{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T20:40:43Z","timestamp":1779396043877,"version":"3.53.1"},"reference-count":105,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,3,9]],"date-time":"2024-03-09T00:00:00Z","timestamp":1709942400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Establishment Labs","award":["D152500-015-015"],"award-info":[{"award-number":["D152500-015-015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Breast cancer is the most common cancer in women globally, often necessitating mastectomy and subsequent breast reconstruction. Silicone mammary implants (SMIs) play a pivotal role in breast reconstruction, yet their interaction with the host immune system and microbiome remains poorly understood. This study investigates the impact of SMI surface topography on host antimicrobial responses, wound proteome dynamics, and microbial colonization. Biological samples were collected from ten human patients undergoing breast reconstruction with SMIs. Mass spectrometry profiles were analyzed for acute and chronic wound proteomes, revealing a nuanced interplay between topography and antimicrobial response proteins. 16S rRNA sequencing assessed microbiome dynamics, unveiling topography-specific variations in microbial composition. Surface topography alterations influenced wound proteome composition. Microbiome analysis revealed heightened diversity around rougher SMIs, emphasizing topography-dependent microbial invasion. In vitro experiments confirmed staphylococcal adhesion, growth, and biofilm formation on SMI surfaces, with increased texture correlating positively with bacterial colonization. This comprehensive investigation highlights the intricate interplay between SMI topography, wound proteome dynamics, and microbial transmission. The findings contribute to understanding host\u2013microbe interactions on SMI surfaces, essential for optimizing clinical applications and minimizing complications in breast reconstruction.<\/jats:p>","DOI":"10.3390\/ijms25063163","type":"journal-article","created":{"date-parts":[[2024,3,11]],"date-time":"2024-03-11T06:05:30Z","timestamp":1710137130000},"page":"3163","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Surface Topography, Microbial Adhesion, and Immune Responses in Silicone Mammary Implant-Associated Capsular Fibrosis"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7988-722X","authenticated-orcid":false,"given":"Ines","family":"Schoberleitner","sequence":"first","affiliation":[{"name":"Department of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Innsbruck, 6020 Innsbruck, 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Surg. J."},{"key":"ref_2","first-page":"478","article-title":"The history and development of breast implants","volume":"102","author":"Perry","year":"2020","journal-title":"Ind. Mark. Manag."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"52","DOI":"10.12998\/wjcc.v3.i1.52","article-title":"Implant biomaterials: A comprehensive review","volume":"3","author":"Saini","year":"2015","journal-title":"World J. Clin. Cases WJCC"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Mempin, M., Hu, H., Chowdhury, D., Deva, A., and Vickery, K. (2018). The A, B and C\u2019s of silicone breast implants: Anaplastic large cell lymphoma, biofilm and capsular contracture. Materials, 11.","DOI":"10.3390\/ma11122393"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.jaut.2015.08.009","article-title":"Silicone implants and lymphoma: The role of inflammation","volume":"65","author":"Bizjak","year":"2015","journal-title":"J. Autoimmun."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.it.2009.12.001","article-title":"The immunology of fibrosis: Innate and adaptive responses","volume":"31","author":"Wick","year":"2010","journal-title":"Trends Immunol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1146\/annurev-immunol-032712-095937","article-title":"The immunology of fibrosis","volume":"31","author":"Wick","year":"2013","journal-title":"Annu. Rev. Immunol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.bjps.2017.12.002","article-title":"The aetiopathogenesis of capsular contracture: A systematic review of the literature","volume":"71","author":"Bachour","year":"2018","journal-title":"J. Plast. Reconstr. Aesthetic Surg."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1097\/01.prs.0000264398.85652.9a","article-title":"Clinical and morphological conditions in capsular contracture formed around silicone breast implants","volume":"120","author":"Prantl","year":"2007","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1055\/s-0041-1731793","article-title":"Healing, Inflammation, and Fibrosis: Current Concepts in Capsular Contracture: Pathophysiology, Prevention, and Management","volume":"35","author":"Safran","year":"2021","journal-title":"Semin. Plast. Surg."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1016\/S0142-9612(02)00429-5","article-title":"Histological analysis of silicone breast implant capsules and correlation with capsular contracture","volume":"24","author":"Siggelkow","year":"2003","journal-title":"Biomaterials"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1177\/039139880402701214","article-title":"In vitro analysis of modified surfaces of silicone breast implants","volume":"27","author":"Siggelkow","year":"2004","journal-title":"Int. J. Artif. Organs"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1097\/00006534-199512000-00003","article-title":"The fate of breast implants: A critical analysis of complications and outcomes","volume":"96","author":"Handel","year":"1995","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1002\/path.2277","article-title":"Cellular and molecular mechanisms of fibrosis","volume":"214","author":"Wynn","year":"2008","journal-title":"J. Pathol."},{"key":"ref_15","first-page":"161","article-title":"Roxatidine inhibits fibrosis by inhibiting NF \u03baB and MAPK signaling in macrophages sensing breast implant surface materials","volume":"21","author":"Ji","year":"2020","journal-title":"Mol. Med. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1097\/01.GOX.0000558388.69103.8d","article-title":"Abstract 114. Targeting Wnt Signaling to Reduce Capsular Fibrosis","volume":"7","author":"Kuehlmann","year":"2019","journal-title":"Plast. Reconstr. Surg. Glob. Open"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"10269","DOI":"10.1038\/s41598-019-46439-7","article-title":"Hypoxia-induced epithelial-mesenchymal transition and fibrosis for the development of breast capsular contracture","volume":"9","author":"Kuo","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1038\/nrneph.2016.48","article-title":"TGF-\u03b2: The master regulator of fibrosis","volume":"12","author":"Meng","year":"2016","journal-title":"Nat. Rev. Nephrol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1002\/jbm.b.33954","article-title":"Characterization of serum proteins attached to distinct sol\u2013gel hybrid surfaces","volume":"106","author":"Gurruchaga","year":"2018","journal-title":"J. Biomed. Mater. Res. Part B Appl. Biomater."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.jim.2007.08.016","article-title":"Simultaneous analysis of multiple serum proteins adhering to the surface of medical grade polydimethylsiloxane elastomers","volume":"328","author":"Backovic","year":"2007","journal-title":"J. Immunol. Methods"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Schoberleitner, I., Faserl, K., Sarg, B., Egle, D., Brunner, C., and Wolfram, D. (2023). Quantitative Proteomic Characterization of Foreign Body Response towards Silicone Breast Implants Identifies Chronological Disease-Relevant Biomarker Dynamics. Biomolecules, 13.","DOI":"10.3390\/biom13020305"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1021\/pr0603755","article-title":"Identification and dynamics of proteins adhering to the surface of medical silicones in vivo and in vitro","volume":"6","author":"Backovic","year":"2006","journal-title":"J. Proteome Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1038\/s41551-021-00739-4","article-title":"The surface topography of silicone breast implants mediates the foreign body response in mice, rabbits and humans","volume":"5","author":"Doloff","year":"2021","journal-title":"Nat. Biomed. Eng."},{"key":"ref_24","unstructured":"Non-Active Surgical Implants\u2014Mammary Implants\u2014Particular Requirements (Standard No. ISO 14607:2018). Available online: https:\/\/www.iso.org\/standard\/63973.html."},{"key":"ref_25","first-page":"e3449","article-title":"Breast Implant Utilization Trends in USA versus Europe and the Impact of BIA-ALCL Publications","volume":"9","author":"Jalalabadi","year":"2021","journal-title":"Plast. Reconstr. Surg.-Glob. Open"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1097\/00006534-199708000-00065","article-title":"Anaplastic t-cell lymphoma in proximity to a saline-filled breast implant","volume":"100","author":"Keech","year":"1997","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"E245","DOI":"10.1097\/SLA.0000000000004035","article-title":"Breast Implant-associated Anaplastic Large Cell Lymphoma: A Prospective Series of 52 Patients","volume":"275","author":"Tevis","year":"2022","journal-title":"Ann. Surg."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2182","DOI":"10.1097\/01.prs.0000218184.47372.d5","article-title":"Textured surface breast implants in the prevention of capsular contracture among breast augmentation patients: A meta-analysis of randomized controlled trials","volume":"117","author":"Barnsley","year":"2006","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Cappellano, G., Ploner, C., Lobenwein, S., Sopper, S., Hoertnagl, P., Mayerl, C., Wick, N., Pierer, G., Wick, G., and Wolfram, D. (2018). Immunophenotypic characterization of human T cells after in vitro exposure to different silicone breast implant surfaces. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0192108"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Schoberleitner, I., Faserl, K., Tripp, C.H., Pechriggl, E.J., Sigl, S., Brunner, A., Hermann-Kleiter, N., Baier, L., Steinkellner, T., and Sarg, B. (2024). Silicone implant surface microtopography modulates inflammation and tissue repair in capsular fibrosis. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1342895"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Schoberleitner, I., Augustin, A., Egle, D., Brunner, C., Amort, B., Zelger, B., Brunner, A., and Wolfram, D. (2023). Is It All about Surface Topography? An Intra-Individual Clinical Outcome Analysis of Two Different Implant Surfaces in Breast Reconstruction. J. Clin. Med., 12.","DOI":"10.3390\/jcm12041315"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Mohapatra, S. (2017). Sterilization and Disinfection. Essent. Neuroanesthesia, 929, Available online: https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7158362\/.","DOI":"10.1016\/B978-0-12-805299-0.00059-2"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1086\/657579","article-title":"Disinfection and Sterilization of Prion-Contaminated Medical Instruments","volume":"31","author":"Belay","year":"2010","journal-title":"Infect. Control Hosp. Epidemiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1086\/650197","article-title":"Guideline for Disinfection and Sterilization of Prion-Contaminated Medical Instruments","volume":"31","author":"Rutala","year":"2010","journal-title":"Infect. Control Hosp. Epidemiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.2147\/TCRM.S280042","article-title":"Bacterial Biofilm Components Induce an Enhanced Inflammatory Response Against Metal Wear Particles","volume":"ume 16","author":"Dapunt","year":"2020","journal-title":"Ther. Clin. Risk Manag."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"309","DOI":"10.20452\/pamw.1780","article-title":"Antibiotic resistance: A \u201cdark side\u201d of biofilm-associated chronic infections","volume":"123","author":"Marcinkiewicz","year":"2013","journal-title":"Pol. Arch. Med. Wewn."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1093\/asj\/sjy216","article-title":"Back to Basics: Could the Preoperative Skin Antiseptic Agent Help Prevent Biofilm-Related Capsular Contracture?","volume":"39","author":"Carvajal","year":"2019","journal-title":"Aesthetic Surg. J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"881","DOI":"10.3201\/eid0809.020063","article-title":"Biofilms: Microbial Life on Surfaces","volume":"8","author":"Donlan","year":"2002","journal-title":"Emerg. Infect. Dis."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.1126\/science.284.5418.1318","article-title":"Bacterial biofilms: A common cause of persistent infections","volume":"284","author":"Costerton","year":"1999","journal-title":"Science"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1002\/bjs.9084","article-title":"Bacterial biofilms and capsular contracture in patients with breast implants","volume":"100","author":"Rieger","year":"2013","journal-title":"Br. J. Surg."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1093\/asj\/sjv177","article-title":"The relationship of bacterial biofilms and capsular contracture in breast implants","volume":"36","author":"Ajdic","year":"2016","journal-title":"Aesthetic Surg. J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"55","DOI":"10.4248\/IJOS11026","article-title":"The clinical impact of bacterial biofilms","volume":"3","author":"Ciofu","year":"2011","journal-title":"Int. J. Oral Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1097\/PRS.0b013e3182a3c105","article-title":"The role of bacterial biofilms in device-associated infection","volume":"132","author":"Deva","year":"2013","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/978-3-319-09782-4_5","article-title":"Role of biofilms in breast implant associated infections and capsular contracture","volume":"831","author":"Auba","year":"2015","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1097\/01.PRS.0000054768.14922.44","article-title":"Detection of subclinical infection in significant breast implant capsules","volume":"111","author":"Pajkos","year":"2003","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1097\/PRS.0000000000003575","article-title":"Macrotextured breast implants with defined steps to minimize bacterial contamination around the device: Experience in 42,000 implants","volume":"140","author":"Adams","year":"2017","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.bjps.2023.06.062","article-title":"Comparing the efficacy of antimicrobial pocket-irrigation protocols in an in vivo breast implant infection model","volume":"85","author":"Fliss","year":"2023","journal-title":"J. Plast. Reconstr. Aesthetic Surg."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Fern\u00e1ndez-Ibarburu, B., D\u00edaz-Navarro, M., Ibarra, G., Rivera, A., Hafian, R., Irigoyen, \u00c3, Carrillo, R., P\u00e9rez-Cano, R., Mu\u00f1oz, P., and Garc\u00eda-Ruano, \u00c1. (2022). Efficacy of Povidone Iodine Against Microbial Biofilms in Breast Implants With Different Textures: Results From an in vitro Study. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.868347"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Cometta, S., Bock, N., Suresh, S., Dargaville, T.R., and Hutmacher, D.W. (2021). Antibacterial Albumin-Tannic Acid Coatings for Scaffold-Guided Breast Reconstruction. Front. Bioeng. Biotechnol., 9.","DOI":"10.3389\/fbioe.2021.638577"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.actbio.2020.11.020","article-title":"Review of silicone surface modification techniques and coatings for antibacterial\/antimicrobial applications to improve breast implant surfaces","volume":"121","author":"Lam","year":"2020","journal-title":"Acta Biomater."},{"key":"ref_51","first-page":"e2389","article-title":"A Simple and Low-cost Method of Sleeve to Insert Silicone Gel Breast Implants","volume":"7","author":"Panczel","year":"2019","journal-title":"Plast. Reconstr. Surg.-Glob. Open"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1097\/00006534-198107000-00010","article-title":"Capsules, infection, and intraluminal antibiotics","volume":"68","author":"Burkhardt","year":"1981","journal-title":"Plast Reconstr. Surg."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1097\/00006534-198107000-00007","article-title":"Does infection play a role in breast capsular contracture?","volume":"68","author":"Shah","year":"1981","journal-title":"Plast Reconstr. Surg."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.cps.2020.09.009","article-title":"Adverse Events Associated with Breast Implants: The Role of Bacterial Infection and Biofilm","volume":"48","author":"Moon","year":"2021","journal-title":"Clin. Plast. Surg."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1007\/s00266-020-01816-w","article-title":"Subclinical Infection of the Silicone Breast Implant Surface as a Possible Cause of Capsular Contracture","volume":"44","author":"Virden","year":"2020","journal-title":"Aesthetic Plast. Surg."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1053\/aq.1999.v19.97038","article-title":"Bacterial biofilms: A cause for accelerated capsular contracture?","volume":"19","author":"Deva","year":"1999","journal-title":"Aesthetic Surg. J."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"917","DOI":"10.2217\/fmb.10.56","article-title":"Current concepts in biofilm formation of Staphylococcus epidermidis","volume":"5","author":"Fey","year":"2010","journal-title":"Futur. Microbiol."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Moris, V., Lam, M., Amoureux, L., Magallon, A., Guilloteau, A., Maldiney, T., Zwetyenga, N., Falentin-Daudre, C., and Neuwirth, C. (2022). What is the best technic to dislodge Staphylococcus epidermidis biofilm on medical implants?. BMC Microbiol., 22.","DOI":"10.1186\/s12866-022-02606-x"},{"key":"ref_59","first-page":"1144","article-title":"Biofilm Formation on Breast Implant Surfaces by Major Gram-Positive Bacterial Pathogens","volume":"41","author":"Albuquerque","year":"2020","journal-title":"Aesthetic Surg. J."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"5426","DOI":"10.1021\/acs.langmuir.3c00091","article-title":"Influence of Surface Roughness, Nanostructure, and Wetting on Bacterial Adhesion","volume":"39","author":"Mu","year":"2023","journal-title":"Langmuir"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"322e","DOI":"10.1097\/PRS.0000000000005816","article-title":"Rough Textured Silicone Implants, Bacterial Biofilms, and Capsular Contracture","volume":"144","author":"Brauman","year":"2019","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1007\/s00266-018-1234-7","article-title":"Bacterial Adhesion and Biofilm Formation on Textured Breast Implant Shell Materials","volume":"43","author":"James","year":"2018","journal-title":"Aesthetic Plast. Surg."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"21192","DOI":"10.1021\/acsami.9b22621","article-title":"Submicrometer-Sized Roughness Suppresses Bacteria Adhesion","volume":"12","author":"Encinas","year":"2020","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Yoda, I., Koseki, H., Tomita, M., Shida, T., Horiuchi, H., Sakoda, H., and Osaki, M. (2014). Effect of surface roughness of biomaterials on Staphylococcus epidermidis adhesion. BMC Microbiol., 14.","DOI":"10.1186\/s12866-014-0234-2"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Alessandri-Bonetti, M., Jeong, T., Vaienti, L., De La Cruz, C., Gimbel, M.L., Nguyen, V.T., and Egro, F.M. (2023). The Role of Microorganisms in the Development of Breast Implant-Associated Anaplastic Large Cell Lymphoma. Pathogens, 12.","DOI":"10.3390\/pathogens12020313"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"D638","DOI":"10.1093\/nar\/gkac1000","article-title":"The STRING database in 2023: Protein\u2013protein association networks and functional enrichment analyses for any sequenced genome of interest","volume":"51","author":"Szklarczyk","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1111\/exd.12749","article-title":"Filaggrin-2 barrier protein inversely varies with skin inflammation","volume":"24","author":"Seykora","year":"2015","journal-title":"Exp. Dermatol."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Hansmann, B., Schr\u00f6der, J.-M., and Gerstel, U. (2015). Skin-Derived C-Terminal Filaggrin-2 Fragments Are Pseudomonas aeruginosa-Directed Antimicrobials Targeting Bacterial Replication. PLoS Pathog., 11.","DOI":"10.1371\/journal.ppat.1005159"},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Wu, Z., Hansmann, B., Meyer-Hoffert, U., Gl\u00e4ser, R., and Schr\u00f6der, J.-M. (2009). Molecular identification and expression analysis of Filaggrin-2, a member of the S100 fused-type protein family. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0005227"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1111\/j.1600-0625.2012.01449.x","article-title":"Murine filaggrin-2 is involved in epithelial barrier function and down-regulated in metabolically induced skin barrier dysfunction","volume":"21","author":"Hansmann","year":"2012","journal-title":"Exp. Dermatol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/mBio.02945-19","article-title":"Universal dermal microbiome in human skin","volume":"11","author":"Bay","year":"2020","journal-title":"mBio"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1007\/s10096-013-1975-9","article-title":"Ralstonia spp.: Emerging global opportunistic pathogens","volume":"33","author":"Ryan","year":"2014","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Park, J.Y., and Seo, K.S. (2023). Staphylococcus aureus Infection. Food Microbiol. Fundam. Front., 555\u2013584. Available online: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK441868\/.","DOI":"10.1128\/9781555819972.ch21"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Andrey, D.O., Jousselin, A., Villanueva, M., Renzoni, A., Monod, A., Barras, C., Rodriguez, N., and Kelley, W.L. (2015). Impact of the Regulators SigB, Rot, SarA and sarS on the Toxic Shock Tst Promoter and TSST-1 Expression in Staphylococcus aureus. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0135579"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1002\/jbm.10006","article-title":"In catheter infections by Staphylococcus epidermidis the intercellular adhesion (ica) locus is a molecular marker of the virulent slime-producing strains","volume":"59","author":"Arciola","year":"2001","journal-title":"J. Biomed. Mater. Res."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"7","DOI":"10.3389\/fcimb.2015.00007","article-title":"Polysaccharide intercellular adhesin in biofilm: Structural and regulatory aspects","volume":"5","author":"Arciola","year":"2015","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"3301","DOI":"10.1128\/JB.01496-08","article-title":"Regulation of Superoxide Dismutase (sod) Genes by SarA in Staphylococcus aureus","volume":"191","author":"Ballal","year":"2009","journal-title":"J. Bacteriol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S1369-5274(00)00199-5","article-title":"The role of gamma interferon in antimicrobial immunity","volume":"4","author":"Shtrichman","year":"2001","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1172\/JCI18991","article-title":"TNF-\u03b1 is a critical negative regulator of type 1 immune activation during intracellular bacterial infection","volume":"113","author":"Zganiacz","year":"2004","journal-title":"J. Clin. Investig."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"4196","DOI":"10.4049\/jimmunol.1202688","article-title":"IL-1\u03b2 Promotes Antimicrobial Immunity in Macrophages by Regulating TNFR Signaling and caspase-3 activation","volume":"190","author":"Jayaraman","year":"2013","journal-title":"J. Immunol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1002\/eji.200839090","article-title":"IL-17 and anti-bacterial immunity: Protection versus tissue damage","volume":"39","author":"Cooper","year":"2009","journal-title":"Eur. J. Immunol."},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Spiegel, C., Nogler, M., and Cora\u00e7a-Huber, D.C. (2022). Sterilization Procedures for Titanium Alloy Surfaces Leads to Higher Expression of Biofilm-Related Staphylococcus aureus Genes. Antibiotics, 11.","DOI":"10.3390\/antibiotics11111647"},{"key":"ref_83","unstructured":"Anderson, J.M. (2008). Principles of Regenerative Medicine, Academic Press."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.smim.2007.11.004","article-title":"Foreign body reaction to biomaterials","volume":"Volume 20","author":"Anderson","year":"2008","journal-title":"Seminars in Immunology"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"348","DOI":"10.3201\/eid0702.010241","article-title":"New disinfection and sterilization methods","volume":"7","author":"Rutala","year":"2001","journal-title":"Emerg. Infect. Dis."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"11847","DOI":"10.1038\/s41598-017-10252-x","article-title":"GATA3 regulates FLG and FLG2 expression in human primary keratinocytes","volume":"7","author":"Zeitvogel","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1111\/exd.13317","article-title":"Filaggrin has evolved from an \u201cS100 fused-type protein\u201d (SFTP) gene present in a common ancestor of amphibians and mammals","volume":"26","author":"Mlitz","year":"2017","journal-title":"Exp. Dermatol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.21873\/invivo.12882","article-title":"Effect of Breast Silicone Implant Topography on Bacterial Attachment and Growth: An In Vitro Study","volume":"36","author":"Lee","year":"2022","journal-title":"In Vivo"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"21","DOI":"10.4236\/jbnb.2021.123003","article-title":"An Impact of Different Silicone Breast Implants on the Bacterial Attachment and Growth","volume":"12","author":"Nam","year":"2021","journal-title":"J. Biomater. Nanobiotechnol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1097\/PRS.0000000000001682","article-title":"Breast implant-associated infections: The role of the national surgical quality improvement program and the local microbiome","volume":"136","author":"Cohen","year":"2015","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_91","doi-asserted-by":"crossref","unstructured":"Palubicka, A., Jaworski, R., Wekwejt, M., Swieczko-Zurek, B., Pikula, M., Jaskiewicz, J., and Zielinski, J. (2019). surgical site infection after breast surgery: A retrospective analysis of 5-year postoperative data from a single center in Poland. Medicina, 55.","DOI":"10.20944\/preprints201907.0076.v1"},{"key":"ref_92","first-page":"e2590","article-title":"Recommendations for the Use of Antibiotics in Primary and Secondary Esthetic Breast Surgery","volume":"8","author":"Prantl","year":"2020","journal-title":"Plast. Reconstr. Surg.-Glob. Open"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"2345","DOI":"10.1007\/s00266-023-03453-5","article-title":"Use of Antimicrobial Irrigation and Incidence of Capsular Contracture in Breast Augmentation and Immediate Implant-Based Breast Reconstruction","volume":"47","author":"Federica","year":"2023","journal-title":"Aesthetic Plast. Surg."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1089\/sur.2019.116","article-title":"Peri-operative antibiotic prophylaxis does not reduce surgical site infection in breast cancer","volume":"21","author":"Zhang","year":"2020","journal-title":"Surg. Infect."},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"Heberle, H., Meirelles, G.V., Da Silva, F.R., Telles, G.P., and Minghim, R. (2015). InteractiVenn: A web-based tool for the analysis of sets through venn diagrams. BMC Bioinform., 16.","DOI":"10.1186\/s12859-015-0611-3"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"2628","DOI":"10.1093\/bioinformatics\/btz931","article-title":"ShinyGO: A graphical gene-set enrichment tool for animals and plants","volume":"36","author":"Ge","year":"2020","journal-title":"Bioinformatics"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1093\/nar\/gkg034","article-title":"STRING: A database of predicted functional associations between proteins","volume":"31","author":"Huynen","year":"2003","journal-title":"Nucleic Acids Res."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"W566","DOI":"10.1093\/nar\/gkv468","article-title":"ClustVis: A web tool for visualizing clustering of multivariate data using Principal Component Analysis and heatmap","volume":"43","author":"Metsalu","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_99","doi-asserted-by":"crossref","unstructured":"Davis, N.M., Proctor, D.M., Holmes, S.P., Relman, D.A., and Callahan, B.J. (2018). Simple statistical identification and removal of contaminant sequences in marker-gene and metagenomics data. Microbiome, 6.","DOI":"10.1186\/s40168-018-0605-2"},{"key":"ref_100","doi-asserted-by":"crossref","unstructured":"McMurdie, P.J., and Holmes, S. (2013). phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census Data. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0061217"},{"key":"ref_101","doi-asserted-by":"crossref","unstructured":"Wickham, H. (2016). ggplot2: Elegant Graphics for Data Analysis, Springer. Available online: https:\/\/ggplot2.tidyverse.org.","DOI":"10.1007\/978-3-319-24277-4"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.jmbbm.2017.06.030","article-title":"Functional biocompatibility testing of silicone breast implants and a novel classification system based on surface roughness","volume":"75","author":"Barr","year":"2017","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"NP324","DOI":"10.1093\/asj\/sjz369","article-title":"Intra- and Inter-Shell Roughness Variability of Breast Implant Surfaces","volume":"40","author":"Atlan","year":"2020","journal-title":"Aesthetic Surg. J."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/s00266-015-0603-8","article-title":"Characterization of Breast Implant Surfaces, Shapes, and Biomechanics: A Comparison of High Cohesive Anatomically Shaped Textured Silicone, Breast Implants from Three Different Manufacturers","volume":"40","author":"Atlan","year":"2016","journal-title":"Aesthetic Plast. Surg."},{"key":"ref_105","doi-asserted-by":"crossref","unstructured":"Grimus, V., Cora\u00e7a-Huber, D.C., Steixner, S.J.M., and Nagl, M. (2021). Activity of N-Chlorotaurine against Long-Term Biofilms of Bacteria and Yeasts. 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