{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T07:33:45Z","timestamp":1785310425915,"version":"3.55.0"},"reference-count":57,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,2,14]],"date-time":"2024-02-14T00:00:00Z","timestamp":1707868800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"New Frontiers in Research Fund","award":["NFRFE-2019-00332"],"award-info":[{"award-number":["NFRFE-2019-00332"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Calcific aortic valve disease (CAVD) is characterized by the fibrosis and mineralization of the aortic valve, which leads to aortic stenosis and heart failure. At the cellular level, this is due to the osteoblastic-like differentiation of valve interstitial cells (VICs), resulting in the calcification of the tissue. Unfortunately, human VICs are not readily available to study CAVD pathogenesis and the implicated mechanisms in vitro; however, adipose-derived stromal\/stem cells (ASCs), carrying the patient\u2019s specific genomic features, have emerged as a promising cell source to model cardiovascular diseases due to their multipotent nature, availability, and patient-specific characteristics. In this study, we describe a comprehensive transcriptomic analysis of tissue-engineered, scaffold-free, ASC-embedded mineralized tissue sheets using bulk RNA sequencing. Bioinformatic and gene set enrichment analyses revealed the up-regulation of genes associated with the organization of the extracellular matrix (ECM), suggesting that the ECM could play a vital role in the enhanced mineralization observed in these tissue-engineered ASC-embedded sheets. Upon comparison with publicly available gene expression datasets from CAVD patients, striking similarities emerged regarding cardiovascular diseases and ECM functions, suggesting a potential link between ECM gene expression and CAVDs pathogenesis. A matrisome-related sub-analysis revealed the ECM microenvironment promotes the transcriptional activation of the master gene runt-related transcription factor 2 (RUNX2), which is essential in CAVD development. Tissue-engineered ASC-embedded sheets with enhanced mineralization could be a valuable tool for research and a promising avenue for the identification of more effective aortic valve replacement therapies.<\/jats:p>","DOI":"10.3390\/ijms25042291","type":"journal-article","created":{"date-parts":[[2024,2,14]],"date-time":"2024-02-14T11:18:46Z","timestamp":1707909526000},"page":"2291","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Transcriptomic Analysis of Mineralized Adipose-Derived Stem Cell Tissues for Calcific Valve Disease Modelling"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7815-1795","authenticated-orcid":false,"given":"Alyssa","family":"Brodeur","sequence":"first","affiliation":[{"name":"Department of Surgery, Faculty of Medicine, Universit\u00e9 Laval, Quebec City, QC G1V 5C3, Canada"},{"name":"Division of Regenerative Medicine, CHU de Quebec Universit\u00e9 Laval Research Centre, Quebec City, QC G1J 5B3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Roy","sequence":"additional","affiliation":[{"name":"Department of Surgery, Faculty of Medicine, Universit\u00e9 Laval, Quebec City, QC G1V 5C3, Canada"},{"name":"Division of Regenerative Medicine, CHU de Quebec Universit\u00e9 Laval Research Centre, Quebec City, QC G1J 5B3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydia","family":"Touzel-Desch\u00eanes","sequence":"additional","affiliation":[{"name":"Department of Surgery, Faculty of Medicine, Universit\u00e9 Laval, Quebec City, QC G1V 5C3, Canada"},{"name":"Division of Regenerative Medicine, CHU de Quebec Universit\u00e9 Laval Research Centre, Quebec City, QC G1J 5B3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Bianco","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, CHU de Quebec Universit\u00e9 Laval Research Centre, Quebec City, QC G1V 5C3, Canada"},{"name":"Computational Biology Laboratory, CHU de Quebec Universit\u00e9 Laval Research Centre, Quebec City, QC G1V 4G2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaud","family":"Droit","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, CHU de Quebec Universit\u00e9 Laval Research Centre, Quebec City, QC G1V 5C3, Canada"},{"name":"Computational Biology Laboratory, CHU de Quebec Universit\u00e9 Laval Research Centre, Quebec City, QC G1V 4G2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Fradette","sequence":"additional","affiliation":[{"name":"Department of Surgery, Faculty of Medicine, Universit\u00e9 Laval, Quebec City, QC G1V 5C3, Canada"},{"name":"Division of Regenerative Medicine, CHU de Quebec Universit\u00e9 Laval Research Centre, Quebec City, QC G1J 5B3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6936-4943","authenticated-orcid":false,"given":"Jean","family":"Ruel","sequence":"additional","affiliation":[{"name":"Division of Regenerative Medicine, CHU de Quebec Universit\u00e9 Laval Research Centre, Quebec City, QC G1J 5B3, Canada"},{"name":"Department of Mechanical Engineering, Faculty of Sciences and Engineering, Laval University, Quebec City, QC G1V 0A6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3825-4885","authenticated-orcid":false,"given":"Fran\u00e7ois","family":"Gros-Louis","sequence":"additional","affiliation":[{"name":"Department of Surgery, Faculty of Medicine, Universit\u00e9 Laval, Quebec City, QC G1V 5C3, Canada"},{"name":"Division of Regenerative Medicine, CHU de Quebec Universit\u00e9 Laval Research Centre, Quebec City, QC G1J 5B3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1093\/eurheartj\/ehab757","article-title":"Calcific aortic valve disease: From molecular and cellular mechanisms to medical therapy","volume":"43","author":"Kraler","year":"2022","journal-title":"Eur. 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