{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T06:21:06Z","timestamp":1769840466873,"version":"3.49.0"},"reference-count":44,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T00:00:00Z","timestamp":1710374400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Anaphylactic shock (AS) is the most severe form of acute systemic hypersensitivity reaction. Although epinephrine can restore patients\u2019 hemodynamics, it might also be harmful, supporting the need for adjuvant treatment. We therefore investigated whether NButGT, enhancing O-GlcNAcylation and showing beneficial effects in acute heart failure might improve AS therapy. Ovalbumin-sensitized rats were randomly allocated to six groups: control (CON), shock (AS), shock treated with NButGT alone before (AS+pre-Nbut) or after (AS+post-Nbut) AS onset, shock treated with epinephrine alone (AS+EPI) and shock group treated with combination of epinephrine and NButGT (AS+EPI+preNBut). Induction of shock was performed with an intravenous (IV) ovalbumin. Cardiac protein and cycling enzymes O-GlcNAcylation levels, mean arterial pressure (MAP), heart rate, cardiac output (CO), left ventricle shortening fraction (LVSF), mitochondrial respiration, and lactatemia were evaluated using Western blotting experiments, invasive arterial monitoring, echocardiography, mitochondrial oximetry and arterial blood samples. AS decreased MAP (\u221277%, p &lt; 0.001), CO (\u221290%, p &lt; 0.001) and LVSF (\u221230%, p &lt; 0.05). Epinephrine improved these parameters and, in particular, rats did not die in 15 min. But, cardiac mitochondrial respiration remained impaired (complexes I + II \u221229%, p &lt; 0.05 and II \u221240%, p &lt; 0.001) with hyperlactatemia. NButGT pretreatment (AS+pre-Nbut) efficiently increased cardiac O-GlcNAcylation level as compared to the AS+post-Nbut group. Compared to epinephrine alone, the adjunction of NButGT significantly improved CO, LVSF and mitochondrial respiration. MAP was not significantly increased but lactatemia decreased more markedly. Pretreatment with NButGT increases O-GlcNAcylation of cardiac proteins and has an additive effect on epinephrine, improving cardiac output and mitochondrial respiration and decreasing blood lactate levels. This new therapy might be useful when the risk of AS cannot be avoided.<\/jats:p>","DOI":"10.3390\/ijms25063316","type":"journal-article","created":{"date-parts":[[2024,3,15]],"date-time":"2024-03-15T09:32:30Z","timestamp":1710495150000},"page":"3316","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["NButGT Reinforces the Beneficial Effects of Epinephrine on Cardiac Mitochondrial Respiration, Lactatemia and Cardiac Output in Experimental Anaphylactic Shock"],"prefix":"10.3390","volume":"25","author":[{"given":"Walid","family":"Oulehri","sequence":"first","affiliation":[{"name":"P\u00f4le Anesth\u00e9sie, R\u00e9animation Chirurgicale, H\u00f4pitaux Universitaires de Strasbourg, 67091 Strasbourg, France"},{"name":"Facult\u00e9 de M\u00e9decine de Strasbourg, UR 3072 Institut de Physiologie, FMTS (F\u00e9d\u00e9ration de M\u00e9decine Translationnelle de Strasbourg), Universit\u00e9 de Strasbourg, 67091 Strasbourg, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4722-9422","authenticated-orcid":false,"given":"Antoine","family":"Persello","sequence":"additional","affiliation":[{"name":"Institut du Thorax, Universit\u00e9 de Nantes, CNRS, INSERM, 44000 Nantes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelique","family":"Blangy-Letheule","sequence":"additional","affiliation":[{"name":"Institut du Thorax, Universit\u00e9 de Nantes, CNRS, INSERM, 44000 Nantes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Tacquard","sequence":"additional","affiliation":[{"name":"P\u00f4le Anesth\u00e9sie, R\u00e9animation Chirurgicale, H\u00f4pitaux Universitaires de Strasbourg, 67091 Strasbourg, France"},{"name":"Facult\u00e9 de M\u00e9decine de Strasbourg, UR 3072 Institut de Physiologie, FMTS (F\u00e9d\u00e9ration de M\u00e9decine Translationnelle de Strasbourg), Universit\u00e9 de Strasbourg, 67091 Strasbourg, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bertrand","family":"Rozec","sequence":"additional","affiliation":[{"name":"Institut du Thorax, Universit\u00e9 de Nantes, CNRS, INSERM, 44000 Nantes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3557-2642","authenticated-orcid":false,"given":"Anne-Laure","family":"Charles","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine de Strasbourg, UR 3072 Institut de Physiologie, FMTS (F\u00e9d\u00e9ration de M\u00e9decine Translationnelle de Strasbourg), Universit\u00e9 de Strasbourg, 67091 Strasbourg, France"},{"name":"Service de Physiologie et d\u2019Explorations Fonctionnelles, P\u00f4le de Pathologie Thoracique, H\u00f4pitaux Universitaires de Strasbourg, 67091 Strasbourg, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Geny","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine de Strasbourg, UR 3072 Institut de Physiologie, FMTS (F\u00e9d\u00e9ration de M\u00e9decine Translationnelle de Strasbourg), Universit\u00e9 de Strasbourg, 67091 Strasbourg, France"},{"name":"Service de Physiologie et d\u2019Explorations Fonctionnelles, P\u00f4le de Pathologie Thoracique, H\u00f4pitaux Universitaires de Strasbourg, 67091 Strasbourg, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1370-6155","authenticated-orcid":false,"given":"Benjamin","family":"Lauzier","sequence":"additional","affiliation":[{"name":"Institut du Thorax, Universit\u00e9 de Nantes, CNRS, INSERM, 44000 Nantes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul Michel","family":"Mertes","sequence":"additional","affiliation":[{"name":"P\u00f4le Anesth\u00e9sie, R\u00e9animation Chirurgicale, H\u00f4pitaux Universitaires de Strasbourg, 67091 Strasbourg, France"},{"name":"Facult\u00e9 de M\u00e9decine de Strasbourg, UR 3072 Institut de Physiologie, FMTS (F\u00e9d\u00e9ration de M\u00e9decine Translationnelle de Strasbourg), Universit\u00e9 de Strasbourg, 67091 Strasbourg, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Collange","sequence":"additional","affiliation":[{"name":"P\u00f4le Anesth\u00e9sie, R\u00e9animation Chirurgicale, H\u00f4pitaux Universitaires de Strasbourg, 67091 Strasbourg, France"},{"name":"Facult\u00e9 de M\u00e9decine de Strasbourg, UR 3072 Institut de Physiologie, FMTS (F\u00e9d\u00e9ration de M\u00e9decine Translationnelle de Strasbourg), Universit\u00e9 de Strasbourg, 67091 Strasbourg, 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