{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:10:33Z","timestamp":1787962233255,"version":"build-2784847793"},"reference-count":43,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,2,16]],"date-time":"2024-02-16T00:00:00Z","timestamp":1708041600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Wroc\u0142aw University of Environmental and Life Sciences"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Human adenovirus 36 (HAdV-D36) is presently the sole virus identified to be associated with an elevated risk of obesity in both humans and animals. However, its impact on embryonated chicken eggs (ECEs) remains unexplored. This study endeavoured to examine the influence of HAdV-D36 on embryonic development by utilizing embryonated chicken eggs as a dynamic model. To simulate various infection routes, the allantoic cavity and the yolk sac of ECEs were inoculated with HAdV-D36. Subsequently, embryos from both the experimental (inoculated with virus) and control (inoculated with PBS) groups were weighed and subjected to daily histological examination. The daily embryo weights were assessed and compared between groups using the Shapiro\u2013Wilk test. Histopathological changes in tissues were examined and compared between the tested and control groups to ascertain physiological alterations induced by the virus. Our study confirmed a significant increase in the body weight of ECEs. However, this phenomenon was not attributable to adipose tissue development; rather, it was characterized by an augmented number of cells in all observed tissues compared to control subjects. We posit that HAdV-D36 may impact developing organisms through mechanisms other than enhanced adipose tissue development. Specifically, our findings indicate an increased number of cells in all tissues, a phenomenon that occurs through an as-yet-unexplored pathway.<\/jats:p>","DOI":"10.3390\/ijms25042341","type":"journal-article","created":{"date-parts":[[2024,2,16]],"date-time":"2024-02-16T06:00:25Z","timestamp":1708063225000},"page":"2341","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Impact of Human Adenovirus 36 on Embryonated Chicken Eggs: Insights into Growth Mechanisms"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5245-1733","authenticated-orcid":false,"given":"Aleksandra","family":"Pogorzelska","sequence":"first","affiliation":[{"name":"Department of Pathology, Division of Microbiology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 31 C. K. Norwida Street, 50-573 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0682-4743","authenticated-orcid":false,"given":"Piotr","family":"Kuropka","sequence":"additional","affiliation":[{"name":"Department of Biostructure and Animal Physiology, Division of Histology and Embryology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, C. K. Norwida 25, 50-635 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8509-7507","authenticated-orcid":false,"given":"Dominika","family":"Stygar","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medical Science in Zabrze, Medical University of Silesia, 19 Jordana Street, 40-808 Zabrze, Poland"},{"name":"SLU University Animal Hospital, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1809-9531","authenticated-orcid":false,"given":"Katarzyna","family":"Michalczyk","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medical Science in Zabrze, Medical University of Silesia, 19 Jordana Street, 40-808 Zabrze, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0111-438X","authenticated-orcid":false,"given":"El\u017cbieta","family":"Che\u0142mecka","sequence":"additional","affiliation":[{"name":"Department of Medical Statistic, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, 30 Ostrog\u00f3rska Street, 40-200 Sosnowiec, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3879-6548","authenticated-orcid":false,"given":"Jolanta","family":"Zalejska-Fiolka","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 19 Jordana Street, 41-808 Zabrze, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agata","family":"Kublicka","sequence":"additional","affiliation":[{"name":"Department of Pathology, Division of Microbiology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 31 C. K. Norwida Street, 50-573 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6953-9926","authenticated-orcid":false,"given":"Paulina","family":"Janicka","sequence":"additional","affiliation":[{"name":"Department of Pathology, Division of Microbiology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 31 C. K. Norwida Street, 50-573 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6694-2872","authenticated-orcid":false,"given":"Barbara","family":"Ba\u017can\u00f3w","sequence":"additional","affiliation":[{"name":"Department of Pathology, Division of Microbiology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 31 C. K. Norwida Street, 50-573 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.1467-789X.2009.00677.x","article-title":"Viral obesity: Fact or fiction?","volume":"11","author":"Mitra","year":"2010","journal-title":"Obes. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S1043-4526(06)52002-9","article-title":"Infectobesity: Obesity of Infectious Origin","volume":"52","author":"Pasarica","year":"2007","journal-title":"Adv. Food Nutr. 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