{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T11:19:35Z","timestamp":1792840775656,"version":"4.5.0"},"reference-count":33,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,3,20]],"date-time":"2024-03-20T00:00:00Z","timestamp":1710892800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shanghai 2022 Annual Science and Technology Innovation Action Plan Agricultural Science and Technology Field Project","award":["22N 31900500"],"award-info":[{"award-number":["22N 31900500"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Listeria monocytogenes (L. monocytogenes) is a food-borne pathogenic bacteria that frequently contaminates animal-derived food and low-temperature preserved food. Listeriosis caused by its infection has a high mortality rate and poses a serious threat to human health. Therefore, it is crucial to establish a sensitive, rapid and easy-to-operate technique. In this study, a Recombinase Aided Amplification (RAA) assisted CRISPR\/Cas12a (RAA-CRISPR\/Cas12a) fluorescence platform was established for highly sensitive nucleic acid detection of L. monocytogenes. The established RAA-CRISPR\/Cas12a showed high sensitivity and high specificity, with the sensitivity of 350 CFU\/mL and 5.4 \u00d7 10\u22123 ng\/\u03bcL for pure bacterial solution and genomic DNA, and good specificity for 5 strains of Listeria spp. and 14 strains of other common pathogenic bacteria. L. monocytogenes could be detected at an initial concentration of 2.3 CFU\/25g within 2 h of enriching the beef in the food matrix, and this method could be applied to food samples that were easily contaminated with L. monocytogenes The results of RAA-CRISPR\/Cas12a could be observed in 5 min, while the amplification was completed in 20\u201330 min. The speed and sensitivity of RAA-CRISPR\/Cas12a were significantly higher than that of the national standard method. In conclusion, the RAA-CRISPR\/Cas12a system established in this study has new application potential in the diagnosis of food-borne pathogens.<\/jats:p>","DOI":"10.3390\/ijms25063477","type":"journal-article","created":{"date-parts":[[2024,3,20]],"date-time":"2024-03-20T05:04:12Z","timestamp":1710911052000},"page":"3477","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Rapid Nucleic Acid Detection of Listeria monocytogenes Based on RAA-CRISPR Cas12a System"],"prefix":"10.3390","volume":"25","author":[{"given":"Yujuan","family":"Yang","sequence":"first","affiliation":[{"name":"School of Life Sciences, Shanghai University, Shanghai 200444, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangxiang","family":"Kong","sequence":"additional","affiliation":[{"name":"Institute of Translational Medicine, Shanghai University, Shanghai 200444, China"},{"name":"School of Medicine, Shanghai University, Shanghai 200444, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jielin","family":"Yang","sequence":"additional","affiliation":[{"name":"Technical Centre for Animal, Plant and Food Inspection and Quarantine of Shanghai Customs, Shanghai 200135, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junxin","family":"Xue","sequence":"additional","affiliation":[{"name":"Technical Centre for Animal, Plant and Food Inspection and Quarantine of Shanghai Customs, Shanghai 200135, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Niu","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Shanghai University, Shanghai 200444, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3089-9195","authenticated-orcid":false,"given":"Qin","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Shanghai University, Shanghai 200444, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Havelaar, A.H., Kirk, M.D., Torgerson, P.R., Gibb, H.J., Hald, T., Lake, R.J., Praet, N., Bellinger, D.C., De Silva, N.R., and Gargouri, N. 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