{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T22:42:38Z","timestamp":1765233758500,"version":"build-2065373602"},"reference-count":43,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T00:00:00Z","timestamp":1710979200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Next-generation sequencing is a vital tool for personalized diagnostics and therapies in cancer. Despite numerous advantages, the method depends on multiple parameters regarding the sample material, e.g., sample fixation. A panel\u2019s ability to ensure balanced pre-amplification of the regions of interest is challenging, especially in targeted sequencing approaches, but of significant importance to its applicability across hematological malignancies and solid tumors. This study comparatively evaluated the technical performance of the commercially available OncomineTM Myeloid Panel in fresh and Formalin-fixed paraffin-embedded (FFPE) material by using an Ion Torrent\u2122 Personal Genome Machine\u2122 System and Ion GeneStudio S5 System platform. In total, 114 samples were analyzed, including 55 fresh materials and 59 FFPE samples. Samples were sequenced with a minimum of one million reads. Amplicons with coverage below 400 reads were classified as underperforming. In fresh material, 49\/526 amplicons were identified as performing insufficiently, corresponding with 18 genes. Using FFPE material, 103\/526 amplicons underperformed. Independent of input material, regions in 27 genes, including ASXL1, BCOR and BRAF, did not match quality parameters. Subsequently, exemplary mutations were extracted from the Catalogue of Somatic Mutations in Cancer database. This technical evaluation of the OncomineTM Myeloid Panel identified amplicons that do not achieve adequate coverage levels and which need to be considered when interpreting sequencing.<\/jats:p>","DOI":"10.3390\/ijms25063534","type":"journal-article","created":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T04:34:46Z","timestamp":1710995686000},"page":"3534","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparative Analyses of Targeted Myeloid Cancer Next-Generation Sequencing Panel in Fresh Blood, Bone Marrow and FFPE Material"],"prefix":"10.3390","volume":"25","author":[{"given":"Andrea Daniela","family":"Hobeck","sequence":"first","affiliation":[{"name":"Clinic for Hematology, Oncology and Palliative Care, Medical Clinic III, Department of Internal Medicine, Rostock University Medical Center, Ernst-Heydemann-Strasse 6, 18057 Rostock, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophia","family":"Wendt","sequence":"additional","affiliation":[{"name":"Clinic for Hematology, Oncology and Palliative Care, Medical Clinic III, Department of Internal Medicine, Rostock University Medical Center, Ernst-Heydemann-Strasse 6, 18057 Rostock, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saskia","family":"Krohn","sequence":"additional","affiliation":[{"name":"Clinic for Hematology, Oncology and Palliative Care, Medical Clinic III, Department of Internal Medicine, Rostock University Medical Center, Ernst-Heydemann-Strasse 6, 18057 Rostock, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gudrun","family":"Knuebel","sequence":"additional","affiliation":[{"name":"Clinic for Hematology, Oncology and Palliative Care, Medical Clinic III, Department of Internal Medicine, Rostock University Medical Center, Ernst-Heydemann-Strasse 6, 18057 Rostock, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephan","family":"Bartels","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Department of Molecular Pathology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisa","family":"Schipper","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Department of Molecular Pathology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Junghanss","sequence":"additional","affiliation":[{"name":"Clinic for Hematology, Oncology and Palliative Care, Medical Clinic III, Department of Internal Medicine, Rostock University Medical Center, Ernst-Heydemann-Strasse 6, 18057 Rostock, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7123-7986","authenticated-orcid":false,"given":"Hugo","family":"Murua Escobar","sequence":"additional","affiliation":[{"name":"Clinic for Hematology, Oncology and Palliative Care, Medical Clinic III, Department of Internal Medicine, Rostock University Medical Center, Ernst-Heydemann-Strasse 6, 18057 Rostock, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4021","DOI":"10.1182\/blood-2013-09-381665","article-title":"The genetic basis of myelodysplasia and its clinical relevance","volume":"122","author":"Cazzola","year":"2013","journal-title":"Blood"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3616","DOI":"10.1182\/blood-2013-08-518886","article-title":"Clinical and biological implications of driver mutations in 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