{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:53:55Z","timestamp":1760144035121,"version":"build-2065373602"},"reference-count":45,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,3,15]],"date-time":"2024-03-15T00:00:00Z","timestamp":1710460800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Romanian Ministry of Education and Research, CNCS\u2014UEFISCDI","award":["PN-III-P4-ID-PCE-2020-1756","PN-III-P2-2.1-PTE-2021-0176","#R03ES020446"],"award-info":[{"award-number":["PN-III-P4-ID-PCE-2020-1756","PN-III-P2-2.1-PTE-2021-0176","#R03ES020446"]}]},{"name":"Ministry of Research, Innovation and Digitization, CCCDI\u2014Executive Agency for Higher Education, Research, Development &amp; Innovation Funding (UEFISCDI)","award":["PN-III-P4-ID-PCE-2020-1756","PN-III-P2-2.1-PTE-2021-0176","#R03ES020446"],"award-info":[{"award-number":["PN-III-P4-ID-PCE-2020-1756","PN-III-P2-2.1-PTE-2021-0176","#R03ES020446"]}]},{"name":"National Institute of Environmental Health Sciences (NIEHS)","award":["PN-III-P4-ID-PCE-2020-1756","PN-III-P2-2.1-PTE-2021-0176","#R03ES020446"],"award-info":[{"award-number":["PN-III-P4-ID-PCE-2020-1756","PN-III-P2-2.1-PTE-2021-0176","#R03ES020446"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Previous studies have shown that inorganic arsenic (iAs) exposure may be associated with genotoxic and cytotoxic effects. The aim of this study was to evaluate the relationship between several polymorphisms in AS3MT and APOE genes and urinary As and the relationship between these polymorphisms and pregnancy loss. We determined urinary As concentrations and performed genotyping analysis in 50 cases of spontaneous pregnancy loss and 50 controls, matched to cases on gestational age. The most frequently identified AS3MT polymorphisms in both cases and controls were in rs10748835 (80% cases and 68% controls), rs3740400 (78% cases and 64% controls), rs7085104 (74% cases and 48% controls), and rs1046778 (62% cases and 54% controls). We identified 30 different haplotypes in AS3MT SNPs, with four predominant haplotypes (&gt;8%). Cases with Haplotype 1 had four-fold higher urinary DMA and two-fold higher MMA concentration than those without this haplotype, the MMA levels were lower in cases and controls with Haplotype 4 compared to Haplotype 1, and the DMA levels were significantly lower in cases with Haplotype 4 compared to Haplotype 3. Cases with Haplotype 1 had higher levels of all analyzed biomarkers, suggesting that Haplotype 1 may be associated with greater exposure to iAs and tobacco smoke. Our results suggest the importance of the AS3MT gene in iAs metabolism among pregnant women with low-level drinking water iAs exposure.<\/jats:p>","DOI":"10.3390\/ijms25063349","type":"journal-article","created":{"date-parts":[[2024,3,15]],"date-time":"2024-03-15T09:32:30Z","timestamp":1710495150000},"page":"3349","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Arsenic Methyltransferase and Apolipoprotein E Polymorphism in Pregnant Women Exposed to Inorganic Arsenic in Drinking Water in Western Romania"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5806-0220","authenticated-orcid":false,"given":"Laura Ancuta","family":"Pop","sequence":"first","affiliation":[{"name":"Research Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 8 Victor Babes Street, 400012 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5828-1325","authenticated-orcid":false,"given":"Ioana","family":"Berindan-Neagoe","sequence":"additional","affiliation":[{"name":"Research Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 8 Victor Babes Street, 400012 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0028-5494","authenticated-orcid":false,"given":"Michael S.","family":"Bloom","sequence":"additional","affiliation":[{"name":"Department of Global and Community Health, George Mason University, 4400 University Dr, Fairfax, VA 22030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5314-0539","authenticated-orcid":false,"given":"Iulia Adina","family":"Neamtiu","sequence":"additional","affiliation":[{"name":"Health Department, Environmental Health Center Part of ALS, 58 Busuiocului Street, 400240 Cluj-Napoca, Romania"},{"name":"Faculty of Environmental Science and Engineering, Babes-Bolyai University, 30 Fantanele Street, 400294 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cecilia","family":"Bica","sequence":"additional","affiliation":[{"name":"Research Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 8 Victor Babes Street, 400012 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugen S.","family":"Gurzau","sequence":"additional","affiliation":[{"name":"Research Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 8 Victor Babes Street, 400012 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1016\/S0140-6736(21)00682-6","article-title":"Miscarriage matters: The epidemiological, physical, psychological, and economic costs of early pregnancy loss","volume":"397","author":"Quenby","year":"2021","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.ejogrb.2016.07.514","article-title":"Maternal pre-pregnancy risk factors for miscarriage from a prevention perspective: A cohort study in China","volume":"206","author":"Zhou","year":"2016","journal-title":"Eur. 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