHA.110.217117","article-title":"Activation of protein kinase C isoforms and its impact on diabetic complications","volume":"106","author":"Geraldes","year":"2010","journal-title":"Circ. Res."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Crisafulli, A., Pagliaro, P., Roberto, S., Cugusi, L., Mercuro, G., Lazou, A., Beauloye, C., Bertrand, L., Hausenloy, D.J., and Aragno, M. (2020). Diabetic Cardiomyopathy and Ischemic Heart Disease: Prevention and Therapy by Exercise and Conditioning. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21082896"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"641","DOI":"10.2337\/db06-1163","article-title":"Contributions of inflammation and cardiac matrix metalloproteinase activity to cardiac failure in diabetic cardiomyopathy: The role of angiotensin type 1 receptor antagonism","volume":"56","author":"Westermann","year":"2007","journal-title":"Diabetes"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Evangelista, I., Nuti, R., Picchioni, T., Dotta, F., and Palazzuoli, A. (2019). Molecular Dysfunction and Phenotypic Derangement in Diabetic Cardiomyopathy. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20133264"},{"key":"ref_57","unstructured":"National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals (2011). Guide for the Care and Use of Laboratory Animals, National Academies Press (US). [8th ed.]."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"521","DOI":"10.47162\/RJME.61.2.22","article-title":"Alpha-lipoic acid and vitamin B complex slow down the changes in mice diabetic cardiomyopathy","volume":"61","author":"Florescu","year":"2020","journal-title":"Rom. J. Morphol. Embryol."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Donoiu, I., T\u00e2rtea, G., Sfredel, V., Raicea, V., \u021auc\u0103, A.M., Preda, A.N., Cozma, D., and V\u0103t\u0103\u0219escu, R. (2023). Dapagliflozin Ameliorates Neural Damage in the Heart and Kidney of Diabetic Mice. Biomedicines, 11.","DOI":"10.3390\/biomedicines11123324"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"S19","DOI":"10.2337\/dc23-S002","article-title":"2. Classification and Diagnosis of Diabetes: Standards of Care in Diabetes-2023","volume":"46","author":"ElSayed","year":"2023","journal-title":"Diabetes Care"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"3081397","DOI":"10.1155\/2022\/3081397","article-title":"Antioxidative Stress and Antiapoptosis Effect of Chitosan Nanoparticles to Protect Cardiac Cell Damage on Streptozotocin-Induced Diabetic Rat","volume":"2022","author":"Wardani","year":"2022","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.echo.2014.10.003","article-title":"Recommendations for cardiac chamber quantification by echocardiography in adults: An update from the American Society of Echocardiography and the European Association of Cardiovascular Imaging","volume":"28","author":"Lang","year":"2015","journal-title":"J. Am. Soc. Echocardiogr."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/4\/2118\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:58:00Z","timestamp":1760104680000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/4\/2118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,9]]},"references-count":62,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2024,2]]}},"alternative-id":["ijms25042118"],"URL":"https:\/\/doi.org\/10.3390\/ijms25042118","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,9]]}}}