{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T06:31:05Z","timestamp":1785738665044,"version":"3.56.0"},"reference-count":50,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T00:00:00Z","timestamp":1711584000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>IgA vasculitis (IgAV) is the most common childhood vasculitis. The main cause of morbidity and mortality in children with IgAV is nephritis (IgAVN), but the risk of its development, severity, and chronicity remain unclear. Erythrocyte glutathione S-transferase (e-GST) activity has been previously detected as a sensitive marker of kidney function impairment in several diseases. We spectrophotometrically assessed and correlated e-GST activity between 55 IgAV patients without nephritis (IgAVwN), 42 IgAVN patients, and 52 healthy controls. At disease onset, e-GST activity was significantly higher in IgAVN patients (median (interquartile range)) (5.7 U\/gHb (4.4\u20137.5)) than in IgAVwN patients (3.1 U\/gHb (2.2\u20134.2); p &lt; 0.001), and controls (3.1 U\/gHb (1.9\u20134.2); p &lt; 0.001). Therewithal, there were no differences between the IgAVwN patients and controls (p = 0.837). e-GST activity was also significantly higher in the IgAVN patients than in the IgAVwN patients after 3 months (5.0 U\/gHb (4.2\u20136.2) vs. 3.3 U\/gHb (2.3\u20134.1); p &lt; 0.001) and 6 months (4.2 U\/gHb (3.2\u20135.8) vs. 3.3 U\/gHb (2.1\u20134.1); p &lt; 0.001) since the disease onset. Consistent correlations between e-GST activity and serum creatinine, estimated glomerular filtration rate (eGFR), and proteinuria levels were not detected. In conclusion, increased e-GST activity can serve as a subtle indicator of kidney function impairment in children with IgAV.<\/jats:p>","DOI":"10.3390\/ijms25073795","type":"journal-article","created":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T12:52:12Z","timestamp":1711630332000},"page":"3795","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Erythrocyte Glutathione S-Transferase Activity as a Sensitive Marker of Kidney Function Impairment in Children with IgA Vasculitis"],"prefix":"10.3390","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1520-3375","authenticated-orcid":false,"given":"Marijan","family":"Frkovic","sequence":"first","affiliation":[{"name":"Department of Pediatrics, University Hospital Centre Zagreb, University of Zagreb School of Medicine, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8138-4170","authenticated-orcid":false,"given":"Ana","family":"Turcic","sequence":"additional","affiliation":[{"name":"Department of Laboratory Diagnostics, University Hospital Centre Zagreb, University of Zagreb Faculty of Pharmacy and Biochemistry, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alenka","family":"Gagro","sequence":"additional","affiliation":[{"name":"Children\u2019s Hospital Zagreb, Medical Faculty Osijek, Josip Juraj Strossmayer University of Osijek, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6555-2613","authenticated-orcid":false,"given":"Sasa","family":"Srsen","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University Hospital Centre Split, School of Medicine, University of Split, 21000 Split, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1513-2965","authenticated-orcid":false,"given":"Sanda Huljev","family":"Frkovic","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University Hospital Centre Zagreb, University of Zagreb School of Medicine, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dunja","family":"Rogic","sequence":"additional","affiliation":[{"name":"Department of Laboratory Diagnostics, University Hospital Centre Zagreb, University of Zagreb Faculty of Pharmacy and Biochemistry, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1728-4260","authenticated-orcid":false,"given":"Marija","family":"Jelusic","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University Hospital Centre Zagreb, University of Zagreb School of Medicine, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1016\/j.autrev.2017.10.009","article-title":"New insights in the pathogenesis of immunoglobulin A vasculitis (Henoch-Sch\u00f6nlein purpura)","volume":"16","author":"Heineke","year":"2017","journal-title":"Autoimmun. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.1093\/rheumatology\/kez041","article-title":"European consensus-based recommendations for diagnosis and treatment of immunoglobulin A vasculitis\u2014The SHARE initiative","volume":"58","author":"Ozen","year":"2019","journal-title":"Rheumatology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"162","DOI":"10.5114\/ceji.2018.77386","article-title":"Serum GDIgA1 levels in children with IgA nephropathy and Henoch-Sch\u00f6nlein nephritis","volume":"43","author":"Gajewski","year":"2018","journal-title":"Cent. Eur. J. Immunol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1136\/annrheumdis-2020-218649","article-title":"Geospatial clustering of childhood IgA vasculitis and IgA vasculitis-associated nephritis","volume":"80","author":"Sapina","year":"2021","journal-title":"Ann. Rheum. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1136\/ard.2009.116657","article-title":"Paediatric Rheumatology International Trials Organisation (PRINTO)\/EULAR\/PRINTO\/PRES criteria for Henoch-Schonlein purpura, childhood polyarteritis nodosa, childhood Wegener granulomatosis and childhood Takayasu arteritis: Ankara 2008. part II: Final classification criteria","volume":"69","author":"Ozen","year":"2010","journal-title":"Ann. Rheum. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1097\/BOR.0b013e32833af608","article-title":"Henoch-Sch\u00f6nlein purpura","volume":"22","author":"Saulsbury","year":"2010","journal-title":"Curr. Opin. Rheumatol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"42","DOI":"10.5415\/apallergy.2014.4.1.42","article-title":"Age of onset as a risk factor of renal involvement in Henoch-Sch\u00f6nlein purpura","volume":"4","author":"Ghrahani","year":"2014","journal-title":"Asia Pac. Allergy"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1007\/s10157-015-1177-0","article-title":"Clinical manifestations of Henoch-Sch\u00f6nlein purpura nephritis and IgA nephropathy: Comparative analysis of data from the Japan Renal Biopsy Registry (J-RBR)","volume":"20","author":"Komatsu","year":"2016","journal-title":"Clin. Exp. Nephrol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s12969-019-0311-z","article-title":"Different histological classifications for Henoch-Sch\u00f6nlein purpura nephritis: Which one should be used?","volume":"17","author":"Jelusic","year":"2019","journal-title":"Pediatr. Rheumatol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Pillebout, E., Jamin, A., Ayari, H., Housset, P., Pierre, M., Sauvaget, V., Viglietti, D., Deschenes, G., Monteiro, R.C., and Berthelot, L. (2017). Biomarkers of IgA vasculitis nephritis in children. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0188718"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1038\/nrneph.2014.126","article-title":"Henoch-Sch\u00f6nlein purpura nephritis in children","volume":"10","author":"Davin","year":"2014","journal-title":"Nat. Rev. Nephrol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1007\/s00467-017-3604-9","article-title":"Clinical outcomes in children with Henoch-Sch\u00f6nlein purpura nephritis without crescents","volume":"32","author":"Delbet","year":"2017","journal-title":"Pediatr. Nephrol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1007\/s00467-017-3608-5","article-title":"The ISKDC classification and a new semiquantitative classification for predicting outcomes of HenochSch\u00f6nlein purpura nephritis","volume":"32","author":"Koskela","year":"2017","journal-title":"Pediatr. Nephrol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s00467-014-2815-6","article-title":"Henoch-Sch\u00f6nlein purpura nephritis","volume":"30","author":"Pohl","year":"2015","journal-title":"Pediatr. Nephrol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1007\/s10157-011-0478-1","article-title":"The pathogenesis and treatment of pediatric Henoch-Sch\u00f6nlein purpura nephritis","volume":"15","author":"Kawasaki","year":"2011","journal-title":"Clin. Exp. Nephrol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"15","DOI":"10.5387\/fms.59.15","article-title":"Henoch-Sch\u00f6nlein purpura nephritis in childhood: Pathogenesis, prognostic factors and treatment","volume":"59","author":"Kawasaki","year":"2013","journal-title":"Fukushima J. Med. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1007\/s11926-018-0735-3","article-title":"IgA Vasculitis: Genetics and Clinical and Therapeutic Management","volume":"20","author":"Pina","year":"2018","journal-title":"Curr. Rheumatol. Rep."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Wada, Y., Matsumoto, K., Suzuki, T., Saito, T., Kanazawa, N., Tachibana, S., Iseri, K., Sugiyama, M., Iyoda, M., and Shibata, T. (2018). Clinical significance of serum and mesangial galactose-deficient IgA1 in patients with IgA nephropathy. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0206865"},{"key":"ref_19","first-page":"258","article-title":"Urinary Macrophage Migration Inhibitory Factor as a Noninvasive Biomarker in Pediatric Henoch-Sch\u00f6nlein Purpura Nephritis","volume":"23","author":"Wang","year":"2017","journal-title":"Am. J. Clin. Oncol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.3892\/etm.2014.1595","article-title":"Renal neutrophil gelatinaseassociated lipocalin and kidney injury molecule-1 expression in children with acute kidney injury and Henoch-Sch\u00f6nlein purpura nephritis","volume":"7","author":"Du","year":"2014","journal-title":"Exp. Ther. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1007\/s00431-013-2150-0","article-title":"Pentraxin 3 as a novel early biomarker for the prediction of Henoch-Sch\u00f6nlein purpura nephritis in children","volume":"173","author":"Ge","year":"2014","journal-title":"Eur. J. Pediatr."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/j.clinbiochem.2013.01.017","article-title":"Soluble transferrin receptor in urine, a new biomarker for IgA nephropathy and Henoch-Sch\u00f6nlein purpura nephritis","volume":"46","author":"Delanghe","year":"2013","journal-title":"Clin. Biochem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"425","DOI":"10.3109\/0886022X.2012.757826","article-title":"Association of matrix metalloproteinase-9 level with the risk of renal involvement for Henoch-Sch\u00f6nlein purpura in children","volume":"35","author":"Zhou","year":"2013","journal-title":"Ren. Fail."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1111\/j.1440-1797.2011.01515.x","article-title":"Urinary angiotensinogen levels in relation to renal involvement of Henoch-Schonlein purpura in children","volume":"17","author":"Mao","year":"2012","journal-title":"Nephrology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.trsl.2012.09.001","article-title":"Serum levels of alpha-smooth muscle actin and c-Met as biomarkers of the degree of severity of Henoch-Schonlein purpura nephritis","volume":"161","author":"Zhang","year":"2013","journal-title":"Transl. Res."},{"key":"ref_26","first-page":"161","article-title":"Podocalyxin expression in renal tissues and correlation with the number of urinary podocytes in children with Henoch-Schonlein purpura nephritis","volume":"37","author":"Huang","year":"2012","journal-title":"J. Cent. South Univ. Med. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1007\/s00467-013-2740-0","article-title":"Urinary MCP-1\/creatinine in Henoch-Sch\u00f6nlein purpura and its relationship with nephritis","volume":"29","author":"Fuentes","year":"2014","journal-title":"Pediatr. Nephrol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"103","DOI":"10.5507\/bp.2010.017","article-title":"Phase II drug metabolizing enzymes","volume":"154","author":"Jancova","year":"2010","journal-title":"Biomed. Pap."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/s00592-013-0497-3","article-title":"Erythrocyte glutathione transferase activity: A possible early biomarker for blood toxicity in uremic diabetic patients","volume":"51","author":"Noce","year":"2014","journal-title":"Acta Diabetol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1177\/0394632015572564","article-title":"The possible role of glutathione-S-transferase activity in diabetic nephropathy","volume":"28","author":"Tesauro","year":"2015","journal-title":"Int. J. Immunopathol. Pharmacol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e377","DOI":"10.1038\/cddis.2012.112","article-title":"Erythrocyte glutathione transferase: A new biomarker for hemodialysis adequacy, overcoming the Kt\/V(urea) dogma?","volume":"3","author":"Noce","year":"2012","journal-title":"Cell Death Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1038\/s41419-018-0289-3","article-title":"Erythrocyte glutathione transferase in kidney transplantation: A probe for kidney detoxification efficiency","volume":"9","author":"Bocedi","year":"2018","journal-title":"Cell Death Dis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1007\/s00726-011-1085-x","article-title":"Erythrocyte glutathione transferase: A potential new biomarker in chronic kidney diseases which correlates with plasma homocysteine","volume":"43","author":"Noce","year":"2012","journal-title":"Amino Acids"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e736","DOI":"10.1038\/cddis.2013.255","article-title":"Erythrocyte glutathione transferase: A non-antibody biomarker for systemic sclerosis, which correlates with severity and activity of the disease","volume":"4","author":"Fabrini","year":"2013","journal-title":"Cell Death Dis."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/s12969-017-0146-4","article-title":"A single-center analysis of HenochSchonlein purpura nephritis with nephrotic proteinuria in children","volume":"15","author":"Feng","year":"2017","journal-title":"Pediatr. Rheumatol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"679","DOI":"10.2215\/CJN.06710810","article-title":"Henoch-Schonlein purpura nephritis: Pathophysiology, treatment, and future strategy","volume":"6","author":"Davin","year":"2011","journal-title":"Clin. J. Am. Soc. Nephrol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1186\/s13052-018-0538-1","article-title":"Significance of glomerular fibrinogen deposition in children with Henoch-Sch\u00f6nlein purpura nephritis","volume":"44","author":"Wang","year":"2018","journal-title":"Ital. J. Pediatr."},{"key":"ref_38","first-page":"1579","article-title":"Value of biomarkers for predicting immunoglobulin A vasculitis nephritis outcome in an adult prospective cohort","volume":"33","author":"Berthelot","year":"2018","journal-title":"Nephrol. Dial. Transplant."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1007\/s00431-015-2601-x","article-title":"Beneficial effects of creatine phosphate sodium for the treatment of Henoch-Sch\u00f6nlein purpura in patients with early renal damage detected using urinary kidney injury molecule-1 levels","volume":"175","author":"Zhang","year":"2016","journal-title":"Eur. J. Pediatr."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/s00467-009-1230-x","article-title":"Pathogenesis of Henoch-Sch\u00f6nlein purpura nephritis","volume":"25","author":"Lau","year":"2010","journal-title":"Pediatr. Nephrol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1100\/tsw.2007.23","article-title":"Therapy for children with henochschonlein purpura nephritis: A systematic review","volume":"7","author":"Zaffanello","year":"2007","journal-title":"Sci. World J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1111\/jpc.12403","article-title":"Henoch-Sch\u00f6nlein purpura in children","volume":"49","author":"Trnka","year":"2013","journal-title":"J. Paediatr. Child Health"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1159\/000365692","article-title":"Urological Manifestations of Henoch-Schonlein Purpura: A Review","volume":"8","author":"Dalpiaz","year":"2015","journal-title":"Curr. Urol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1586\/1744666X.2013.850028","article-title":"Advances in our understanding of the patogenesis of Henoch-Sch\u00f6nlein purpura and the implications for improving its diagnosis","volume":"9","author":"Park","year":"2013","journal-title":"Expert Rev. Clin. Immunol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Chan, H., Tang, Y.L., Lv, X.H., Zhang, G.F., Wang, M., Yang, H.P., and Li, Q. (2016). Risk Factors Associated with Renal Involvement in Childhood Henoch-Sch\u00f6nlein Purpura: A Meta-Analysis. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0167346"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"117","DOI":"10.3389\/fimmu.2016.00117","article-title":"The Origin and Activities of IgA1-Containing Immune Complexes in IgA Nephropathy","volume":"7","author":"Knoppova","year":"2016","journal-title":"Front. Immunol."},{"key":"ref_47","first-page":"88","article-title":"Preliminary study of Erythrocyte glutathione-s-transferase activity in patients with skin melanoma","volume":"10","author":"Anastasov","year":"2012","journal-title":"Trakia J. Sci."},{"key":"ref_48","first-page":"127","article-title":"Erythrocyte Glutathione-S-Transferase Activity in Diabetics and Its Association with HBA1c","volume":"24","author":"Orhan","year":"1999","journal-title":"J. Pharm. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.bbrc.2012.08.037","article-title":"Erythrocyte glutathione transferase: A novel biomarker to check environmental pollution hazardous for humans","volume":"426","author":"Fabrini","year":"2012","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1093\/ndt\/gfs369","article-title":"Glutathione S-transferase A1, M1, P1 and T1 null or low-activity genotypes are associated with enhanced oxidative damage among haemodialysis patients","volume":"28","author":"Suvakov","year":"2013","journal-title":"Nephrol. Dial. Transplant."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/7\/3795\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:20:20Z","timestamp":1760106020000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/25\/7\/3795"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,28]]},"references-count":50,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2024,4]]}},"alternative-id":["ijms25073795"],"URL":"https:\/\/doi.org\/10.3390\/ijms25073795","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,28]]}}}