{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T17:14:02Z","timestamp":1773594842993,"version":"3.50.1"},"reference-count":51,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,2,24]],"date-time":"2024-02-24T00:00:00Z","timestamp":1708732800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Danish Heart Foundation","award":["R22-A1187-B615"],"award-info":[{"award-number":["R22-A1187-B615"]}]},{"name":"Odense University Hospital Research Fund","award":["R22-A1187-B615"],"award-info":[{"award-number":["R22-A1187-B615"]}]},{"name":"Lundbeck Foundation","award":["R22-A1187-B615"],"award-info":[{"award-number":["R22-A1187-B615"]}]},{"name":"National Program for Research Infrastructure 2007 from the Danish Agency for Science, Technology, and Innovation","award":["R22-A1187-B615"],"award-info":[{"award-number":["R22-A1187-B615"]}]},{"name":"Fabrikant Vilhelm Pedersen og Hustrus Legat on recommendation by the Novo Nordisk Foundation","award":["R22-A1187-B615"],"award-info":[{"award-number":["R22-A1187-B615"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Acute myocardial infarction (AMI) is a major cause of mortality and morbidity worldwide, yet biomarkers for AMI in the short- or medium-term are lacking. We apply the discordant twin pair design, reducing genetic and environmental confounding, by linking nationwide registry data on AMI diagnoses to a survey of 12,349 twins, thereby identifying 39 twin pairs (48\u201379 years) discordant for their first-ever AMI within three years after blood sampling. Mass spectrometry of blood plasma identified 715 proteins. Among 363 proteins with a call rate &gt; 50%, imputation and stratified Cox regression analysis revealed seven significant proteins (FDR &lt; 0.05): FGD6, MCAM, and PIK3CB reflected an increased level in AMI twins relative to their non-AMI co-twins (HR &gt; 1), while LBP, IGHV3-15, C1RL, and APOC4 reflected a decreased level in AMI twins relative to their non-AMI co-twins (HR &lt; 1). Additional 50 proteins were nominally significant (p &lt; 0.05), and bioinformatics analyses of all 57 proteins revealed biology within hemostasis, coagulation cascades, the immune system, and the extracellular matrix. A protein\u2013protein-interaction network revealed Fibronectin 1 as a central hub. Finally, technical validation confirmed MCAM, LBP, C1RL, and APOC3. We put forward novel biomarkers for incident AMI, a part of the proteome field where markers are surprisingly rare and where additional studies are highly needed.<\/jats:p>","DOI":"10.3390\/ijms25052638","type":"journal-article","created":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T06:50:23Z","timestamp":1708930223000},"page":"2638","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Mass Spectrometry-Based Proteome Study of Twin Pairs Discordant for Incident Acute Myocardial Infarction within Three Years after Blood Sampling Suggests Novel Biomarkers"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7763-3637","authenticated-orcid":false,"given":"Hans Christian","family":"Beck","sequence":"first","affiliation":[{"name":"Center for Individualized Medicine in Arterial Diseases, Department of Clinical Biochemistry, Odense University Hospital, J. B. Winsloews Vej 4, 5000 Odense, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asmus Cosmos","family":"Skovgaard","sequence":"additional","affiliation":[{"name":"The Danish Twin Registry and Epidemiology, Biostatistics and Biodemography, Department of Public Health, University of Southern Denmark, Campusvej 55, 5230 Odense, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4184-7518","authenticated-orcid":false,"given":"Afsaneh","family":"Mohammadnejad","sequence":"additional","affiliation":[{"name":"The Danish Twin Registry and Epidemiology, Biostatistics and Biodemography, Department of Public Health, University of Southern Denmark, Campusvej 55, 5230 Odense, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1480-6550","authenticated-orcid":false,"given":"Nicolai Bj\u00f8dstrup","family":"Palstr\u00f8m","sequence":"additional","affiliation":[{"name":"Center for Individualized Medicine in Arterial Diseases, Department of Clinical Biochemistry, Odense University Hospital, J. B. Winsloews Vej 4, 5000 Odense, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Palle Fruekilde","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Center for Individualized Medicine in Arterial Diseases, Department of Clinical Biochemistry, Odense University Hospital, J. B. Winsloews Vej 4, 5000 Odense, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1573-8908","authenticated-orcid":false,"given":"Jonas","family":"Mengel-From","sequence":"additional","affiliation":[{"name":"The Danish Twin Registry and Epidemiology, Biostatistics and Biodemography, Department of Public Health, University of Southern Denmark, Campusvej 55, 5230 Odense, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9630-9149","authenticated-orcid":false,"given":"Jacob","family":"Hjelmborg","sequence":"additional","affiliation":[{"name":"The Danish Twin Registry and Epidemiology, Biostatistics and Biodemography, Department of Public Health, University of Southern Denmark, Campusvej 55, 5230 Odense, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars Melholt","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"Center for Individualized Medicine in Arterial Diseases, Department of Clinical Biochemistry, Odense University Hospital, J. B. Winsloews Vej 4, 5000 Odense, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5268-3366","authenticated-orcid":false,"given":"Mette","family":"Soerensen","sequence":"additional","affiliation":[{"name":"The Danish Twin Registry and Epidemiology, Biostatistics and Biodemography, Department of Public Health, University of Southern Denmark, Campusvej 55, 5230 Odense, Denmark"},{"name":"Department of Clinical Genetics, Odense University Hospital, J. B. Winsloews Vej 4, 5000 Odense, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,24]]},"reference":[{"key":"ref_1","unstructured":"(2022, December 16). Global Burden of Disease 2019. 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