{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T21:10:14Z","timestamp":1786741814726,"version":"build-2736575974"},"reference-count":65,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,3,24]],"date-time":"2024-03-24T00:00:00Z","timestamp":1711238400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hungarian Government"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Magnetic activated cell sorting (MACS) is a well-known sperm selection technique, which is able to remove apoptotic spermatozoa from semen samples using the classic annexinV based method. Leukocytes and erythrocytes in semen samples or in testicular tissue processed for in vitro fertilization (IVF) could exert detrimental effects on sperm. In the current study, we rethought the aforementioned technique and used magnetic microbeads conjugated with anti-CD45\/CD235a antibodies to eliminate contaminating leukocytes and erythrocytes from leukocytospermic semen samples and testicular tissue samples gained via testicular sperm extraction (TESE). With this technique, a 15.7- and a 30.8-fold reduction could be achieved in the ratio of leukocytes in semen and in the number of erythrocytes in TESE samples, respectively. Our results show that MACS is a method worth to reconsider, with more potential alternative applications. Investigations to find molecules labeling high-quality sperm population and the development of positive selection procedures based on these might be a direction of future research.<\/jats:p>","DOI":"10.3390\/ijms25073627","type":"journal-article","created":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T12:47:11Z","timestamp":1711370831000},"page":"3627","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["An Alternative Application of Magnetic-Activated Cell Sorting: CD45 and CD235a Based Purification of Semen and Testicular Tissue Samples"],"prefix":"10.3390","volume":"25","author":[{"given":"P\u00e9ter","family":"Cz\u00e9t\u00e1ny","sequence":"first","affiliation":[{"name":"Urology Clinic, University of P\u00e9cs Clinical Centre, 7621 P\u00e9cs, Hungary"},{"name":"National Laboratory on Human Reproduction, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e1s","family":"Ball\u00f3","sequence":"additional","affiliation":[{"name":"Urology Clinic, University of P\u00e9cs Clinical Centre, 7621 P\u00e9cs, Hungary"},{"name":"National Laboratory on Human Reproduction, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"},{"name":"Pannon Reproduction Institute, 8300 Tapolca, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e1szl\u00f3","family":"M\u00e1rk","sequence":"additional","affiliation":[{"name":"National Laboratory on Human Reproduction, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"},{"name":"Department of Analytical Biochemistry, Institute of Biochemistry and Medical Chemistry, University of P\u00e9cs Medical School, 7624 P\u00e9cs, Hungary"},{"name":"MTA-PTE Human Reproduction Scientific Research Group, 7624 P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9769-9396","authenticated-orcid":false,"given":"Attila","family":"T\u00f6r\u00f6k","sequence":"additional","affiliation":[{"name":"Pannon Reproduction Institute, 8300 Tapolca, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1rp\u00e1d","family":"Sz\u00e1nt\u00f3","sequence":"additional","affiliation":[{"name":"Urology Clinic, University of P\u00e9cs Clinical Centre, 7621 P\u00e9cs, Hungary"},{"name":"National Laboratory on Human Reproduction, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e1bor","family":"M\u00e1t\u00e9","sequence":"additional","affiliation":[{"name":"Urology Clinic, University of P\u00e9cs Clinical Centre, 7621 P\u00e9cs, Hungary"},{"name":"National Laboratory on Human Reproduction, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"},{"name":"Pannon Reproduction Institute, 8300 Tapolca, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"hoac051","DOI":"10.1093\/hropen\/hoac051","article-title":"Infertility Prevalence and the Methods of Estimation from 1990 to 2021: A Systematic Review and Meta-Analysis","volume":"2022","author":"Cox","year":"2022","journal-title":"Hum. 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