{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T14:24:07Z","timestamp":1783607047775,"version":"3.55.0"},"reference-count":34,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,3,19]],"date-time":"2024-03-19T00:00:00Z","timestamp":1710806400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III\u2014ISCIII","doi-asserted-by":"publisher","award":["PI20\/01836"],"award-info":[{"award-number":["PI20\/01836"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III\u2014ISCIII","doi-asserted-by":"publisher","award":["CIBERESP CB06\/02\/0073"],"award-info":[{"award-number":["CIBERESP CB06\/02\/0073"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III\u2014ISCIII","doi-asserted-by":"publisher","award":["2021SGR01354"],"award-info":[{"award-number":["2021SGR01354"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"FEDER funds\/European Regional Development Fund (ERDF)","doi-asserted-by":"publisher","award":["PI20\/01836"],"award-info":[{"award-number":["PI20\/01836"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"FEDER funds\/European Regional Development Fund (ERDF)","doi-asserted-by":"publisher","award":["CIBERESP CB06\/02\/0073"],"award-info":[{"award-number":["CIBERESP CB06\/02\/0073"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"FEDER funds\/European Regional Development Fund (ERDF)","doi-asserted-by":"publisher","award":["2021SGR01354"],"award-info":[{"award-number":["2021SGR01354"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Secretariat for Universities and Research of the Ministry of Business and Knowledge of the Government of Catalonia","award":["PI20\/01836"],"award-info":[{"award-number":["PI20\/01836"]}]},{"name":"Secretariat for Universities and Research of the Ministry of Business and Knowledge of the Government of Catalonia","award":["CIBERESP CB06\/02\/0073"],"award-info":[{"award-number":["CIBERESP CB06\/02\/0073"]}]},{"name":"Secretariat for Universities and Research of the Ministry of Business and Knowledge of the Government of Catalonia","award":["2021SGR01354"],"award-info":[{"award-number":["2021SGR01354"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Elevated rates of human papillomavirus (HPV)-related anal high-grade squamous intraepithelial lesions (HSIL) and anal cancer (AC) in populations like men who have sex with men (MSM) living with HIV underscore the need for effective screening. While high-resolution anoscopy-guided biopsy is the gold standard, limited provider availability poses a challenge. This has spurred interest in identifying biomarkers for improved AC prevention. Antibodies against HPV16 oncoprotein E6, known as markers for cervical and oropharyngeal cancers, are the focus of the current study. The systematic review and meta-analysis included six studies meeting inclusion criteria, assessing HPV16 E6 seroprevalence in individuals with anal HSIL or AC. A two-step meta-analysis estimated pooled odds ratios and 95% confidence intervals (CI) for HPV16 E6 seroprevalence and HSIL or AC. Pooled prevalence, sensitivity, specificity, and diagnostic odds ratios were also calculated. This meta-analysis revealed a 3.6-fold increased risk of HSIL for HPV16 E6 seropositive individuals, escalating to a 26.1-fold risk increase for AC. Pooled specificity and sensitivity indicated a high specificity (0.99; 95%CI: 0.99, 0.99) but lower sensitivity (0.19; 95%CI: 0.10, 0.34) for HPV16 E6 serostatus as an AC biomarker. In conclusion, while HPV16 E6 seroprevalence demonstrates specificity as a potential biomarker for HPV-related AC, its utility as a standalone screening tool may be limited. Instead, it could serve effectively as a confirmation test, particularly in high-risk populations, alongside other diagnostic methods. Further research is imperative to explore HPV16 E6 seroconversion dynamics and alternative screening algorithms.<\/jats:p>","DOI":"10.3390\/ijms25063437","type":"journal-article","created":{"date-parts":[[2024,3,19]],"date-time":"2024-03-19T04:36:31Z","timestamp":1710822991000},"page":"3437","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Assessing the Potential of HPV16 E6 Seroprevalence as a Biomarker for Anal Dysplasia and Cancer Screening\u2014A Systematic Review and Meta-Analysis"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5423-7092","authenticated-orcid":false,"given":"Sara","family":"Tous","sequence":"first","affiliation":[{"name":"Cancer Epidemiology Research Programme, Catalan Institute of Oncology-IDIBELL, Av Granvia de l\u2019Hospitalet 199-203, 08908 L\u2019Hospitalet de Llobregat, Spain"},{"name":"Consortium for Biomedical Research in Epidemiology and Public Health\u2014CIBERESP, Carlos III Institute of Health, Av. De Monforte de Lemos 5, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariona","family":"Guillamet","sequence":"additional","affiliation":[{"name":"Cancer Epidemiology Research Programme, Catalan Institute of Oncology-IDIBELL, Av Granvia de l\u2019Hospitalet 199-203, 08908 L\u2019Hospitalet de Llobregat, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim","family":"Waterboer","sequence":"additional","affiliation":[{"name":"Division of Infections and Cancer Epidemiology, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laia","family":"Alemany","sequence":"additional","affiliation":[{"name":"Cancer Epidemiology Research Programme, Catalan Institute of Oncology-IDIBELL, Av Granvia de l\u2019Hospitalet 199-203, 08908 L\u2019Hospitalet de Llobregat, Spain"},{"name":"Consortium for Biomedical Research in Epidemiology and Public Health\u2014CIBERESP, Carlos III Institute of Health, Av. De Monforte de Lemos 5, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5392-953X","authenticated-orcid":false,"given":"Sonia","family":"Paytubi","sequence":"additional","affiliation":[{"name":"Cancer Epidemiology Research Programme, Catalan Institute of Oncology-IDIBELL, Av Granvia de l\u2019Hospitalet 199-203, 08908 L\u2019Hospitalet de Llobregat, Spain"},{"name":"Consortium for Biomedical Research in Epidemiology and Public Health\u2014CIBERESP, Carlos III Institute of Health, Av. 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