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Intracerebroventricular (ICV) injection of low to moderate doses of streptozotocin (STZ) in adult male Wistar rats can reproduce classical physiopathological hallmarks of AD. This biological model is known as ICV-STZ. Most studies are focused on the description of behavioral and morphological aspects of the ICV-STZ model. However, knowledge regarding the molecular aspects of the ICV-STZ model is still incipient. Therefore, this work is a first attempt to provide a wide proteome description of the ICV-STZ model based on mass spectrometry (MS). To achieve that, samples from the pre-frontal cortex (PFC) and hippocampus (HPC) of the ICV-STZ model and control (wild-type) were used. Differential protein abundance, pathway, and network analysis were performed based on the protein identification and quantification of the samples. Our analysis revealed dysregulated biological pathways implicated in the early stages of late-onset Alzheimer\u2019s disease (LOAD), based on differentially abundant proteins (DAPs). Some of these DAPs had their mRNA expression further investigated through qRT-PCR. Our results shed light on the AD onset and demonstrate the ICV-STZ as a valid model for LOAD proteome description.<\/jats:p>","DOI":"10.3390\/ijms25052772","type":"journal-article","created":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T03:37:57Z","timestamp":1709091477000},"page":"2772","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Proteomic Analysis of a Rat Streptozotocin Model Shows Dysregulated Biological Pathways Implicated in Alzheimer\u2019s Disease"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9084-938X","authenticated-orcid":false,"given":"Esdras Matheus Gomes","family":"da Silva","sequence":"first","affiliation":[{"name":"Instituto Carlos Chagas, FIOCRUZ, Curitiba 81310-020, PR, Brazil"},{"name":"Laboratory of Toxinology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-361, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana S. G.","family":"Fischer","sequence":"additional","affiliation":[{"name":"Instituto Carlos Chagas, FIOCRUZ, Curitiba 81310-020, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isadora de Lourdes Signorini","family":"Souza","sequence":"additional","affiliation":[{"name":"Laboratory of Polymorphisms and Linkage, Federal University of Paran\u00e1, Curitiba 81530-001, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda Caroline Camillo","family":"Andrade","sequence":"additional","affiliation":[{"name":"Instituto Carlos Chagas, FIOCRUZ, Curitiba 81310-020, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9687-9838","authenticated-orcid":false,"given":"Leonardo de Castro e","family":"Souza","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Federal University of Paran\u00e1, Curitiba 80050-540, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Kaoann de","family":"Andrade","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Federal University of Paran\u00e1, Curitiba 80050-540, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6530-3350","authenticated-orcid":false,"given":"Paulo C.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Instituto Carlos Chagas, FIOCRUZ, Curitiba 81310-020, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2591-8857","authenticated-orcid":false,"given":"Ricardo Lehtonen Rodrigues","family":"Souza","sequence":"additional","affiliation":[{"name":"Laboratory of Polymorphisms and Linkage, Federal University of Paran\u00e1, Curitiba 81530-001, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Aparecida Barbato Frazao","family":"Vital","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Federal University of Paran\u00e1, Curitiba 80050-540, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5672-7848","authenticated-orcid":false,"given":"Fabio","family":"Passetti","sequence":"additional","affiliation":[{"name":"Instituto Carlos Chagas, FIOCRUZ, Curitiba 81310-020, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1002\/ca.980080612","article-title":"An English Translation of Alzheimer\u2019s 1907 Paper, \u201cUber Eine Eigenartige Erkankung Der Hirnrinde\u201d","volume":"8","author":"Stelzmann","year":"1995","journal-title":"Clin. 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