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We previously showed that chronic binge alcohol (CBA) administration, with or without simian immunodeficiency virus (SIV), decreases differentiation capacity of male rhesus macaque myoblasts. We hypothesized that short-term alcohol and CBA\/SIV would synergistically decrease differentiation capacity and impair bioenergetic parameters in female macaque myoblasts. Myoblasts from na\u00efve (CBA\u2212\/SIV\u2212), vehicle [VEH]\/SIV, and CBA\/SIV (N = 4\u20136\/group) groups were proliferated (3 days) and differentiated (5 days) with 0 or 50 mM ethanol (short-term). CBA\/SIV decreased differentiation and increased non-mitochondrial oxygen consumption rate (OCR) versus na\u00efve and\/or VEH\/SIV. Short-term alcohol decreased differentiation; increased maximal and non-mitochondrial OCR, mitochondrial reactive oxygen species (ROS) production, and aldolase activity; and decreased glycolytic measures, ATP production, mitochondrial membrane potential (\u0394\u03a8m), and pyruvate kinase activity. Mitochondrial ROS production was closely associated with mitochondrial network volume, and differentiation indices were closely associated with key bioenergetic health and function parameters. Results indicate that short-term alcohol and CBA non-synergistically decrease myoblast differentiation capacity. Short-term alcohol impaired myoblast glycolytic function, driving the bioenergetic deficit. Results suggest potentially differing mechanisms underlying decreased differentiation capacity with short-term alcohol and CBA, highlighting the need to elucidate the impact of different alcohol use patterns on myopathy.<\/jats:p>","DOI":"10.3390\/ijms25042448","type":"journal-article","created":{"date-parts":[[2024,2,20]],"date-time":"2024-02-20T04:04:14Z","timestamp":1708401854000},"page":"2448","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Alcohol Impairs Bioenergetics and Differentiation Capacity of Myoblasts from Simian Immunodeficiency Virus-Infected Female Macaques"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1865-1971","authenticated-orcid":false,"given":"Danielle E.","family":"Levitt","sequence":"first","affiliation":[{"name":"Department of Physiology, School of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA"},{"name":"Comprehensive Alcohol-HIV\/AIDS Research Center, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA"},{"name":"Department of Kinesiology and Sport Management, Texas Tech University, Lubbock, TX 79409, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brianna L.","family":"Bourgeois","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA"},{"name":"Comprehensive Alcohol-HIV\/AIDS Research Center, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keishla M.","family":"Rodr\u00edguez-Graciani","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA"},{"name":"Comprehensive Alcohol-HIV\/AIDS Research Center, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3598-384X","authenticated-orcid":false,"given":"Patricia E.","family":"Molina","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA"},{"name":"Comprehensive Alcohol-HIV\/AIDS Research Center, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liz","family":"Simon","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA"},{"name":"Comprehensive Alcohol-HIV\/AIDS Research Center, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,19]]},"reference":[{"key":"ref_1","unstructured":"SAMHSA, Center for Behavioral Health Statistics and Quality (2021). 2021 National Survey on Drug Use and Health, Table 2.28B\u2014Binge Alcohol Use in Past Month: Among People Aged 12 or Older; by Age Group and Demographic Characteristics, Percentages, SAMHSA."},{"key":"ref_2","unstructured":"SAMHSA, Center for Behavioral Health Statistics and Quality (2021). 2021 National Survey on Drug Use and Health, Table 2.29B\u2014Heavy Alcohol Use in Past Month: Among People Aged 12 or Older; by Age Group and Demographic Characteristics, Percentages, SAMHSA."},{"key":"ref_3","unstructured":"SAMHSA, Center for Behavioral Health Statistics and Quality (2021). 2021 National Survey on Drug Use and Health, Table 5.6A\u2014Alcohol Use Disorder in Past Year: Among People Aged 12 or Older; by Age Group and Demographic Characteristics, Numbers in Thousands, SAMHSA."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1186\/s13011-019-0240-3","article-title":"The Prevalence of Alcohol Use Disorders among People Living with HIV\/AIDS: A Systematic Review and Meta-Analysis","volume":"14","author":"Duko","year":"2019","journal-title":"Subst. 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