{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T02:36:33Z","timestamp":1771209393159,"version":"3.50.1"},"reference-count":41,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,3,16]],"date-time":"2024-03-16T00:00:00Z","timestamp":1710547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fiocruz, Fiotec and Plataforma de Pesquisa Cl\u00ednica\u2014VPPCB","award":["VPPIS-004-FIO-18-58"],"award-info":[{"award-number":["VPPIS-004-FIO-18-58"]}]},{"name":"Fiocruz, Fiotec and Plataforma de Pesquisa Cl\u00ednica\u2014VPPCB","award":["VPPCB-007-FIO-18-2-113"],"award-info":[{"award-number":["VPPCB-007-FIO-18-2-113"]}]},{"name":"Fiocruz, Fiotec and Plataforma de Pesquisa Cl\u00ednica\u2014VPPCB","award":["445940\/2020-4"],"award-info":[{"award-number":["445940\/2020-4"]}]},{"name":"Gera\u00e7\u00e3o de Conhecimento","award":["VPPIS-004-FIO-18-58"],"award-info":[{"award-number":["VPPIS-004-FIO-18-58"]}]},{"name":"Gera\u00e7\u00e3o de Conhecimento","award":["VPPCB-007-FIO-18-2-113"],"award-info":[{"award-number":["VPPCB-007-FIO-18-2-113"]}]},{"name":"Gera\u00e7\u00e3o de Conhecimento","award":["445940\/2020-4"],"award-info":[{"award-number":["445940\/2020-4"]}]},{"DOI":"10.13039\/501100003593","name":"Brazilian National Council for Scientific and Technological Development (CNPq)","doi-asserted-by":"publisher","award":["VPPIS-004-FIO-18-58"],"award-info":[{"award-number":["VPPIS-004-FIO-18-58"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Brazilian National Council for Scientific and Technological Development (CNPq)","doi-asserted-by":"publisher","award":["VPPCB-007-FIO-18-2-113"],"award-info":[{"award-number":["VPPCB-007-FIO-18-2-113"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Brazilian National Council for Scientific and Technological Development (CNPq)","doi-asserted-by":"publisher","award":["445940\/2020-4"],"award-info":[{"award-number":["445940\/2020-4"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Chronic myeloid leukemia (CML) is a type of leukemia whose main genetic marker is the reciprocal translocation that leads to the production of the BCR::ABL1 oncoprotein. The expression of some genes may interfere with the progression and development of leukemias. MicroRNAs are small non-coding RNAs that have the potential to alter the expression of some genes and may be correlated with some types of leukemia and could be used as biomarkers in the diagnosis and prognosis of patients. Therefore, this project carried out an analysis of microRNA-type plasma biomarkers in patients with chronic myeloid leukemia at unique points, including follow-up analysis of patients from the Erasto Gaertner Hospital. 35 microRNAs were analyzed in different cohorts. Inside those groups, 70 samples were analyzed at unique points and 11 patients in a follow-up analysis. Statistically different results were found for microRNA-7-5p, which was found to be upregulated in patients with high expression of the BCR::ABL1 transcript when compared to healthy controls. This microRNA also had evidence of behavior related to BCR::ABL1 when analyzed in follow-up, but strong evidence was not found. In this way, this work obtained results that may lead to manifestations of a relationship between miR-7-5p and chronic myeloid leukemia, and evaluations of possible microRNAs that are not related to this pathology.<\/jats:p>","DOI":"10.3390\/ijms25063363","type":"journal-article","created":{"date-parts":[[2024,3,18]],"date-time":"2024-03-18T05:14:07Z","timestamp":1710738847000},"page":"3363","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Screening of microRNAs in Chronic Myeloid Leukemia: A Clinical Evaluation"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8248-0831","authenticated-orcid":false,"given":"Denise Kusma","family":"Wosniaki","sequence":"first","affiliation":[{"name":"Laboratory for Applied Science and Technology in Health, Carlos Chagas Institute, Oswaldo Cruz Foundation (Fiocruz), Curitiba 81350-010, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2260-7949","authenticated-orcid":false,"given":"Anelis Maria","family":"Marin","sequence":"additional","affiliation":[{"name":"Laboratory for Applied Science and Technology in Health, Carlos Chagas Institute, Oswaldo Cruz Foundation (Fiocruz), Curitiba 81350-010, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafaela Noga","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory for Applied Science and Technology in Health, Carlos Chagas Institute, Oswaldo Cruz Foundation (Fiocruz), Curitiba 81350-010, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela Marino","family":"Koerich","sequence":"additional","affiliation":[{"name":"Laboratory for Applied Science and Technology in Health, Carlos Chagas Institute, Oswaldo Cruz Foundation (Fiocruz), Curitiba 81350-010, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo Cili\u00e3o","family":"Munhoz","sequence":"additional","affiliation":[{"name":"Erasto Gaertner Hospital, Curitiba 81520-060, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Samuel de Holanda","family":"Farias","sequence":"additional","affiliation":[{"name":"Erasto Gaertner Hospital, Curitiba 81520-060, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miriam Perlingeiro","family":"Beltrame","sequence":"additional","affiliation":[{"name":"Erasto Gaertner Hospital, Curitiba 81520-060, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9397-4127","authenticated-orcid":false,"given":"Dalila Luciola","family":"Zanette","sequence":"additional","affiliation":[{"name":"Laboratory for Applied Science and Technology in Health, Carlos Chagas Institute, Oswaldo Cruz Foundation (Fiocruz), Curitiba 81350-010, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5166-2897","authenticated-orcid":false,"given":"Mateus N\u00f3brega","family":"Aoki","sequence":"additional","affiliation":[{"name":"Laboratory for Applied Science and Technology in Health, Carlos Chagas Institute, Oswaldo Cruz Foundation (Fiocruz), Curitiba 81350-010, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,16]]},"reference":[{"key":"ref_1","first-page":"7008","article-title":"MicroRNA-577 Promotes the Sensitivity of Chronic Myeloid Leukemia Cells to Imatinib by Targeting NUP160","volume":"23","author":"Zhang","year":"2019","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1727","DOI":"10.21037\/apm-20-343","article-title":"Systematic Analysis Reveals a Lncrna-Mirna-Mrna Network Associated with Dasatinib Resistance in Chronic Myeloid Leukemia","volume":"10","author":"Luo","year":"2021","journal-title":"Ann. Palliat. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3175","DOI":"10.2147\/CMAR.S232752","article-title":"Monitoring of Minimal Residual Disease (Mrd) in Chronic Myeloid Leukemia: Recent Advances","volume":"12","author":"Cumbo","year":"2020","journal-title":"Cancer Manag. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1007\/s11899-018-0474-6","article-title":"Chronic Myeloid Leukemia: Beyond BCR-ABL1","volume":"13","author":"Zhou","year":"2018","journal-title":"Curr. Hematol. Malig. Rep."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"100825","DOI":"10.1016\/j.blre.2021.100825","article-title":"Chronic Myeloid Leukemia: Modern Therapies, Current Challenges and Future Directions","volume":"49","author":"Osman","year":"2021","journal-title":"Blood Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"379","DOI":"10.2478\/raon-2021-0042","article-title":"MitomiRs: Their Roles in Mitochondria and Importance in Cancer Cell Metabolism","volume":"55","author":"Rencelj","year":"2021","journal-title":"Radiol. Oncol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Anelli, L., Zagaria, A., Specchia, G., Musto, P., and Albano, F. (2021). Dysregulation of Mirna in Leukemia: Exploiting Mirna Expression Profiles as Biomarkers. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22137156"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"679022","DOI":"10.3389\/fonc.2021.679022","article-title":"Clinical Applications of MicroRNAs in Acute Myeloid Leukemia: A Mini-Review","volume":"11","author":"Bhatnagar","year":"2021","journal-title":"Front. Oncol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1290","DOI":"10.1182\/blood-2016-10-697698","article-title":"MicroRNAs and Acute Myeloid Leukemia: Therapeutic Implications and Emerging Concepts","volume":"130","author":"Wallace","year":"2017","journal-title":"Blood"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1038\/leu.2013.361","article-title":"Differential Expression of MiR-17~92 Identifies BCL2 as a Therapeutic Target in BCR-ABL-Positive B-Lineage Acute Lymphoblastic Leukemia","volume":"28","author":"Scherr","year":"2014","journal-title":"Leukemia"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.leukres.2016.04.006","article-title":"The MiR-17~92 Cluster Contributes to MLL Leukemia through the Repression of MEIS1 Competitor PKNOX1","volume":"46","author":"Mian","year":"2016","journal-title":"Leuk. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3710","DOI":"10.1073\/pnas.0914900107","article-title":"Aberrant Overexpression and Function of the MiR-17-92 Cluster in MLL-Rearranged Acute Leukemia","volume":"107","author":"Mi","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Chocholska, S., Zarobkiewicz, M., Szyma\u0144ska, A., Lehman, N., Wo\u015b, J., and Bojarska-Junak, A. (2023). Prognostic Value of the MiR-17~92 Cluster in Chronic Lymphocytic Leukemia. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24021705"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Wang, X., Gao, X., Tian, J., Zhang, R., Qiao, Y., Hua, X., and Shi, G. (2020). LINC00261 Inhibits Progression of Pancreatic Cancer by Down-Regulating MiR-23a-3p. Arch. Biochem. Biophys., 689.","DOI":"10.1016\/j.abb.2020.108469"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1016\/j.biopha.2018.11.065","article-title":"MiR-23a-3p Acts as an Oncogene and Potential Prognostic Biomarker by Targeting PNRC2 in RCC","volume":"110","author":"Quan","year":"2019","journal-title":"Biomed. Pharmacother."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Lee, Y., Kim, S.J., Choo, J., Heo, G., Yoo, J.-W., Jung, Y., Rhee, S.H., and Im, E. (2020). MiR-23a-3p Is a Key Regulator of IL-17C-Induced Tumor Angiogenesis in Colorectal Cancer. Cells, 9.","DOI":"10.3390\/cells9061363"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"203","DOI":"10.21037\/atm-22-97","article-title":"MiR-93-5p Regulates Tumorigenesis and Tumor Immunity by Targeting PD-L1\/CCND1 in Breast Cancer","volume":"10","author":"Yang","year":"2022","journal-title":"Ann. Transl. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.yexcr.2017.05.007","article-title":"MiR-93-5p Inhibits the EMT of Breast Cancer Cells via Targeting MKL-1 and STAT3","volume":"357","author":"Xiang","year":"2017","journal-title":"Exp. Cell Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1604","DOI":"10.1002\/cam4.1860","article-title":"LncRNA H19 Interacted with MiR-130a-3p and MiR-17-5p to Modify Radio-Resistance and Chemo-Sensitivity of Cardiac Carcinoma Cells","volume":"8","author":"Jia","year":"2019","journal-title":"Cancer Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s40164-020-00183-1","article-title":"Expression Differences of MiR-142-5p between Treatment-Na\u00efve Chronic Myeloid Leukemia Patients Responding and Non-Responding to Imatinib Therapy Suggest a Link to Oncogenic ABL2, SRI, CKIT and MCL1 Signaling Pathways Critical for Development of Therapy Resistance","volume":"9","author":"Bruckmueller","year":"2020","journal-title":"Exp. Hematol. Oncol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"10924","DOI":"10.1038\/s41598-022-15162-1","article-title":"Mutations in the MiR-142 Gene Are Not Common in Myeloproliferative Neoplasms","volume":"12","author":"Tire","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_22","first-page":"881","article-title":"[MiR-148b-3p Inhibits the Proliferation and Autophagy of Acute Myeloid Leukemia Cells by Targeting ATG14]","volume":"37","author":"Jiang","year":"2021","journal-title":"Xi Bao Yu Fen. Zi Mian Yi Xue Za Zhi"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.3389\/fonc.2019.01076","article-title":"Exosomes Derived From MicroRNA-148b-3p-Overexpressing Human Umbilical Cord Mesenchymal Stem Cells Restrain Breast Cancer Progression","volume":"9","author":"Yuan","year":"2019","journal-title":"Front. Oncol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"4501","DOI":"10.1007\/s13277-015-3092-0","article-title":"Circulating MiR-375 and MiR-199a-3p as Potential Biomarkers for the Diagnosis of Hepatocellular Carcinoma","volume":"36","author":"Yin","year":"2015","journal-title":"Tumor Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1159\/000524158","article-title":"MiR-199a-3p Overexpression Suppressed Cell Proliferation and Sensitized Chronic Myeloid Leukaemia Cells to Imatinib by Inhibiting MTOR Signalling","volume":"145","author":"Liu","year":"2022","journal-title":"Acta Haematol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2164","DOI":"10.1111\/j.1582-4934.2010.01213.x","article-title":"Down-Regulated MiR-331-5p and MiR-27a Are Associated with Chemotherapy Resistance and Relapse in Leukaemia","volume":"15","author":"Feng","year":"2011","journal-title":"J. Cell Mol. Med."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Wang, X., Tang, S., Le, S.-Y., Lu, R., Rader, J.S., Meyers, C., and Zheng, Z.-M. (2008). Aberrant Expression of Oncogenic and Tumor-Suppressive MicroRNAs in Cervical Cancer Is Required for Cancer Cell Growth. PLoS ONE, 3.","DOI":"10.1371\/journal.pone.0002557"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4780","DOI":"10.1158\/1078-0432.CCR-12-2728","article-title":"Clinical Significance and Prognostic Value of MicroRNA Expression Signatures in Hepatocellular Carcinoma","volume":"19","author":"Wei","year":"2013","journal-title":"Clin. Cancer Res."},{"key":"ref_29","unstructured":"Kristina Gregory, N., Hema Sundar, M., Drazer, M., Maness, L., Fred and Pamela Buffett Cancer Center Leland Metheny, Mohan, S., Moore, J.O., Oehler, V., Pratz, K., and Pusic, I. (2023). NCCN Guidelines Version 2.2024 Chronic Myeloid Leukemia Continue NCCN Guidelines Panel Disclosures, National Comprehensive Cancer Network."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"42683","DOI":"10.18632\/oncotarget.7977","article-title":"MicroRNAs Mediated Regulation of MAPK Signaling Pathways in Chronic Myeloid Leukemia","volume":"7","author":"Chakraborty","year":"2016","journal-title":"Oncotarget"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s11010-013-1958-2","article-title":"Studies on MicroRNAs That Are Correlated with the Cancer Stem Cells in Chronic Myeloid Leukemia","volume":"390","author":"Zhu","year":"2014","journal-title":"Mol. Cell Biochem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1186\/s12935-020-01376-8","article-title":"LncRNA ANRIL\/MiR-7-5p\/TCF4 Axis Contributes to the Progression of T Cell Acute Lymphoblastic Leukemia","volume":"20","author":"Li","year":"2020","journal-title":"Cancer Cell Int."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"588893","DOI":"10.3389\/fonc.2020.588893","article-title":"MicroRNA-7 Regulates Migration and Chemoresistance in Non-Hodgkin Lymphoma Cells Through Regulation of KLF4 and YY1","volume":"10","author":"Vega","year":"2020","journal-title":"Front. Oncol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"5445","DOI":"10.18632\/oncotarget.23539","article-title":"MicroRNA Expression and Activity in T-Cell Acute Lymphoblastic Leukemia","volume":"9","author":"Ye","year":"2018","journal-title":"Oncotarget"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/s12943-015-0298-0","article-title":"Upregulation of the Inwardly Rectifying Potassium Channel Kir2.1 (KCNJ2) Modulates Multidrug Resistance of Small-Cell Lung Cancer under the Regulation of MiR-7 and the Ras\/MAPK Pathway","volume":"14","author":"Liu","year":"2015","journal-title":"Mol. Cancer"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"53558","DOI":"10.18632\/oncotarget.10669","article-title":"MiR-7 Reverses the Resistance to BRAFi in Melanoma by Targeting EGFR\/IGF-1R\/CRAF and Inhibiting the MAPK and PI3K\/AKT Signaling Pathways","volume":"7","author":"Sun","year":"2016","journal-title":"Oncotarget"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.cbi.2018.01.019","article-title":"MiR-7-5p Overexpression Suppresses Cell Proliferation and Promotes Apoptosis through Inhibiting the Ability of DNA Damage Repair of PARP-1 and BRCA1 in TK6 Cells Exposed to Hydroquinone","volume":"283","author":"Luo","year":"2018","journal-title":"Chem. Biol. Interact."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.celrep.2013.08.050","article-title":"An Extensive Network of TET2-Targeting MicroRNAs Regulates Malignant Hematopoiesis","volume":"5","author":"Cheng","year":"2013","journal-title":"Cell Rep."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.bbrc.2017.10.001","article-title":"MicroRNA-7 Inhibits Cell Proliferation of Chronic Myeloid Leukemia and Sensitizes It to Imatinib In Vitro","volume":"494","author":"Jiang","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Marin, A.M., Wosniaki, D.K., Sanchuki, H.B.S., Munhoz, E.C., Nardin, J.M., Soares, G.S., Espinace, D.C., Farias, J.S.d.H., Veroneze, B., and Becker, L.F. (2023). Molecular BCR::ABL1 Quantification and ABL1 Mutation Detection as Essential Tools for the Clinical Management of Chronic Myeloid Leukemia Patients: Results from a Brazilian Single-Center Study. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241210118"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","article-title":"A New Mathematical Model for Relative Quantification in Real-Time RT-PCR","volume":"29","author":"Pfaffl","year":"2001","journal-title":"Nucleic Acids Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/6\/3363\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:14:33Z","timestamp":1760105673000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/6\/3363"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,16]]},"references-count":41,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,3]]}},"alternative-id":["ijms25063363"],"URL":"https:\/\/doi.org\/10.3390\/ijms25063363","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,16]]}}}